MicroRNA-192 promotes the development of nasopharyngeal carcinoma through targeting RB1 and activating PI3K/AKT pathway

Huang, Qingli; Hou, Sen; Zhu, Xuemei; Liu, Shouzhou

doi:10.1186/s12957-020-1798-y

Cited by 16 publications

(19 citation statements)

References 35 publications

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“…3F). Among these, the PI3K-Akt pathway has been previously implicated in NPC progression [24,25]. Our ndings are in line with those of previous studies.…”

Section: Go and Kegg Of Predicted Target Genessupporting

confidence: 93%

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Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing 

Zheng

Huang

et al. 2020

Preprint

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Background. Early diagnosis of nasopharyngeal carcinoma (NPC) is vital to improve the prognosis of these patients. However, early diagnosis of NPC is typically challenging. Therefore, we explored the roles and mechanisms of exosomes in plasma of patients with early-stage NPC. Methods. Exosomes in plasma were extracted by ultra-high-speed centrifugation. Western blot and transmission electron microscopy (TEM) were used to verify the purity of exosomes. The sequencing data (6 plasma samples from healthy volunteers vs. 6 NPC plasma samples) were analyzed by principal component analysis (PCA), DESeq2, gene ontology (GO), kyoto encyclopedia of genes and genomes (KEGG), and TargetScan. The differentially expressed miRNAs (DEmiRNAs) were obtained from the dataset (GSE118720) downloaded from the Gene Expression Omnibus (GEO) repository. Additionally, the datasets downloaded from the GEO database (GSE12452, GSE13597, GSE53819, GSE64634) were used to predict the target genes and functions of hsa-miR-1301-3p. qPCR was applied to verify the differences in the expressions of hsa-miR-1301-3p between 10 normal plasma and 10 NPC plasma samples.Results. Western blot, TEM, and Nanoparticle Tracking Analysis showed adequate purity of the extracted exosomes. RNA-seq analysis revealed 21 upregulated miRNAs, and 10 downregulated miRNAs in plasma exosomes of early-stage NPC patients. GO analysis showed that the target genes of DEmiRNAs were mainly enriched in DNA synthesis and transcription regulation. KEGG analysis revealed that DEmiRNAs were mainly enriched in PI3K-Akt and MAPK signaling pathways. Moreover, the expression of hsa-mir-1301-3p was verified to be significantly upregulated in enlarged samples of plasma exosomes.Conclusions. We identified several DEmiRNAs extracted from tumor-derived exosomes between normal plasma and early-stage NPC plasma. Bioinformatics analyses indicated that these DEmiRNAs may be related to NPC development. Our study may provide novel insights into underlying biomarkers and mechanisms of plasma exosomes in early-stage NPC.

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“…3F). Among these, the PI3K-Akt pathway has been previously implicated in NPC progression [24,25]. Our ndings are in line with those of previous studies.…”

Section: Go and Kegg Of Predicted Target Genessupporting

confidence: 93%

“…Early diagnosis of NPC is a key strategy for improving the prognosis of patients [25,26]. However, accurate early diagnosis of NPC is typically challenging [27].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing 

Zheng

Huang

et al. 2020

Preprint

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“…Moreover, tissue-derived and serum-exosomal expression of miR-192-5p could discriminate between PDAC and HCs with good accuracy, similar to that of established tumor marker CA.19-9. However, the clinical value of miR-192-5p remains ambiguous, as previous studies investigating its regulation in PDAC revealed in part contradictory results ( Table S1 [ 27 , 28 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ]). For the first time in 2013, Zhao et al described an overexpression of miR-192-5p in tumor tissue and blood serum of PDAC patients [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, decreased levels of circulating miR-192-5p were shown to correlate with presence of bone metastasis in non-small cell lung cancer (NSCLC) [ 37 ]. Contrarily, its upregulation in tissue of esophageal squamous cell carcinoma, prostate cancer, and nasopharyngeal carcinoma illustrated the different tumor specific signatures of miR regulation [ 38 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Tumor-Suppressive miR-192-5p Has Prognostic Value in Pancreatic Ductal Adenocarcinoma

