MicroRNA-194 Inhibits the Epithelial–Mesenchymal Transition in Gastric Cancer Cells by Targeting FoxM1

Li, Zhenjun; Xiao, Ying; Chen, Hongliang; Ye, Pingjiang; Shen, Yi; Pan, Wei; Zhang, Lihua

doi:10.1007/s10620-014-3159-6

Cited by 48 publications

(44 citation statements)

References 20 publications

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“…Importantly, comparison between our current serum based study and a previous study undertaken in our laboratory which evaluated tissue based miRNAs associated with development of esophageal adenocarcinoma identified an overlap of 4 miRNAs, miR-194-5p, miR30a-5p, miR-15b-5p and miR-17-5p [20]. miR-194-5p and miR-30a-5p have been shown to be associated with epithelial-mesenchymal transition, which is one of the hallmarks of more aggressive cancers [21,22]. Recent studies have demonstrated an important role for miR-15b-5p (and miR-16-5p that lies in the same genomic cluster), in regulating apoptosis, cell cycle and DNA repair pathways [23], and highlighted miR-15b-5p as a potential target for anti-cancer therapies [24].…”

Section: Discussionsupporting

confidence: 57%

Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma

Chiam

Wang

Watson

et al. 2015

Journal of Gastrointestinal Surgery

121

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Section: Discussionsupporting

confidence: 57%

Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma

Chiam

Wang

Watson

et al. 2015

Journal of Gastrointestinal Surgery

121

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“…As for whether H19 [38,39]. More specifically, miR-194 could suppress invasion of gastric cancer and liver cancer cells, and miR-194's interfering with the EMT process of tumor cells also strengthened this effect [21,40]. Another investigation found that miR-194 might motivate formation of colonic neoplasms by targeting AKT2, and this effect was mediated by PI3K/Akt/mTOR signaling pathway [41].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the effect exerted by miR-194 on FoxM1 was suggested to be involved in the EMT process of neoplasms [21], and several microarray assays revealed that FoxM1 was highly expressed within > 20 solid neoplasms, including hepatocellular carcinoma, pancreatic adenocarcinoma, mastocarcinoma, ovarian cancer, lung cancer and CRC [43][44][45][46][47]. Besides, FoxM1 was over-expressed within actively-proliferating tumor cells, and it functioned to facilitate cell multiplication mainly by regulating cell cycle-specific genes [48].…”

Section: Discussionmentioning

confidence: 99%

The Effect of LncRNA H19/miR-194-5p Axis on the Epithelial-Mesenchymal Transition of Colorectal Adenocarcinoma

Chang-feng

Wang³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Since the combined actions of lncRNAs and miRNAs have been considered to be involved in the occurrence and development of various neoplasms, the main purpose of this study was to discover whether and how lncRNA H19 and miR-194 influenced the epithelial-mesenchymal transition (EMT) process of colorectal adenocarcinoma (CRA). Methods: Totally 214 pairs of CRA and adjacent normal tissues were collected, and 5 human CRA cell lines (i.e. HCT116, HT-29, RKO SW280 and Lovo) were purchased. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was adopted to quantify the H19 and miR-194-5p expressions in cells and tissues. The expressions of FoxM1, E-cadherin, vimentin, N-cadherin were determined using western blot. On the side, si-H19, si-NC, miR-194-5p mimic, miR-194-5p inhibitor and negative control (NC) were transfected into CRA cell lines. Meanwhile, the invasive, migratory and proliferative conditions of the cells were assessed through transwell, wound healing and colony-forming experiments, with final verification of the relationship between H19 and miR-194-5p employing dual-luciferase reporter gene assay. Results: Highly-expressed H19, lowly-expressed miR-194-5p, low-grade differentiation and lymph node metastasis appeared as the independent predictors of unfavorable prognosis in CRA patients’ (all P< 0.05). It indicated that FoxM1 expression displayed positive correlations with H19 expression, yet negative associations with miR-194-5p expression within CRA tissues (P< 0.05). In addition, transfection of H19-siRNA and miR-145-5p mimic triggered a conspicuous increase in E-cadherin expression, as well as an evidently down-regulation in vimentin and N-cadherin expressions within HT29 and RKO cells (P< 0.05). On the other hand, the invasive and migratory capacities of CRA cells were significantly hindered (P< 0.05). Moreover, the luciferase reporter gene assay confirmed that H19 modified miR-194-5p expression through directly targeting at it (P< 0.05). Ultimately, FoxM1 could reverse the role of miR-194-5p in inhibiting invasion, migration and EMT of CRA cells (P< 0.05). Conclusion: LncRNA H19/miR-194/FoxM1 axis could serve as a profound target for the diagnosis and treatment of CRA.

