MicroRNA–21 as a novel biomarker in diagnosis and response to therapy in asthmatic children

Elbehidy, Rabab Mohamed; Youssef, Doaa Mohammed; El‐Shal, Amal S.; Shalaby, Sally M.; Sherbiny, Hanan S.; Sherief, Laila M.; Akeel, Nagwa E.

doi:10.1016/j.molimm.2015.12.015

Cited by 74 publications

(70 citation statements)

References 48 publications

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“…In addition, miR-223, miR-142 and miR-629 levels in sputum were associated with asthma disease severity (Maes et al, 2016), and decreased miR-192 expression in peripheral blood mononuclear cells was observed 2 h following allergen challenge (Yamamoto et al, 2012). In concordance with data on IPF and COPD, elevated serum levels of miR-21 were also reported in asthma and were predictive of responsiveness to steroid treatment (Elbehidy et al, 2016).…”

Section: Clinical Use Of Mirnas In Respiratory Disease Mirnas As Biomsupporting

confidence: 68%

The role of microRNAs in chronic respiratory disease: recent insights

Stolzenburg¹,

Harris²

2017

Biological Chemistry

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Chronic respiratory diseases encompass a group of diverse conditions affecting the airways, which all impair lung function over time. They include cystic fibrosis (CF), idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD) and asthma, which together affect hundreds of millions of people worldwide. MicroRNAs (miRNAs), a class of small non-coding RNAs involved in post-transcriptional gene repression, are now recognized as major regulators in the development and progression of chronic lung disease. Alterations in miRNA abundance occur in lung tissue, inflammatory cells, and freely circulating in blood and are thought to function both as drivers and modifiers of disease. Their importance in lung pathology has prompted the development of miRNA-based therapies and biomarker tools. Here, we review the current literature on miRNA expression and function in chronic respiratory disease and highlight further research that is needed to propel miRNA treatments for lung disorders towards the clinic.

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Section: Clinical Use Of Mirnas In Respiratory Disease Mirnas As Biomsupporting

confidence: 68%

The role of microRNAs in chronic respiratory disease: recent insights

Stolzenburg¹,

Harris²

2017

Biological Chemistry

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“…Elbehidy et al (106) showed that miR-21 expression is higher in asthmatic patients than in controls and was also higher in steroid-resistant asthmatics compared with steroid-sensitive asthmatics. Moreover, IL-12p35 serum levels were inversely correlated with miR-21 levels, showing a possible role in asthma development through IL-12p35 repression.…”

Section: Mirnas and Asthmamentioning

confidence: 99%

“…Moreover, IL-12p35 serum levels were inversely correlated with miR-21 levels, showing a possible role in asthma development through IL-12p35 repression. Likewise, the ROC curve analysis showed that miR-21 expression levels could be used to differentiate asthmatic from healthy children and to monitor the response to corticosteroid treatment (106). Another group has shown that miR-21 expression was elevated in asthmatics’ serum compared to controls, although they demonstrated that miR-21 expression in serum does not correlate with serum IgE levels (107).…”

Section: Mirnas and Asthmamentioning

confidence: 99%

Novel Modulators of Asthma and Allergy: Exosomes and MicroRNAs

et al. 2017

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Intercellular communication is crucial to the immune system response. In the recent years, the discovery of exosomes has changed the way immune response orchestration was understood. Exosomes are able to operate as independent units that act as mediators in both physiological and pathological conditions. These structures contain proteins, lipidic mediators, and nucleic acids and notoriously include microRNAs (miRNAs). miRNAs are short RNA sequences (around 19–22 nucleotides) with a high phylogenetic conservation and can partially or totally regulate multiple mRNAs, inhibiting protein synthesis. In respiratory diseases such as asthma and allergic sensitization, exosomes released by several cell types and their specific content perform crucial functions in the development and continuation of the pathogenic mechanisms. Released exosomes and miRNAs inside them have been found in different types of clinical samples, such as bronchoalveolar lavage fluids and sputum supernatants, providing new data about the environmental factors and mediators that participate in the inflammatory responses that lead to the exacerbation of asthma. In this review, we summarize our current knowledge of the role of exosomes and miRNAs in asthma and allergic sensitization, paying attention to the functions that both exosomes and miRNAs are described to perform through the literature. We review the effect of exosomes and miRNAs in cells implicated in asthma pathology and the genes and pathways that they modify in them, depicting how their behavior is altered in disease status. We also describe their possible repercussion in asthma diagnosis through their possible role as biomarkers. Therefore, both exosomes and miRNAs can be viewed as potential tools to be added to the arsenal of therapeutics to treat this disease.

