MicroRNA 193b-3p as a predictive biomarker of chronic kidney disease in patients undergoing radical nephrectomy for renal cell carcinoma

Trevisani, Francesco; Ghidini, Michele; Larcher, Alessandro; Lampis, Andrea; Lote, Hazel; Manunta, Paolo; Alibrandi, Maria Teresa Sciarrone; Zagato, Laura; Citterio, Lorena; Dell’Antonio, Giacomo; Carenzi, Cristina; Capasso, Giovambattista; Rigotti, Paolo; Bertini, Roberto; Cascione, Luciano; Briganti, Alberto; Salonia, Andrea; Benigni, Fabio; Braconi, Chiara; Fassan, Matteo; Hahne, Jens C.; Montorsi, Francesco; Valeri, Nicola

doi:10.1038/bjc.2016.329

Cited by 32 publications

(23 citation statements)

References 39 publications

(40 reference statements)

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“…Our pathway enrichment results also showed the dysregulation of TGF-beta signaling pathway. Moreover, miR-193b-3p is predictive biomarkers of renal cell carcinoma [76]. The high expression of miR-615-3p was associated with pathogenesis of CRC and gastric cancer [77,78].…”

Section: Discussionmentioning

confidence: 96%

Identification of Prognostic Biomarker Signatures and Candidate Drugs in Colorectal Cancer: Insights from Systems Biology Analysis

Rahman¹,

Islam²,

Göv³

et al. 2018

Preprint

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Background and objectives: Colorectal cancer (CRC) is the second most common cause of cancer-related death in the world, but early diagnosis ameliorates the survival of CRC. This report directed to identify molecular biomarker signatures in CRC. Materials and Methods: We analyzed two microarray datasets (GSE35279 and GSE21815) from Gene Expression Omnibus (GEO) to identify mutual differentially expressed genes (DEGs). We integrated DEGs with protein-protein interaction and transcriptional/post-transcriptional regulatory networks to identify reporter signaling and regulatory molecules; utilized functional overrepresentation and pathway enrichment analyses to elucidate their roles in biological processes and molecular pathways; performed survival analyses to evaluate their prognostic performance; and applied drug repositioning analyses through Connectivity map (CMap) and geneXpharma tools to hypothesize possible drug candidates targeting reporter molecules. Results: A total of 727 up-regulated and 99 down-regulated DEGs were detected. The PI3K-Akt signaling, Wnt signaling, ECM-interaction, and cell cycle were identified as significantly enriched pathways. Ten hub proteins (ADNP, CCND1, CD44, CDK4, CEBPB, CENPA, CENPH, CENPN, MYC, and RFC2), 10 transcription factors (ETS1, ESR1, GATA1, GATA2, GATA3, AR, YBX1, FOXP3, E2F4, and PRDM14) and 2 miRNAs (miR-193b-3p and miR-615-3p) were detected as reporter molecules. The survival analyses through Kaplan Meier curves indicated remarkable performance of reporter molecules in estimation of survival probability in CRC patients. In addition, several drug candidates including anti-neoplastic and immunomodulating agents were repositioned. Conclusions: This study presents biomarker signatures at protein and RNA levels with prognostic capability in CRC. We think that the molecular signatures and candidate drugs presented in this study might be useful in future studies indenting development of accurate diagnostic and/or prognostic biomarker screens and efficient therapeutic strategies in CRC.

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Section: Discussionmentioning

confidence: 96%

Identification of Prognostic Biomarker Signatures and Candidate Drugs in Colorectal Cancer: Insights from Systems Biology Analysis

Rahman¹,

Islam²,

Göv³

et al. 2018

Preprint

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“…miR-320 could be a novel diagnosis and treatment target in renal ischemic reperfusion injury [ 19 ]. miR-193b-3p could also be a predictive biomarker in chronic kidney disease undergoing radical nephrectomy for renal cell carcinoma [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

miR-217 Is a Useful Diagnostic Biomarker and Regulates Human Podocyte Cells Apoptosis via Targeting TNFSF11 in Membranous Nephropathy

Liu

Xue³

et al. 2017

BioMed Research International

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Background MicroRNAs have recently been verified as useful diagnostic biomarkers in various diseases. In this study, we investigated whether miR-217 is a useful diagnostic biomarker and the possible pathological mechanism of miR-217 in this disease. Methods Patients with focal segmental glomerulosclerosis (FSGS), membranous nephropathy (MN), and diabetic nephropathy (DN) and control patients were enrolled in this study. The miR-217 inhibitor and mimics were transfected into human podocyte cells to investigate the pathological mechanism of miR-217 in this disease. Relevant indicators were detected and tested. Results Compared with control patients, miR-217 was significantly downregulated and TNFSF11 was significantly upregulated in MN. Then, miR-217 had obvious separation between patients with MN and control patients, with an AUC of 0.941, a cutoff value of <750.0 copies/ul, and sensitivity and specificity of 88.9% and 75.9%. In addition, the TNFSF11 was confirmed to be the target gene of miR-217. Finally, in in vitro experiments, the upregulation of miR-217 could decrease the expression of TNFSF11 and not induce human podocyte cells apoptosis; however, the downregulation of miR-217 could bring about an opposite change. Conclusions miR-217 is a useful diagnostic biomarker and is involved in human podocyte cells apoptosis via targeting TNFSF11 in membranous nephropathy.

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“…Renal cell carcinoma (RCC) is the 9th most common cancer diagnosis and cause of cancer-associated mortality, and accounts for 3% of all malignancies worldwide (1,2). Considering that, at present, there are no available biomarkers for RCC screening, ~30% of patients develop metastasis at the time of diagnosis, and 20-40% patients develop recurrence or metastases following initial surgical resection (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

microRNA‑181a‑5p functions as an oncogene in renal cell carcinoma

Lai

Zhao

et al. 2018

Mol Med Report

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Renal cell carcinoma (RCC) is one of the most common urinary tumors. Previous studies have demonstrated that microRNA (miR)‑181a‑5p has an important role in numerous types of cancer. However, the function of miR‑181a‑5p in RCC remains unknown. In the present study, the expression levels of miR‑181a‑5p in RCC tissues and cell lines were investigated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. The results of the RT‑qPCR analyses suggested that the expression of miR‑181a‑5p was upregulated in RCC tissues and cells lines compared with adjacent normal renal tissues and normal renal cell lines. Furthermore, the effect of miR‑181a‑5p on cell proliferation, migration, invasion and apoptosis was investigated in the present study. Overexpression of miR‑181a‑5p was revealed to suppress the apoptosis of 786‑O and ACHN cells, in addition to enhancing the proliferation, migration and invasion abilities of 786‑O and ACHN cells in vitro, thus suggesting that miR‑181a‑5p may function as an oncogene in RCC. However, further studies are required to investigate the underlying mechanism of miR‑181a‑5p and its potential role as a biomarker for early detection and prognosis, in addition to as a therapeutic target in RCC.

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MicroRNA 193b-3p as a predictive biomarker of chronic kidney disease in patients undergoing radical nephrectomy for renal cell carcinoma

Cited by 32 publications

References 39 publications

Identification of Prognostic Biomarker Signatures and Candidate Drugs in Colorectal Cancer: Insights from Systems Biology Analysis

Identification of Prognostic Biomarker Signatures and Candidate Drugs in Colorectal Cancer: Insights from Systems Biology Analysis

miR-217 Is a Useful Diagnostic Biomarker and Regulates Human Podocyte Cells Apoptosis via Targeting TNFSF11 in Membranous Nephropathy

microRNA‑181a‑5p functions as an oncogene in renal cell carcinoma

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