microRNA-204-5p participates in atherosclerosis via targeting MMP-9

Wang, Na; Yuan, Yuliang; Sun, Shipeng; Liu, Guijian

doi:10.1515/med-2020-0034

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…Reportedly, in atherosclerotic plaque tissue and blood samples, the expression level of miR-204-5p was markedly downregulated. Moreover, the overexpression of miR-204-5p may also reduce human vascular smooth muscle cell (hVSMCs) proliferation and migration while increasing hVSMC apoptosis [ 11 ]. Another study confirmed that miR-204 was downregulated in hypoxic-ischemic encephalopathy (HIE) [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Qiao

Guan

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. To determine the level of expression and clinical importance of serum miR-204 and miR-451 in patients with pulmonary arterial hypertension caused by congenital heart disease (CHD-PAH). Methods. From July 2019 to January 2021, 114 infants with congenital heart disease (CHD) were hospitalized at Qingdao’s Fuwai Cardiovascular Hospital. They were grouped into categories: CHD (53 cases) and CHD-PAH (61 cases) based on whether they had pulmonary hypertension (PAH). In addition, 60 healthy children were selected as the control group. All children underwent routine biochemical examination, echocardiography, and pulmonary arterial pressure examination. By using an enzyme-linked immunosorbent assay (ELISA), the levels of brain natriuretic peptide (BNP) and asymmetric dimethylarginine (ADMA) in the blood were measured. Additionally, reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression levels of miR-204 and miR-451 in peripheral blood. The correlation between miR-204, miR-451, BNP, ADMA, and mPAP was investigated using Pearson correlation analysis. Results. Consequently, the TC, BNP, and ADMA serum levels were considerably higher in the CHD and CHD-PAH groups than in the control group ( P < 0.05 ), whereas BNP and ADMA serum levels were significantly higher in the CHD-PAH group than in the CHD group ( P < 0.05 ). According to RT-PCR data, the expression levels of miR-204 and miR-451 in the peripheral blood of children in the CHD and CHD-PAH groups were significantly lower ( P < 0.05 ) when compared to the control group. The expression levels of miR-204 and miR-451 in the peripheral blood of children in the CHD-PAH group were substantially lower ( P < 0.05 ) than in the CHD group. Significantly, the findings of the ROC curve revealed that the area under the curve (AUC) of CHD-PAH diagnosed by miR-204 and miR-451 alone was 0.737 and 0.725, respectively, and the AUC of joint diagnosis was 0.840, which was greater than that of single diagnosis ( P < 0.05 ). Patients with CHD-PAH had lower levels of miR-204 and miR-451 in their blood, and this was found to be associated with BNP, ADMA, and mPAP, analyzed by Pearson correlation. Conclusions. Children with CHD-PAH can be diagnosed based on aberrant expressions of miR-204 and miR-451 in their peripheral blood serum. Moreover, CHD-PAH can be diagnosed if the combination of miR-204 and miR-451 is detected as a biomarker, which has a higher diagnostic value.

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Section: Introductionmentioning

confidence: 99%

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Qiao

Guan

et al. 2022

Computational and Mathematical Methods in Medicine

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“…Furthermore, we demonstrated that miR-204 mediates the atheroprotective effects of NFATc3 in ApoE À/À mice (Liu et al, 2021). These were the main reasons for using the ApoE À/À (Liu et al, 2021;Wang, Yuan, et al, 2020), sera of diabetic patients (Wang, Ding, et al, 2020), and calcified artery tissues of patients with uremia (Lin et al, 2018). Notably, the cytoplasmic levels of mature miR-204-3p remained unchanged upon CsA treatment, whereas cytoplasmic pre-miR-204 levels were reduced.…”

Section: Discussionmentioning

confidence: 94%

Dual action of macrophage miR‐204 confines cyclosporine A‐induced atherosclerosis

