MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma

Won, Kyu Yeoun; Kim, Youn Wha; Kim, Hyun Sook; Lee, Seung Kwan; Jung, Woon Won; Park, Yong Koo

doi:10.1016/j.humpath.2013.01.016

Cited by 65 publications

(55 citation statements)

References 34 publications

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“…Our findings supported that miR-150 expression is downregulated in OS MG63 cells, which is consistent with previous studies in OS. Nevertheless, several researchers have found that miR-150 may be upregulated in OS samples when compared to normal osteoblasts [Lulla et al, 2011;Won et al, 2013], which was contrary to the findings in our study. Considering this conflicting results, it is very important to conduct further studies to evaluate the effect of miR-150 on the development and progression of OS.…”

Section: Discussioncontrasting

confidence: 99%

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Xiao

Chen²,

Wang³

et al. 2015

Cytogenet Genome Res

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miR-150 expression in osteosarcoma (OS) cell lines and human osteoblast cells was detected, and OS cell models were transfected with exogenous miR-150 to investigate its role in cell proliferation, invasion, and apoptosis. Our results showed that miR-150 expression in OS cells (MG63, Saos-2, SOSP-9607, and U2OS) was significantly lower compared to the osteoblast hFOB1.19 cell line (all p < 0.01). The expression level of miR-150 in MG63 cells that were transfected with exogenous miR-150 mimics was markedly upregulated, while the miR-150 expression level in the inhibitor group was significantly downregulated (both p < 0.01). Similar results were also found in SOSP-9607 cells. Importantly, exogenous miR-150 expression stimulated cell apoptosis and inhibited proliferation, invasion, and migration. A luciferase reporter assay displayed that miR-150 also regulated Sp1 expression by targeting its 3′-UTR, and qRT-PCR and Western blotting showed that elevated levels of miR-150 may reduce Sp1 protein expression. The mRNA and protein levels of Sp1 were upregulated after transfection with a Sp1-expression plasmid and partially reversed the inhibitory effects of miR-150 on cell proliferation, invasion, and metastasis in MG63 and SOSP-9607 cells, as well as promoted cell apoptosis. In conclusion, miR-150 inhibits cell proliferation, invasion, and metastasis and stimulates cell apoptosis by regulating the expression of Sp1. Therefore, miR-150 may be a potential clinical target for the treatment of OS patients.

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Section: Discussioncontrasting

confidence: 99%

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Xiao

Chen²,

Wang³

et al. 2015

Cytogenet Genome Res

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“…The mRNA expression level of Ezrin was significantly decreased in F5M2 osteosarcoma cells that transfected with high dose of miR-144 mimics (high miR-144 group) Fig. 4 Transwell experiments of the migration and invasion of F5M2 osteosarcoma cells in F5M2 group, F5M2 NC group, low miR-144 group, high miR-144 group, and the inhibitor group, respectively play an essential role in many aspects of cell behavior including development, proliferation, apoptosis, and differentiation; thus, it is a new research area to search for better treatment options for osteosarcoma patients [31,33]. In this study, we investigated the potential role and underlying mechanisms of miR-144 in the invasion and metastasis process of osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-144 inhibits the proliferation, apoptosis, invasion, and migration of osteosarcoma cell line F5M2

Cui

Wang

2015

Tumor Biol.

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This study is aimed to investigate the role of microRNA-144 (miR-144) in osteosarcoma cell line F5M2 proliferation, apoptosis, invasion, and metastasis. Between 2007 and 2014, 66 cases of osteosarcoma samples in the corresponding adjacent normal tissue samples were selected from surgical resection or biopsy in the Department of Orthopedics, Shengjing Hospital, China Medical University. MiR-144 levels and Ezrin messenger RNA (mRNA) levels in osteosarcoma and the adjacent bone tissues were detected, and clinical and pathological features were analyzed. Exogenous miR-144 was transfected into human osteosarcoma cell lines at two different concentrations (low and high), and the expression levels of miR-144 and Ezrin protein between highly metastatic osteosarcoma cells and lowly metastatic osteosarcoma cells were compared. Real-time polymerase chain reaction (RT-PCR) and Western blot were used for detecting the expression levels of miR-144 or Ezrin protein, respectively. Cell proliferation was measured by methylthiazol tetrazolium (MTT) assay. Cell invasion and migration was evaluated by Transwell assays. Finally, flow cytometry was employed to determine the cell apoptosis. MiR-144 expression in osteosarcoma tissue was significantly lower than that in the surrounding normal bone tissue (P < 0.001), while Ezrin mRNA expression in osteosarcoma tissue was significantly higher than that in the surrounding normal bone tissue (P < 0.001); correlation analysis showed a significant negative correlation between miR-144 and Ezrin mRNA levels (r = 0.982, P < 0.001). MiR-144 and Ezrin mRNA expressions were significantly related with cell metastasis (P < 0.05) but were not related with other clinical factors such as gender, age, tumor location, tumor size, Enneking staging, and Dahlin's histological classification. The results of RT-PCR showed that the expression level of miR-144 in osteosarcoma cells increased after transfected with exogenous miR-144 mimics, and this increase positively correlated with the transfection concentration of miR-144 mimics. Furthermore, Western blotting revealed a significant and dose-dependent decrease in Ezrin protein levels in F5M2 cells transfected with miR-144. MTT and Transwell assays showed that the invasion and metastasis of F5M2 cells was significantly decreased following exogenous overexpression of miR-144. Our study supports the view that the miR-144 may regulate Ezrin protein expression by inhibiting the invasion and metastasis of osteosarcoma cells.

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“…Let-7f effects on thyroid growth and differentiation were reported to attenuate the neoplastic process of RET/PTC papillary thyroid oncogenesis through impairment of MAPK (Rossing et al 2012). miR-199b-5p targets HER2 in breast cancer cells (Fang et al 2013) and is involved in the Notch signaling pathway in osteosarcoma (Won et al 2013).…”

Section: Downregulation Of Specific Micrornasmentioning

confidence: 99%

Cancer stem-like cells and thyroid cancer

Guo¹,

Hardin²,

Lloyd³

2014

Endocrine Related Cancer

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Thyroid cancer is one of the most rapidly increasing malignancies. The reasons for this increase is not completely known, but increases in the diagnosis of papillary thyroid microcarcinomas and follicular variant of papillary thyroid carcinomas along with the enhanced detection of well-differentiated thyroid carcinomas are probably all contributing factors. Although most cases of well-differentiated thyroid carcinomas are associated with an excellent prognosis, a small percentage of patients with well-differentiated thyroid carcinomas as well as most patients with poorly differentiated and anaplastic thyroid carcinomas have recurrent and/or metastatic disease that is often fatal. The cancer stem-like cell (CSC) model suggests that a small number of cells within a cancer, known as CSCs, are responsible for resistance to chemotherapy and radiation therapy, as well as for recurrent and metastatic disease. This review discusses current studies about thyroid CSCs, the processes of epithelial-to-mesenchymal transition (EMT), and mesenchymal-to-epithelial transition that provide plasticity to CSC growth, in addition to the role of microRNAs in CSC development and regulation. Understanding the biology of CSCs, EMT and the metastatic cascade should lead to the design of more rational targeted therapies for highly aggressive and fatal thyroid cancers.

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MicroRNA-199b-5p is involved in the Notch signaling pathway in osteosarcoma

Cited by 65 publications

References 34 publications

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

MicroRNA-144 inhibits the proliferation, apoptosis, invasion, and migration of osteosarcoma cell line F5M2

Cancer stem-like cells and thyroid cancer

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