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“…For example, Han et al demonstrated that overexpression of miR-194 significantly repressed the proliferation, migration, and invasion of osteosarcoma cells in vitro, as well as tumour growth and pulmonary metastasis in vivo by targeting cadherin-2 (CDH2) and insulin-like growth factor 1 receptor (IGF-1R) [12]. In addition, it has been reported that miR-194 inhibited the EMT of EC cells [14] and gastric cancer cells [18]. MiR-194 has been identified as a prognostic marker of clear cell renal cell carcinoma (ccRCC) [19], myelodysplastic syndromes (MDS) [20], and HCC [15].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-194 Regulates Lipopolysaccharide-Induced Cell Viability by Inactivation of Nuclear Factor-κ B Pathway

Xie

Yang

Han

et al. 2017

Cell Physiol Biochem

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Abstract:The present study explored the functional role of microRNA (miR)-194 in lipopolysaccharide (LPS) induced lung cell injury, along with the underlying mechanisms and to reveal the potential role in infantile pneumonia. Human fibroblasts WI38 cells were transfected with miR-194 mimic or miR-194 inhibitor, and the transfection efficiency was confirmed by quantitative realtime polymerase chain reaction (qRT-PCR). Thereafter, the cells were treated with or without LPS, and then cell viability, cell apoptosis and mRNA and protein expressions of key proteins of nuclear factor kappa B (NF-κB) pathway including inhibitor of NF-κB (IκB) α, p-65, and B-cell CLL/lymphoma (Bcl) 3 were analyzed. Results showed that overexpression and suppression of miR-194 was effective. Administration of LPS significantly decreased the cell viability and statistically promoted the percentages of apoptotic cells and increased the mRNA and protein expressions of p-65 and Bcl-3 but downregulated IκBα compared to the control group (P < 0.05 or P < 0.01). LPS in combination with miR-194 suppression further enhanced the effects of LPS on cell viability and cell apoptosis compared to the LPS group (P < 0.05). In contrast, LPS in combination with miR-194 overexpression observably reversed the effects of LPS on cell viability, cell apoptosis and mRNA and protein expressions of the key proteins (P < 0.05 or P < 0.01). In conclusion, miR-194 increases the LPS-induced the inhibition of cell viability and increasing of the cell apoptosis by inhibition of NF-κB pathway in WI38 cells. MiR-194 might be a potential targeted therapy for treatment of infantile pneumonia.

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MicroRNA-194 Inhibits the Epithelial–Mesenchymal Transition in Gastric Cancer Cells by Targeting FoxM1

Abstract: Our study clearly demonstrates that miR-194 inhibits the acquisition of the EMT phenotype in gastric cancer cells by downregulating FoxM1, thereby inhibiting cell migration and invasion during cancer progression.

Cited by 48 publications

References 20 publications

Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma

Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma

The Effect of LncRNA H19/miR-194-5p Axis on the Epithelial-Mesenchymal Transition of Colorectal Adenocarcinoma

MicroRNA-194 Regulates Lipopolysaccharide-Induced Cell Viability by Inactivation of Nuclear Factor-κ B Pathway

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