MicroRNA‐205–directed transcriptional activation of tumor suppressor genes in prostate cancer

Majid, Shahana; Dar, Altaf A.; Saini, Sharanjot; Yamamura, Soichiro; Hirata, Hiroshi; Tanaka, Yuichiro; Deng, Guoren; Dahiya, Rajvir

doi:10.1002/cncr.25488

Cited by 259 publications

(197 citation statements)

References 41 publications

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“…Here, we used qRT-PCR to profile the expression of miR-205 in PCa and non-cancerous tissues. Consistent with previous studies, 16,17 we demonstrated that the expression of miR-205 in PCa tissues was down-regulated when compared with non-cancerous tissues (Figure 1b). Furthermore, miR-205 was shown to be downregulated in advanced-stage PCa in our studies (Figure 1a and 1b) and to be down-regulated in metastatic PCa.…”

Section: Mir-205 Suppresses Tumor Growth In Vivosupporting

confidence: 92%

“…[11][12][13][14][15] In addition, miR-205 has been shown to activate IL24 and IL32 by targeting sites on their promoters. 16 In PCa, miR-205 exerts a tumorsuppressive effect by counteracting the epithelial-to-mesenchymal transition and reducing cell migration/invasion, in part through the down-regulation of protein kinase C epsilon. 17 In this study, we analysed miR-205 expression in advanced and early PCa tissues among Chinese patients using a quantitative real-time PCR assay.…”

Section: Introductionmentioning

confidence: 99%

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miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth

Wang¹,

Li²,

Feng³

et al. 2013

Asian J Androl

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The purpose of this study was to elucidate the molecular mechanisms of microRNA-205 (miR-205) as a tumor suppressor in prostate cancer (PCa). In the present study, microRNA microarray analysis suggested that the expression of miR-205 was significantly decreased in advanced PCa compared with early PCa. Real-time PCR analysis also indicated that miR-205 expression was significantly decreased in PCa tissues compared with non-cancerous tissues. Moreover, the expression of miR-205 has been demonstrated to be associated with the clinicopathological stage and total/free prostate-specific antigen (PSA) level of PCa. Functional analyses showed that both the overexpression of miR-205 and the knockdown of c-SRC in PCa cell lines could inhibit cell growth, colony formation, migration, invasion and the cell cycle as well as induce cell apoptosis in vitro. Furthermore, over-expressing miR-205 reduced tumorigenicity in vivo. Through a luciferase activity assay and Western blotting, c-SRC was identified as a target of miR-205 in cells. The overexpression of miR-205 suppressed c-SRC and its downstream signaling molecules, including FAK, p-FAK, ERK1/2 and p-ERK1/2, and attenuated cell proliferation, invasion and tumor growth.

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Section: Mir-205 Suppresses Tumor Growth In Vivosupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth

Wang¹,

Li²,

Feng³

et al. 2013

Asian J Androl

View full text Add to dashboard Cite

show abstract

“…Additional targets of miR-205 identified in other tissue types have not been validated in breast cancer. [83][84][85][86] Likely, miR-205 plays a role in specific pathways in tumorigenesis and tumor progression in a cell type-specific manner.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA expression profiling identifies decreased expression of miR-205 in inflammatory breast cancer

et al. 2016

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Inflammatory breast cancer is the most aggressive form of breast cancer. Identifying new biomarkers to be used as therapeutic targets is in urgent need. Messenger RNA expression profiling studies have indicated that inflammatory breast cancer is a transcriptionally heterogeneous disease, and specific molecular targets for inflammatory breast cancer have not been well established. We performed microRNA expression profiling in inflammatory breast cancer in comparison with locally advanced noninflammatory breast cancer in this study. Although many microRNAs were differentially expressed between normal breast tissue and tumor tissue, most of them did not show differential expression between inflammatory and noninflammatory tumor samples. However, by microarray analysis, quantitative reverse transcription PCR, and in situ hybridization, we showed that microRNA-205 expression was decreased not only in tumor compared with normal breast tissue, but also in inflammatory breast cancer compared with noninflammatory breast cancer. Lower expression of microRNA-205 correlated with worse distant metastasis-free survival and overall survival in our cohort. A small-scale immunohistochemistry analysis showed coexistence of decreased microRNA-205 expression and decreased E-cadherin expression in some ductal tumors. MicroRNA-205 may serve as a therapeutic target in advanced breast cancer including inflammatory breast cancer.

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“…This miRNA was the most strongly downregulated miRNA in PCa compared with NAP and its expression is completely abolished in metastatic lymph node and malignant TURP samples. It has been suggested that the tumorsuppressive function of miR-205 takes place through counteracting epithelial-to-mesenchymal transition and reducing cell migration and invasion in the human prostate (Gandellini et al, 2009;Majid et al, 2010). K27me3 modifications of the miR-205 locus was reported to occur in the PCa cell line PC3 (Ke et al, 2009) and in muscle-invasive bladder tumors and undifferentiated bladder cell lines (Wiklund et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

et al. 2011

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Prostate cancer (PCa) is the most frequent male malignancy and the second most common cause of cancer-related death in Western countries. Current clinical and pathological methods are limited in the prediction of postoperative outcome. It is becoming increasingly evident that small non-coding RNA (ncRNA) species are associated with the development and progression of this malignancy. To assess the diversity and abundance of small ncRNAs in PCa, we analyzed the composition of the entire small transcriptome by Illumina/ Solexa deep sequencing. We further analyzed the micro-RNA (miRNA) expression signatures of 102 fresh-frozen patient samples during PCa progression by miRNA microarrays. Both platforms were cross-validated by quantitative reverse transcriptase-PCR. Besides the altered expression of several miRNAs, our deep sequencing analyses revealed strong differential expression of small nucleolar RNAs (snoRNAs) and transfer RNAs (tRNAs). From microarray analysis, we derived a miRNA diagnostic classifier that accurately distinguishes normal from cancer samples. Furthermore, we were able to construct a PCa prognostic predictor that independently forecasts postoperative outcome. Importantly, the majority of miRNAs included in the predictor also exhibit high sequence counts and concordant differential expression in Illumina PCa samples, supported by quantitative reverse transcriptase-PCR. Our findings provide miRNA expression signatures that may serve as an accurate tool for the diagnosis and prognosis of PCa.

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MicroRNA‐205–directed transcriptional activation of tumor suppressor genes in prostate cancer

Cited by 259 publications

References 41 publications

miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth

miR-205 is frequently downregulated in prostate cancer and acts as a tumor suppressor by inhibiting tumor growth

MicroRNA expression profiling identifies decreased expression of miR-205 in inflammatory breast cancer

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

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