Su,

Hong,

Han

et al. 2023

British J Pharmacology

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Background and PurposeAtherosclerosis induced by cyclosporine A (CsA), an inhibitor of the calcineurin/nuclear factor of activated T cells (NFAT) pathway, is a major concern after organ transplantation. However, the atherosclerotic mechanisms of CsA remain obscure. We previously demonstrated that calcineurin/NFAT signalling inhibition contributes to atherogenesis via suppressing microRNA‐204 (miR‐204) transcription. We therefore hypothesised that miR‐204 is involved in the development of CsA‐induced atherosclerosis.Experimental ApproachApoE−/− mice with macrophage‐miR‐204 overexpression were generated to determine the effects of miR‐204 on CsA‐induced atherosclerosis. Luciferase reporter assays and chromatin immunoprecipitation sequencing were performed to explore the targets mediating miR‐204 effects.Key ResultsCsA alone did not significantly affect atherosclerotic lesions or serum lipid levels. However, it exacerbated high‐fat diet‐induced atherosclerosis and hyperlipidemia in C57BL/6J and ApoE−/− mice, respectively. miR‐204 levels decreased in circulating monocytes and plaque lesions during CsA‐induced atherosclerosis. The upregulation of miR‐204 in macrophages inhibited CsA‐induced atherosclerotic plaque formation but did not affect serum lipid levels. miR‐204 limited the CsA‐induced foam cell formation by reducing the expression of the scavenger receptors SR‐BII and CD36. SR‐BII was post‐transcriptionally regulated by mature miR‐204‐5p via 3′‐UTR targeting. Additionally, nuclear‐localised miR‐204‐3p prevented the CsA‐induced binding of Ago2 to the CD36 promoter, suppressing CD36 transcription. SR‐BII or CD36 expression restoration dampened the beneficial effects of miR‐204 on CsA‐induced atherosclerosis.Conclusion and ImplicationsMacrophage miR‐204 ameliorates CsA‐induced atherosclerosis, suggesting that miR‐204 may be a potential target for the prevention and treatment of CsA‐related atherosclerotic side effects.

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“…MMP-9 is regarded as a new effective marker and a possible therapeutic target for diseases based on atherosclerosis. 30,31 In addition, MMP-9 also has potential as an indicator of the early stages of atherosclerotic damage, since an increase in the level of this substance most likely evidences destabilization of the atherosclerotic plaque and destruction of myocardial tissue, and also MMP-9 shows a signifi cant prognostic value. 14 MMP-9 levels increased in patients with acute myocardial infarction with ST-segment elevation and MMP-9 appeared to be a signifi cant biomarker for the diagnosis of acute myocardial infarction with ST-segment elevation.…”

Section: Discussionmentioning

confidence: 99%

Matrix metallopeptidase-9 prognostic role in STEMI patients after percutaneous coronary intervention (PCI) in one-year follow-up period

Kopytsya¹,

Нilоva²,

Rodionova³

et al. 2023

Cor Vasa

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Použití matrixové metalopeptidázy-9 (MMP-9) u pacientů s akutním infarktem myokardu s elevacemi úseku ST (STEMI) po perkutánní koronární intervenci (PCI) jako prognostického indikátoru má mnoho mezer a lékařská komunita s tím má velmi omezené zkušenosti. Cílem studie bylo zhodnotit prognostickou roli MMP-9 u pacientů se STEMI po úspěšné revaskularizaci během 12měsíčního období sledování. Materiál a metoda: Do studie bylo zařazeno 88 pacientů, kteří splnili kritéria pro zařazení a neměli žádná vylučovací kritéria, jimž byl diagnostikován STEMI a podstoupili primární PCI. Mužů bylo 66 (75,0 %), s průměrným věkem 60,84 ± 9,68 roku. Doba sledování činila 12 měsíců. Byl použit složený cílový ukazatel, který zahrnoval výskyt srdečního selhání, které si vyžádalo hospitalizaci, úmrtí z kardiovaskulárních příčin, recidivující infarkt myokardu, akutní cerebrovaskulární příhodu. Koncentrace MMP-9 byla stanovena pomocí ELISA. Výsledky: Kaplanova-Meierova analýza křivek ukázala významnou souvislost mezi zvýšenou MMP-9 a studovaným složeným cílovým ukazatelem, p = 0,0229. Pacienti s koncentracemi MMP-9 > 201,7 ng/ml měli signifi kantně více cílových ukazatelů. Jednorozměrná regresní analýza zjistila, že MMP-9 byla indikována jako nezávislá proměnná pro nepříznivý výsledek s poměrem šancí (OR):

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microRNA-204-5p participates in atherosclerosis via targeting MMP-9

Cited by 14 publications

References 26 publications

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Serum miR-204 and miR-451 Expression and Diagnostic Value in Patients with Pulmonary Artery Hypertension Triggered by Congenital Heart Disease

Dual action of macrophage miR‐204 confines cyclosporine A‐induced atherosclerosis

Matrix metallopeptidase-9 prognostic role in STEMI patients after percutaneous coronary intervention (PCI) in one-year follow-up period

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