MicroRNA-196a as a Potential Diagnostic Biomarker for Esophageal Squamous Cell Carcinoma

Fendereski, Mona; Zia, Mohammad Farid; Shafiee, Mohammad; Safari, Forousan; Saneie, Mohammad Hosien; Tavassoli, Manoochehr

doi:10.1080/07357907.2016.1254228

Cited by 25 publications

(20 citation statements)

References 30 publications

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“…It has also been reported that miR-196a inhibits cell apoptosis, and promotes cell proliferation and invasion by targeting the inhibitor of growth family member 5 in pancreatic cancer (21). Furthermore, Maru et al (18) reported that miR-196a is upregulated in esophageal adenocarcinoma, and Fendereski et al (9) described an overexpression of miR-196a in ESCC. However, to the best of our knowledge, few studies have examined the role of miR-196a in regulation of the ESCC phenotype, and the role of miR-196a in ESCC remains unclear.…”

Section: Discussionmentioning

confidence: 98%

“…It has previously been reported that miRNA dysregulation is involved in the development and progression of cancer (6). Previous studies have revealed that miR-196a is upregulated in various types of cancer, including ESCC (7)(8)(9). Further studies have demonstrated that miR-196a promotes tumor progression and acts as an oncogene in some types of cancer (10,11).…”

Section: Introductionmentioning

confidence: 99%

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miR‑196a regulates the proliferation, invasion and migration of esophageal squamous carcinoma cells by targeting ANXA1

Peng

et al. 2019

Oncol Lett

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MicroRNA (miR)-196a is upregulated in various types of malignancy, including esophageal squamous cell carcinoma (ESCC); however, its role in ESCC is currently unclear. The present study aimed to investigate the biological role and molecular mechanism of miR-196a in ESCC. The expression levels of miR-196a in 25 tumor tissues and adjacent non-tumor tissues from patients with ESCC were measured by reverse transcription-quantitative polymerase chain reaction. In addition, miR-196a expression levels were assessed in the human normal esophageal epithelial cell line Het-1A and the ESCC cell line EC109. The effects of miR-196a on the proliferation, apoptosis, invasion and migration of EC109 cells were determined by MTT, flow cytometry and Transwell assays, respectively. A luciferase reporter assay and western blotting were performed to confirm the target gene of miR-196a, and to explore the molecular mechanism underlying the effects of miR-196a on regulation of ESCC cell phenotypes. The results demonstrated that miR-196a was markedly upregulated in ESCC tissues and EC109 cells. In addition, miR-196a downregulation suppressed EC109 cell proliferation, invasion and migration, but did not affect apoptosis. Annexin A1 (ANXA1) was demonstrated to be a direct target gene of miR-196a. ANXA1 protein knockdown reversed the effects of miR-196a inhibition on EC109 cell proliferation, invasion and migration. Furthermore, alongside the downregulation of miR-196a and the increase in ANXA1 expression, cyclooxygenase 2 (COX2), matrix metalloproteinase (MMP)-2 and Snail were downregulated, and E-cadherin was upregulated in EC109 cells. The results of the present study suggested that miR-196a may act as an oncogene in ESCC, and that miR-196a may regulate the proliferation, invasion and migration of ESCC cells by targeting ANXA1. Subsequently, ANXA1 may further modulate the expression levels of COX2, MMP-2, Snail and E-cadherin. In conclusion, the miR-196a/ANXA1 axis may represent a potential therapeutic target in ESCC.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

miR‑196a regulates the proliferation, invasion and migration of esophageal squamous carcinoma cells by targeting ANXA1

Peng

et al. 2019

Oncol Lett

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“…Correlation of aberrant miR-196a expression and single nucleotide polymorphisms with chemosensitivity and radioresistance has been demonstrated in different studies, suggesting that investigating expression patterns and known polymorphisms might be useful in development of therapeutic strategies (29,30). Upregulation of miR-196a was previously shown in esophageal adenocarcinoma, in saliva specimens and ESCC cell lines (11,12,22). The alteration of miR-196a expression is in concordance with previous studies, but the lack of statistical significance as well as the non-significant relationship between expression changes and short survival could be due to the small sample size in our study.…”

