MicroRNA-205 functions as a tumor suppressor in human glioblastoma cells by targeting VEGF-A

Xiao, Yue; Wang, Peiguo; Xu, Jun; Zhu, Yufang; Sun, Guan; Pang, Qi; Tao, Rui

doi:10.3892/or.2011.1588

Cited by 91 publications

(91 citation statements)

References 35 publications

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“…In addition to miR-29b, the VEGF transcript is predicted to contain binding sites for multiple miRNA types (as highlighted in Fig. 2C), such as miR-145 and miR-205, the expressions of which are reduced in PCa and have been shown to regulate VEGF in other cancer types (Szczyrba et al 2010, Fan et al 2012, Yue et al 2012, Boll et al 2013. However, it remains to be determined how effectively these miRNAs repress VEGF translation in PCa.…”

Section: Post-transcriptional Regulation Of Vegf Signaling In Pcamentioning

confidence: 99%

Regulation of vascular endothelial growth factor in prostate cancer

Brot¹,

Ntekim²,

Cardenas³

et al. 2015

Endocrine-Related Cancer

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Prostate cancer (PCa) is the most common malignancy affecting men in the western world. Although radical prostatectomy and radiation therapy can successfully treat PCa in the majority of patients, up to w30% will experience local recurrence or metastatic disease. Prostate carcinogenesis and progression is typically an androgen-dependent process. For this reason, therapies for recurrent PCa target androgen biosynthesis and androgen receptor function. Such androgen deprivation therapies (ADT) are effective initially, but the duration of response is typically %24 months. Although ADT and taxane-based chemotherapy have delivered survival benefits, metastatic PCa remains incurable. Therefore, it is essential to establish the cellular and molecular mechanisms that enable localized PCas to invade and disseminate. It has long been accepted that metastases require angiogenesis. In the present review, we examine the essential role for angiogenesis in PCa metastases, and we focus in particular on the current understanding of the regulation of vascular endothelial growth factor (VEGF) in localized and metastatic PCa. We highlight recent advances in understanding the role of VEGF in regulating the interaction of cancer cells with tumor-associated immune cells during the metastatic process of PCa. We summarize the established mechanisms of transcriptional and post-transcriptional regulation of VEGF in PCa cells and outline the molecular insights obtained from preclinical animal models of PCa. Finally, we summarize the current state of anti-angiogenesis therapies for PCa and consider how existing therapies impact VEGF signaling.

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Section: Post-transcriptional Regulation Of Vegf Signaling In Pcamentioning

confidence: 99%

Regulation of vascular endothelial growth factor in prostate cancer

Brot¹,

Ntekim²,

Cardenas³

et al. 2015

Endocrine-Related Cancer

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“…Yue et al (2012) reported that miR-205 is a potential tumor suppressor in human glioblastoma cells by down-regulating the expression of VEGF-A via direct interactions with the putative miRNA-205 binding site at the 3′UTR site. As another mechanism of tumor suppression by miR-205, Dar et al (2011) reported that miR-205 suppressed cell proliferation by regulating the E2F1 protein in melanoma cells.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-205 Directly Regulates the Tumor Suppressor, Interleukin-24, in Human KB Oral Cancer Cells

Kim

Lee

et al. 2013

Molecules and Cells

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*MicroRNA (miRNA) is a form of small noncoding RNA that regulates the expression of genes either by inhibiting mRNA translation or by inducing its degradation. Small microRNA play important roles in regulating a large number of cellular processes, including development, proliferation and apoptosis. This study examined the biological functions of miR-205 as a tumor suppressor in KB oral cancer cells. The results showed that miR-205 expression was significantly lower in KB oral cancer cells than in human normal oral keratinocytes. Furthermore, the miR-205 over-expressed in KB oral cancer cells increased the cell cytotoxicity and induced apoptosis through the activation of caspase-3/-7. The transfection of miR-205 into KB oral cancer cells strongly induced IL-24, a well known cytokine that acts as a tumor suppressor in a range of tumor tissues. In addition, miR-205 targeted the IL-24 promoter directly to induce gene expression. Overall, miR-205 has significant therapeutic potential to turn on silenced tumor suppressor genes by targeting them with miRNA.

