MicroRNA-21 deficiency attenuated atherogenesis and decreased macrophage infiltration by targeting Dusp-8

Gao, Lin; Zeng, Huasu; Zhang, Tiantian; Mao, Chengyu; Wang, Yue; Han, Zhihua; Chen, Kan; Zhang, Junfeng; Fan, Yuqi; Gu, Jun; Wang, Changqian

doi:10.1016/j.atherosclerosis.2019.10.003

Cited by 30 publications

(12 citation statements)

References 45 publications

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“…miR-126 is an important regulator of EC biology, including angiogenesis, vascular repair, inflammation activation and apoptosis ( 27 ). MiR-21 plays an important role in the regulation of cardiovascular diseases, including changes in the functions of ECs, vascular smooth muscle cells and macrophage-foam cells ( 28 ). miR-210 can modulate the angiogenesis of ECs upon hypoxic condition via vascular endothelial growth factor (VEGF) signaling ( 29 ), miR-221/-222 can inhibit the migration, proliferation and angiogenesis of ECs ( 25 ), miR-150 can promote EC proliferation and plays an important role in angiogenesis and proliferation ( 30 ).…”

Section: Introductionmentioning

confidence: 99%

Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism

Yao

Wang

et al. 2022

Front. Endocrinol.

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BackgroundIncreasing evidence has shown that elevated Thyroid stimulating hormone (TSH) levels are positively correlated with atherosclerosis (ATH) in patients with subclinical hypothyroidism (SCH). Some researchers found that the dysfunction of Endothelial Cells (ECs) in SCH plays an important role in the pathogenesis of ATH in SCH, but the association remains controversial.ObjectivesTo determine the expression profiles of serum microRNAs critical to the function of Endothelial cells (ECs) may help reanalyze the possible mechanism underlying ATH in SCH and the association between ATH and SCH.MethodsWe used qRT-PCR to perform microRNA profiling and analysis in normal control subjects (NC), patients with SCH alone (SCH), patients with SCH and ATH (SCH+ATH), and patients with ATH without SCH (ATH).ResultsBoth miR-221-3p and miR-222-3p showed a decreasing expression trend between the SCH and SCH+ATH groups. In addition, miR-126-3p and miR-150-5p showed a stepwise decrease from the NC to SCH groups and then to the SCH+ATH or ATH group. miR-21-5p was unregulated in the SCH, SCH+ATH, and ATH groups. Furthermore, elevated levels of miR-21-5p in SCH+ATH group were higher than SCH and ATH group. No differences were found in the levels of miR-150, miR-126, miR-221 and miR-222 between the ATH and the SCH+ATH subjects.ConclusionsmiR-21-5p may be involved in the atherosclerosis process in patients with SCH (SCH and SCH+ATH groups). miR-150-5p may be sensitive risk markers for predicting endothelial dysfunction in patients with ATH (ATH and SCH+ATH groups).

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Section: Introductionmentioning

confidence: 99%

Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism

Yao

Wang

et al. 2022

Front. Endocrinol.

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“…In according to previous publications, we also confirmed that TND could play a beneficial role via increasing the antioxidant enzymes. In addition, macrophage activation was an important pathophysiological process in atherosclerosis [ 21 ]. In our study, we observed that TND alleviated macrophage infiltration, restricting the formation of atherosclerotic plaques [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Tongnao Decoction (TND) Alleviated Atherosclerosis by Playing Lowering Lipid, Anti-Inflammatory, and Antioxidant Roles

Jiang

Wang

Sheng

et al. 2022

Oxidative Medicine and Cellular Longevity

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Background. Tongnao decoction (TND) has been extensively prescribed for the treatment of stroke. However, little is known about the role of TND in the progression of carotid atherosclerosis. Methods. A mouse carotid atherosclerosis model was established with a silastic collar placed around the right common carotid artery and fed on Western diet for 12 weeks. The treatment group was given a gavage of TND at a dose of 1 mg/kg/d. The atherosclerotic lesion size and the compositions were observed using Oil red O staining and immunofluorescent staining. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the levels of lipid profiles, oxidative stress, and inflammatory factors. Human aortic endothelial cells (HAoECs) were treated with oxLDL with or without TND for in vitro experiments. Results. TND treatment significantly suppressed the progression of atherosclerosis, as characterized with a smaller lesion size, less percentage of vascular smooth muscle cell proliferation, endothelial cell apoptosis, and macrophage infiltration. In addition, TND decreased the levels of lipid profiles, oxidative stress, and inflammatory factors in atherosclerosis. In vitro results showed that TND inhibited the apoptosis of endothelial cells via activating ERK and AKT pathway. Conclusions. Our study demonstrated that TND significantly protected from atherosclerosis via promoting endothelial cell survival and alleviating oxidative stress and inflammatory response, which may have become a treatment in atherosclerotic diseases.

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“…Therapeutic approaches for blocking CCR2/CCL2-mediated monocytes recruitment include using siRNAs [ 143 ], monoclonal antibodies, CCR2 antagonists, pharmacological inhibition [ 144 ], and MCP-1 inhibitors.…”

Section: Potential Therapeutic Approaches Targeting Plaque Macrophagesmentioning

confidence: 99%

The Entry and Egress of Monocytes in Atherosclerosis: A Biochemical and Biomechanical Driven Process

Xiong

et al. 2021

Cardiovascular Therapeutics

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In accordance with “the response to injury” theory, the entry of monocytes into the intima guided by inflammation signals, taking up cholesterol and transforming into foam cells, and egress from plaques determines the progression of atherosclerosis. Multiple cytokines and receptors have been reported to be involved in monocyte recruitment such as CCL2/CCR2, CCL5/CCR5, and CX3CL1/CX3CR1, and the egress of macrophages from the plaque like CCR7/CCL19/CCL21. Interestingly, some neural guidance molecules such as Netrin-1 and Semaphorin 3E have been demonstrated to show an inhibitory effect on monocyte migration. During the processes of monocytes recruitment and migration, factors affecting the biomechanical properties (e.g., the membrane fluidity, the deformability, and stiffness) of the monocytes, like cholesterol, amyloid-β peptide (Aβ), and lipopolysaccharides (LPS), as well as the biomechanical environment that the monocytes are exposed, like the extracellular matrix stiffness, mechanical stretch, blood flow, and hypertension, were discussed in the latter section. Till now, several small interfering RNAs (siRNAs), monoclonal antibodies, and antagonists for CCR2 have been designed and shown promising efficiency on atherosclerosis therapy. Seeking more possible biochemical factors that are chemotactic or can affect the biomechanical properties of monocytes, and uncovering the underlying mechanism, will be helpful in future studies.

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MicroRNA-21 deficiency attenuated atherogenesis and decreased macrophage infiltration by targeting Dusp-8

Cited by 30 publications

References 45 publications

Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism

Expression patterns of serum MicroRNAs related to endothelial dysfunction in patients with subclinical hypothyroidism

Tongnao Decoction (TND) Alleviated Atherosclerosis by Playing Lowering Lipid, Anti-Inflammatory, and Antioxidant Roles

The Entry and Egress of Monocytes in Atherosclerosis: A Biochemical and Biomechanical Driven Process

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