Section: Correlation Analysis Of the Mir-196a With Clinicopathologicamentioning

confidence: 95%

“…Evidence suggests that expression of this microRNA may be altered in esophageal adenocarcinoma cancer cells. Moreover, it's up regulation has been recently discovered in the saliva specimens from ESCC patients and ESCC cell lines (11,12). It seems that the level of miR-196a expression is associated with metastasis, poor prognosis and reduced overall survival of the patients (13,14).…”

Section: Introductionmentioning

confidence: 99%

Expression Analysis of MicroRNA-196a in Esophageal Cancer

Maghsudlu

Yazd

Amiriani

2019

jcbr

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Backgrounds and objectives: Esophageal squamous cell carcinoma (ESCC) is a prevalent subtype of esophageal cancer in certain areas of the world. Molecular evaluations can provide a better understanding of the disease that could contribute to earlier diagnosis and treatment. Amplification or absence of microRNA (miRNA) genes has been described in many types of cancer, indicating that altered patterns of miRNA expression may affect cell cycle and its features. In this study, we investigated alteration of miR-196a expression in ESCC tissues and its association with tumor status. Methods: The study was done on 30 specimens including matched tumor and normal tumor margin tissues. RNA extraction and complementary DNA synthesis were performed using specific primers. Quantitative PCR was done to assess the relative expression of miR-196a in tumor tissues of patients with ESCC. Data were normalized by expression level of SNORD gene as an internal control. The GraphPad Prism and SPSS software were used for analysis of data. Results: MiR-196a expression was higher in cancer tissues compared to normal tissues (P>0.05). The miRNA upregulation had no significant correlation with clinicopathological features such as age, gender and survival rate. Conclusion: The results of the present study indicate a nonsignificant increased expression of miR-196a in ESCC tumor specimen. Future studies with a larger sample size could confirm our findings.

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“… 3 Growing evidence shows that some miRs have a tumor-promoting or -suppressing function, and miR expression is aberrant in many kinds of malignant tumors, including ESCC. 4 , 5 Additionally, some cancer-related miRs are detectable and stable in body fluids, indicating the potential of circulating miRs as a new class of biomarkers for the early diagnosis, prognosis prediction, and therapeutic evaluation of patients with cancer. 6 , 7 For example, plasma miR-92a-2 has been identified as a noninvasive diagnostic biomarker for small cell lung cancer.…”

Section: Introductionmentioning

confidence: 99%

Plasma microRNA-9 as a diagnostic and prognostic biomarker in patients with esophageal squamous cell carcinoma

et al. 2017

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PurposeEmerging evidence indicates that circulating microRNAs (miRs) might act as noninvasive biomarkers for cancer diagnosis and prognosis. We examined the expression pattern and clinical significance of plasma miR-9 in patients with esophageal squamous cell carcinoma (ESCC).MethodsVenous blood samples (6 mL) were collected from 131 patients with ESCC and 131 healthy controls, and the plasma miR-9 concentration was detected by reverse transcription polymerase chain reaction. The association of plasma miR-9 expression with clinicopathologic factors and survival of patients with ESCC was evaluated. Receiver operating characteristic (ROC) curve analysis was applied to evaluate the clinical value of plasma miR-9 for ESCC diagnosis.ResultsThe plasma miR-9 expression levels in patients with ESCC were significantly upregulated compared with normal controls. High plasma miR-9 concentrations were significantly correlated with poor tumor differentiation, large tumor size, deep local invasion, lymph node metastasis, advanced clinical stage, and poor survival. ROC curve analysis showed that the plasma miR-9 concentration could efficiently distinguish patients with ESCC from healthy controls. Multivariate survival analysis confirmed plasma miR-9 as an independent prognostic factor for ESCC.ConclusionsPlasma miR-9 expression was upregulated in ESCC and might act as a novel diagnostic and prognostic biomarker.

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MicroRNA-196a as a Potential Diagnostic Biomarker for Esophageal Squamous Cell Carcinoma

Cited by 25 publications

References 30 publications

miR‑196a regulates the proliferation, invasion and migration of esophageal squamous carcinoma cells by targeting ANXA1

miR‑196a regulates the proliferation, invasion and migration of esophageal squamous carcinoma cells by targeting ANXA1

Expression Analysis of MicroRNA-196a in Esophageal Cancer

Plasma microRNA-9 as a diagnostic and prognostic biomarker in patients with esophageal squamous cell carcinoma

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