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“…For example, a recent study of miRNA expression profiles in IL-22and IL-17-positive T cells sorted from human PBMCs showed higher expression levels of miR-323-3p in asthmatics and a reverse correlation between miR-323-3p levels and IL-22 production, suggesting a negative feedback mechanism of miR-323-3p that controls the production of IL-22 in IL-22/IL-17-producing T cells in asthma [46]. Serum miR-21 was identified to be associated with childhood asthma or allergic inflammation and may server as a biomarker for asthma in children [47][48][49]. For example, a study performed in 95 asthmatic children and 80 healthy children identified serum miR-21 level was higher in asthmatics compared with controls, and higher level was also found in asthmatics without ICS or in steroid-resistant patients compared to steroid-sensitive children, which indicate miR-21 may also help to distinguish ICS-sensitive and ICS-resistant asthma patients [48].…”

Section: Studies Of Noncoding Rna and Asthma Developmentmentioning

confidence: 99%

“…Serum miR-21 was identified to be associated with childhood asthma or allergic inflammation and may server as a biomarker for asthma in children [47][48][49]. For example, a study performed in 95 asthmatic children and 80 healthy children identified serum miR-21 level was higher in asthmatics compared with controls, and higher level was also found in asthmatics without ICS or in steroid-resistant patients compared to steroid-sensitive children, which indicate miR-21 may also help to distinguish ICS-sensitive and ICS-resistant asthma patients [48]. Similarly, a recent miRNA study preformed in 27 asthmatic children and 21 healthy controls also revealed the increased level of plasma miR-21 and miR-146a in asthmatics compared with controls.…”

Section: Studies Of Noncoding Rna and Asthma Developmentmentioning

confidence: 99%

The role of epigenetics in the development of childhood asthma

Koppelman

2019

Expert Review of Clinical Immunology

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Introduction: The development of childhood asthma is caused by a combination of genetic factors and environmental exposures. Epigenetics describes mechanisms of (heritable) regulation of gene expression that occur without changes in DNA sequence. Epigenetics is strongly related to aging, is cell-type specific, and includes DNA methylation, noncoding RNAs, and histone modifications. Areas covered: This review summarizes recent epigenetic studies of childhood asthma in humans, which mostly involve studies of DNA methylation published in the recent five years. Environmental exposures, in particular cigarette smoking, have significant impact on epigenetic changes, but few of these epigenetic signals are also associated with asthma. Several asthma-associated genetic variants relate to DNA methylation. Epigenetic signals can be better understood by studying their correlation with gene expression, which revealed higher presence and activation of blood eosinophils in asthma. Strong associations of nasal methylation signatures and atopic asthma were identified, which were replicable across different populations. Expert commentary: Epigenetic markers have been strongly associated with asthma, and might serve as biomarker of asthma. The causal and longitudinal relationships between epigenetics and disease, and between environmental exposures and epigenetic changes need to be further investigated. Efforts should be made to understand cell-type-specific epigenetic mechanisms in asthma.

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MicroRNA–21 as a novel biomarker in diagnosis and response to therapy in asthmatic children

Cited by 74 publications

References 48 publications

The role of microRNAs in chronic respiratory disease: recent insights

The role of microRNAs in chronic respiratory disease: recent insights

Novel Modulators of Asthma and Allergy: Exosomes and MicroRNAs

The role of epigenetics in the development of childhood asthma

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