microRNA‑200a functions as a tumor suppressor by targeting FOXA1 in glioma

Abstract

microRNAs (miRs) serve primary roles in certain human malignancies; however, the detailed regulatory mechanism of miR-200a in glioma progression is yet to be fully elucidated. The current study aimed to assess the expression of miR-200a in glioma as well as the regulatory mechanism of miR-200a in glioma cell proliferation, survival and invasion. RT-qPCR and western blotting were performed to examine mRNA and protein expression. An MTT assay, an EdU incorporation cell proliferation assay and a transwell assay were utilized to assess cell survival, proliferation and invasion. The results indicated that the miR-200a levels were significantly reduced in glioma tissues compared with normal brain tissues. Levels were also downregulated in glioma cell lines when compared with those in normal human astrocyte cells. Furthermore, low miR-200a expression was associated with advanced progression of glioma. The overexpression of miR-200a inhibited glioma cell proliferation, survival and invasion. Results also identified that FOXA1 was a target gene of miR-200a in glioma cells and that the increased expression of FOXA1 was negatively correlated to the decreased expression of miR-200a in glioma tissues. Furthermore, FOXA1 expression was negatively mediated by miR-200a in glioma cells and the overexpression of FOXA1 eliminated the inhibitory effects of miR-200a on the survival, proliferation and invasion of glioma cells. In conclusion, the current study demonstrated that miR-200a functions acts as a tumor suppressor in glioma by directly targeting FOXA1 and may thus be a potential candidate for the treatment of glioma.

Integrated Analysis Identifies a Nine-microRNA Signature Biomarker for Diagnosis and Prognosis in Colorectal Cancer

“…The miR-200 family (miR-200a, -200b, -200c, -141, and -429) has been demonstrated as an inhibitor of epithelial-tomesenchymal transition (EMT) and metastasis of cancer in colon (Hur et al, 2013). Chen et al (2019) reported that miR-200a is a tumor suppressor in glioma by directly targeting forkhead box A1 (FOXA1). FOXA1 is a transcription factor that is essential for epithelial lineage differentiation, playing an oncogenic or tumor-suppressive role in human malignancies (Song et al, 2019).…”

Competitive endogenous RNA (ceRNA) regulation network of lncRNAs, miRNAs, and mRNAs in Wilms tumour

“…miR-200a is an important member of the miR-200 family. It has been reported that miR-200a participates in several biological processes to control the progression of cancer [26,27]. miR-200a was shown to target FOXA1 and act as a tumour suppressor in the survival, proliferation and invasion of glioma cells [27].…”

“…It has been reported that miR-200a participates in several biological processes to control the progression of cancer [26,27]. miR-200a was shown to target FOXA1 and act as a tumour suppressor in the survival, proliferation and invasion of glioma cells [27]. In addition, miR-200a might inactivate BRD4-mediated AR signalling to inhibit the progression of prostate cancer [26].…”

Competitive Endogenous RNA (ceRNA) Regulation Network of lncRNA–miRNA–mRNA in Wilms tumor

“…MiR-200a was an important member of miR-200 family. It had been reported that miR-200a was involved in several biological processes to control progression of cancer [27,28]. MiR-200a was showed to target FOXA1 and acts as a tumor suppressor on the survival, proliferation and invasion of glioma cells [28].…”

“…It had been reported that miR-200a was involved in several biological processes to control progression of cancer [27,28]. MiR-200a was showed to target FOXA1 and acts as a tumor suppressor on the survival, proliferation and invasion of glioma cells [28]. And miR-200a might inactivate BRD4-mediated AR signaling to inhibit progression of prostate cancer [27].…”