Flammang

Reese

Yang

et al. 2020

Cancers

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Pancreatic ductal adenocarcinoma (PDAC) is characterized by fast tumor progression and diagnosis at advanced, inoperable stages. Previous studies could demonstrate an involvement of miR-192-5p in epigenetic regulation of visceral carcinomas. Due to contradictory results, however, the clinical utility of miR-192-5p in PDAC has yet to be determined. MiR-192-5p expression was analyzed by RT-qRT-PCR in human PDAC and benign tissue (n = 78), blood serum (n = 81) and serum exosomes (n = 74), as well as in PDAC cell lines (n = 5), chemoresistant cell clones (n = 2), and pancreatic duct cell line H6c7. Analysis of EMT-associated (epithelial-to-mesenchymal transition) proteins was performed by immunohistochemistry and Western blot. MiR-192-5p was deregulated in PDAC as compared to healthy controls (HCs), with downregulation in macrodissected tissue (p < 0.001) and upregulation in blood serum of PDAC UICC (Union for International Cancer Control) stage IV (p = 0.016) and serum exosomes of PDAC UICC stages II to IV (p < 0.001). MiR-192-5p expression in tumor tissue was significantly lower as compared to corresponding peritumoral tissue (PDAC UICC stage II: p < 0.001; PDAC UICC stage III: p = 0.024), while EMT markers ZEB1 and ZEB2 were more frequently expressed in tumor tissue as compared to peritumoral tissue, HCs, and chronic pancreatitis. Tissue-derived (AUC of 0.86; p < 0.0001) and exosomal (AUC of 0.83; p = 0.0004) miR-192-5p could differentiate between PDAC and HCs with good accuracy. Furthermore, high expression of miR-192-5p in PDAC tissue of curatively resected PDAC patients correlated with prolonged overall and recurrence-free survival in multivariate analysis. In vitro, miR-192-5p was downregulated in gemcitabine-resistant cell clones of AsPC-1 (p = 0.029). Transient transfection of MIA PaCa-2 cells with miR-192-5p mimic resulted in downregulation of ZEB2. MiR-192-5p seems to possess a tumor-suppressive role and high potential as a diagnostic and prognostic marker in PDAC.

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“…Currently, radiotherapy and chemotherapy remain the standard treatments for late-stage NPC. 1 , 2 Despite the improvement of radiotherapy and chemotherapy in combating NPC, recurrence and metastasis are frequently observed in patients. 3 Hence, promising biomarkers and curative options are urgently warranted to improve the clinical prognosis in patients with NPC.…”

Section: Introductionmentioning

confidence: 99%

TMPO-AS1 Regulates the Aggressiveness-Associated Traits of Nasopharyngeal Carcinoma Cells Through Sponging miR-320a

Xing¹,

Qiao²,

Qiu³

et al. 2021

CMAR

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MicroRNA-192 promotes the development of nasopharyngeal carcinoma through targeting RB1 and activating PI3K/AKT pathway

Cited by 16 publications

References 35 publications

Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing

Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing

Tumor-Suppressive miR-192-5p Has Prognostic Value in Pancreatic Ductal Adenocarcinoma

TMPO-AS1 Regulates the Aggressiveness-Associated Traits of Nasopharyngeal Carcinoma Cells Through Sponging miR-320a

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MicroRNA-192 promotes the development of nasopharyngeal carcinoma through targeting RB1 and activating PI3K/AKT pathway

Cited by 16 publications

References 35 publications

Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing&nbsp;

Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing&nbsp;

Tumor-Suppressive miR-192-5p Has Prognostic Value in Pancreatic Ductal Adenocarcinoma

TMPO-AS1 Regulates the Aggressiveness-Associated Traits of Nasopharyngeal Carcinoma Cells Through Sponging miR-320a

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Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing

Identification of Potential Plasma Biomarkers in Early-stage Nasopharyngeal Carcinoma-derived Exosomes Based on RNA Sequencing