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“…10,17,25,28 In previous observations, we found that miR-205 was significantly downregulated in glioma cell lines and tissue specimens and that ectopic expression of miRNA-205 induces apoptosis and cell-cycle arrest and impairs cell viability, clonability, and invasive properties of glioma cells by directly targeting vascular endothelial growth factor A. 26 In addition, another group found that downregulation of miR-205 in tissues was associated with glioma progression. 9 As shown in the studies discussed above, miR-205 is associated with the occurrence and development of cancers.…”

mentioning

confidence: 97%

“…5,22 Based on our previous observations, microRNA 205 (miR-205) is significantly downregulated in glioma cell lines and tissue specimens. 26 Here, we investigated the diagnostic and prognostic potential of miR-205 in the serum of patients with glioma.…”

mentioning

confidence: 99%

Downregulation of serum microRNA-205 as a potential diagnostic and prognostic biomarker for human glioma

Xiao

Lan

et al. 2016

JNS

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G lioma is the most common primary intracranial tumor in adults, accounting for more than 40% of primary CNS neoplasms. In accordance with WHO classification, which is based on histomorphological criteria, gliomas are categorized as well-differentiated low-grade astrocytoma (WHO Grade I or II), anaplastic astrocytoma (WHO Grade III), or glioblastoma (GBM; WHO Grade IV). 15 The most frequent and malignant glioabbreviatioNs GBM = glioblastoma; KPS = Karnofsky Performance Scale; miR-205 = microRNA 205; miRNA = microRNA; OS = overall survival; PCNSL = primary diffuse large B-cell lymphoma of the CNS; qRT-PCR = quantitative reverse-transcription polymerase chain reaction. obJective Circulating microRNAs (miRNAs) are a new class of highly promising cancer biomarkers. Malignant glioma is one of the most devastating and lethal forms of intrinsic CNS tumor. Here, the authors evaluated serum miRNA 205 (miR-205) levels in patients with glioma. methods Sixty-four patients in whom glioma was diagnosed and 45 healthy controls were recruited between October 2011 and March 2012 and randomly assigned to the screening cohort or the validation cohort. Cohorts of patients with other brain tumors, including meningioma (n = 8), primary diffuse large B-cell lymphoma of the CNS (n = 6), and pituitary adenoma (n = 5), were investigated and compared. miR-205 extraction from serum was detected by real-time quantitative reverse-transcription polymerase chain reaction. The Kaplan-Meier method was applied to perform survival analysis, the risk factors were analyzed by using a Cox regression model, and the receiver operating characteristic working curve was used to analyze the value of miR-205 in the prognostic evaluation of the patients. results The authors first demonstrated that serum miR-205 expression was significantly lower in patients with glioma than in healthy controls (p < 0.001). It is important to note that serum miR-205 expression demonstrated a stepwise decrease with ascending pathological grades. The serum miR-205 biomarker had high sensitivity, specificity, and accuracy in patients with glioma. Serum levels of miR-205 were identified as an individual diagnostic marker and were significantly lower in the glioma cohort than in the other brain tumor cohorts. Serum miR-205 levels were significantly increased in postoperative samples over those in the preoperative samples and were reduced again during glioblastoma recurrences. Statistical analysis revealed a significant correlation between low serum miR-205 expression and both ascending pathological grades (p = 0.002) and low Karnofsky Performance Scale scores (p = 0.01). Patients with glioma at an advanced pathological grade (Grade III or IV) and a higher miR-205 serum level showed longer overall survival than those with a lower miR-205 serum concentration (p < 0.01). Furthermore, Cox regression analysis revealed that miR-205 serum levels were independently associated with overall survival. coNclusioNs These data indicate that serum miR-205 expression is a novel and valuable bioma...

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MicroRNA-205 functions as a tumor suppressor in human glioblastoma cells by targeting VEGF-A

Cited by 91 publications

References 35 publications

Regulation of vascular endothelial growth factor in prostate cancer

Regulation of vascular endothelial growth factor in prostate cancer

MicroRNA-205 Directly Regulates the Tumor Suppressor, Interleukin-24, in Human KB Oral Cancer Cells

Downregulation of serum microRNA-205 as a potential diagnostic and prognostic biomarker for human glioma

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