MicroRNA-214 suppresses osteogenic differentiation of C2C12 myoblast cells by targeting Osterix

Shi, Kaikai; Lu, Jiajia; Zhao, Yue; Wang, Lintao; Li, Ji; Qi, Bing; Li, Hongwei; Ma, Chao

doi:10.1016/j.bone.2013.04.002

Cited by 118 publications

(78 citation statements)

References 49 publications

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“…During osteoblast mineralization, Osx can bind to the miR-93 promoter to repress its transcription and, as miR-93 also targets Osx mRNA, this facilitates the maintenance of osterix levels (Yang et al 2012). Shi et al (2013) have also reported the downregulation of miR-93 expression during the differentiation of C2C12 cells under BMP stimulation. Fine-tuning of Osx expression by miRNAs is also observed in the miR-322/Tob2 feedback mechanism in vitro.…”

Section: Mirnas and Osteoblast Differentiationmentioning

confidence: 97%

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MicroRNAs and post-transcriptional regulation of skeletal development

Gámez¹,

Rodríguez-Carballo²,

Ventura³

2014

Journal of Molecular Endocrinology

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MicroRNAs (miRNAs) have become integral nodes of post-transcriptional control of genes that confer cellular identity and regulate differentiation. Cell-specific signaling and transcriptional regulation in skeletal biology are extremely dynamic processes that are highly reliant on dose-dependent responses. As such, skeletal cell-determining genes are ideal targets for quantitative regulation by miRNAs. So far, large amounts of evidence have revealed a characteristic temporal miRNA signature in skeletal cell differentiation and confirmed the essential roles that numerous miRNAs play in bone development and homeostasis. In addition, microarray expression data have provided evidence for their role in several skeletal pathologies. Mouse models in which their expression is altered have provided evidence of causal links between miRNAs and bone abnormalities. Thus, a detailed understanding of the function of miRNAs and their tight relationship with bone diseases would constitute a powerful tool for early diagnosis and future therapeutic approaches.

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Section: Mirnas and Osteoblast Differentiationmentioning

confidence: 97%

“…Shi et al (2013) described miR-214 as a downregulated miRNA during BMP2-induced osteoblast differentiation in C2C12 cells. miR-214 antagomirs lead to the overexpression of Osx and other related osteoblast markers such as Alpl, Col1a1, and Bglap.…”

Section: Mirnas and Osteoblast Differentiationmentioning

confidence: 99%

MicroRNAs and post-transcriptional regulation of skeletal development

Gámez¹,

Rodríguez-Carballo²,

Ventura³

2014

Journal of Molecular Endocrinology

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“…74 Recently, miR214 has been shown to inhibit bone formation in human cell lines both by targeting ATF4, a gene encoding one of the main transcription factors required for osteoblast function 31 and by suppressing osteogenic differentiation of C2C12 myoblast cells by targeting SP7, an osteoblast-specific transcription factor. 30,75 To predict putative mRNA targets for miR199 and miR214, we used miRZ 91 for mouse and human and TargetScanFish 92 for zebrafish. We then narrowed down the list by keeping only the genes having GO term associated with the skeleton in at least one of the three species.…”

Section: Discussionmentioning

confidence: 99%

“…[22][23][24] miR199 and miR214 play roles in the differentiation of mammalian skeletal precursor cells into osteoblasts or chondrocytes. [25][26][27][28][29][30][31] In addition, these miRs function in the development of muscle and heart, [32][33][34][35] and regulate the development and progression of various cancers. [36][37][38][39][40] The three columns containing all gaps were inserted to separate the two mature sequences visually.…”

Section: Introductionmentioning

confidence: 99%

Evolution of themiR199-214cluster and vertebrate skeletal development

2014

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The miR199 and miR214 genes occupy an intronic cluster located on the opposite strand of the Dynamin3 gene. These miRNAs play major roles in a broad variety of developmental processes and diseases, including skeletal development and several types of cancer. In the work reported here, we first deciphered the origin of the miR199 and miR214 families by following evolution of miR paralogs and their host Dynamin paralogs. We then examined the expression patterns of miR199 and miR214 in developing zebrafish embryos and demonstrated their regulation through a common primary transcript. Results suggest an evolutionarily conserved regulation across vertebrate lineages. Our expression study showed predominant expression patterns for both miR in tissues surrounding developing craniofacial skeletal elements consistent with expression data in mouse and human, thus indicating a conserved role of miR199 and miR214 in vertebrate skeletogenesis.

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“…In recent years, there have been researches focusing on the correlation of miR-214 with bone formation [17][18][19]. Among, one study demonstrated that miR-214 directly targeted ATF4 to inhibit osteoblast activity [17].…”

Section: Introductionmentioning

confidence: 99%

MiR-214 Attenuates Osteogenic Differentiation of Mesenchymal Stem Cells via Targeting FGFR1

Yang

Ge²,

Cao³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Postmenopausal osteoporosis is closely associated with reduction in the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. Previous studies have demonstrated that miR-214 plays an important role in the genesis and development of postmenopausal osteoporosis. Here, we performed this study to investigate the potential mechanism by which miR-214 regulates osteoblast differentiation of MSCs. Methods: First, we explored the expression of miR-214 in MSCs of osteoporotic mice. Next, we examined the change of miR-214 during osteoblast differentiation of MSCs. Then, MSCs were infected with lentiviral vectors expressing miR-214 or miR-214 sponge to investigate the effect of miR-214 on osteoblast differentiation of MSCs. Further, bioinformatics analysis and luciferase reporter assay were performed to identify and validate the target gene of miR-214. Results: MiR-214 was up-regulated in MSCs of osteoporotic mice and down-regulated during osteoblast differentiation of MSCs. Furthermore, overexpression of miR-214 inhibited osteoblast differentiation of MSCs in vitro, whereas inhibition of miR-214 function promoted this process, evidenced by increased expression of osteoblast-specific genes, alkaline phosphatase (ALP) activity, and matrix mineralization. Bioinformatics, Western blot analysis and luciferase reporter assay demonstrated that FGFR1 is a direct target of miR-214. Conclusions: MiR-214 attenuates osteogenesis by inhibiting the FGFR1/FGF signaling pathway. Our findings suggest that targeting miR-214 promises to be a potential therapy in treatment of postmenopausal osteoporosis.L. Yang and D. Ge contributed to the work equally.

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MicroRNA-214 suppresses osteogenic differentiation of C2C12 myoblast cells by targeting Osterix

Cited by 118 publications

References 49 publications

MicroRNAs and post-transcriptional regulation of skeletal development

MicroRNAs and post-transcriptional regulation of skeletal development

Evolution of themiR199-214cluster and vertebrate skeletal development

MiR-214 Attenuates Osteogenic Differentiation of Mesenchymal Stem Cells via Targeting FGFR1

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