MicroRNA-203 regulates caveolin-1 in breast tissue during caloric restriction

Abstract

Caloric restriction has been shown to increase lifespan in several organisms and to delay onset of age-related diseases. The transcriptional response to caloric restriction has been studied for mRnAs, while the microRnA signature following caloric restriction remains unexplored. here, we characterize the microRnA expression in mouse breast tissue before and after caloric restriction, reporting several changes in the microRnA expression profile. In particular, miR-203 is found to be highly induced by caloric restriction, and we demonstrate that caveolin-1 as well as p63 are direct targets of miR-203 in vivo during caloric restriction. Using tissue culture models, we suggest that this regulation is important in both mouse and human. In conclusion, we show that the microRnA response induced by caloric restriction can regulate important factors in processes such as longevity and aging and is an integral and important component of the cellular response to caloric restriction.Caloric restriction (CR) has been shown to have a positive effect on life span across several organisms [1][2][3] and is speculated to be beneficial for the treatment of age-related diseases. Delineating the genetic response to CR is crucial to understand these effects on aging and disease and specifically in breast tissue since CR has been shown to have potential therapeutic benefit in diseases of the breast. 4,5

Lipid rafts in immune signalling: current progress and future perspective

“…showed that miR‐133a attenuates metastasis in head and neck squamous cell carcinoma cells by targeting caveolin‐1. Other studies from our laboratory and other groups showed that microRNAs (miR‐195, miR‐203, miR‐199a‐3p and miR‐218) significantly inhibit migration and invasion in cancer cells by targeting caveolins . In an independent study, Li et al .…”

MicroRNAs Mediate Dietary-Restriction-Induced Longevity through PHA-4/FOXA and SKN-1/Nrf Transcription Factors

“…Our findings demonstrate a role for miRNAs in dietary restriction of C. elegans , but given the conservation of miRNAs and PHA-4 and SKN-1 across species, these interactions may also be conserved in higher species. A few studies have reported numerous changes in the mammalian miRNA expression profile under dietary restriction [56–58]. Thus, it may also hold true that the miRNAs induced upon dietary restriction in mammals may be responsible for transducing the effects of lifespan extension in higher species, as well.…”

Caveolin-1 is transcribed from a hypermethylated promoter to mediate colonocyte differentiation and apoptosis

“…These results suggested that CAV1 induction by butyrate was transcriptionally regulated. Number of recent studies have shown that microRNAs, such as miR-802 [34] miR-199a-5p [35] and miR-203 [36] directly suppress the expression of CAV1 by binding to its 3 0 -UTR region. Butyrate may also alter miRNA expression in the cells [24].…”