MicroRNA-202 (miR-202) controls female fecundity by regulating medaka oogenesis

Abstract

Female gamete production relies on coordinated molecular and cellular processes that occur in the ovary throughout oogenesis. In fish, as in other vertebrates, these processes have been extensively studied both in terms of endocrine/paracrine regulation and protein expression and activity. The role of small non-coding RNAs in the regulation of animal reproduction remains however largely unknown and poorly investigated, despite a growing interest for the importance of miRNAs in a wide variety of biological processes. Here, we analyzed the role of miR-202, a miRNA predominantly expressed in male and female gonads in several vertebrate species. We studied its expression in the medaka ovary and generated a mutant line (using CRISPR/Cas9 genome engineering) to determine its importance for reproductive success with special interest for egg production. Our results show that miR-202-5p is the biologically active form of the miRNA and that it is expressed in granulosa cells and in the unfertilized egg. The knock out (KO) of miR-202 resulted in a strong phenotype both in terms of number and quality of eggs produced. Mutant females exhibited either no egg production or produced a drastically reduced number of eggs that could not be fertilized, ultimately leading to no reproductive success. We quantified the size distribution of the oocytes in the ovary of KO females and performed a genome-wide transcriptomic analysis approach to identified dysregulated molecular pathways. Together, cellular and molecular analyses indicate that lack of miR-202 impairs the early steps of oogenesis/folliculogenesis and decreases the number of large (i.e. vitellogenic) follicles, ultimately leading to dramatically reduced female fecundity. This study sheds new light on the regulatory mechanisms that control the early steps of follicular development and provides the first in vivo functional evidence that an ovarian-predominant microRNA may have a major role in female reproduction.Author summaryThe role of small non-coding RNAs in the regulation of animal reproduction remains poorly investigated, despite a growing interest for the importance of miRNAs in a wide variety of biological processes. Here, we analyzed the role of miR-202, a miRNA predominantly expressed in gonads in vertebrate. We studied its expression in the medaka ovary and knocked out the miR-202 genes to study its importance for reproductive success. We showed that the lack of miR-202 results in the sterility of both females and males. In particular, it lead to a drastic reduction of both the number and the quality of eggs produced by females. Mutant females exhibited either no egg production or produced a drastically reduced number of eggs that could not be fertilized, ultimately leading to no reproductive success. Quantitative histological and molecular analyses indicated that miR-202 KO impairs oocyte development and is also associated with the dysregulation of many genes that are critical for reproduction. This study sheds new light on the regulatory mechanisms that control oogenesis and provides the first in vivo functional evidence that an ovarian-predominant microRNA may have a major role in female reproduction.

miRNA analysis with Prost! reveals evolutionary conservation of organ-enriched expression and post-transcriptional modifications in three-spined stickleback and zebrafish

“…For example, miR429a-3p was enriched in testis in stickleback but enriched in ovary in zebrafish; miR10c-5p was enriched in ovary in stickleback but enriched in testis in zebrafish; miR204-5p was enriched in ovary in zebrafish but enriched in both gonads in stickleback; miR196a-5p was enriched in testis in zebrafish and in both gonads in stickleback (Figure 4G); miR19c-3p, miR194a-5p, and miR725-3p were enriched in testis in stickleback but enriched in both gonads in zebrafish. In the case of the well-known gonad-specific miR202-5p 80–82 , the expression level in the stickleback ovary was significantly higher than in testis; although the trend was the same in zebrafish, the difference was not statistically significant (Figure 4H).…”

An end-to-end pipeline based on open source deep learning tools for reliable analysis of complex 3D images of Medaka ovaries

“…One of the greatest challenges facing research on the development of ovarian dynamics and functions is the lack of an effective method to accurately count growing oocytes regardless of their stage. Studies have indeed traditionally been limited to automatic or manual oocyte counting on two-dimensional (2D) ovarian sections and extrapolation of the data to the whole organ or to manual counting of dissociated follicles (Gay et al, 2018; Iwamatsu, Takashi, 1978; Iwamatsu, 2015). Some studies have also focused on the development of complex stereological approaches to limit the biases induced by 2D approaches (Charleston et al, 2007).…”