MicroRNA-192 inhibits the proliferation, migration and invasion of osteosarcoma cells and promotes apoptosis by targeting matrix metalloproteinase-11

Shang, Guowei; Mi, Yang; Mei, Yingwu; Wang, Guanghui; Wang, Yadong; Li, Xinjie; Wang, Yisheng; Li, Yuebai

doi:10.3892/ol.2018.8239

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…Growing evidence has revealed that miRNAs are involved in human diseases such as cancer, diabetes, hypertension, and cardiovascular disease [24]. Since a single miRNA can target several mRNAs, and being components of a larger regulatory network [25], miRNAs can affect cellular function, differentiation, proliferation, and apoptosis, as well as tumor formation, metastasis and drug resistance in carcinogenesis [26,27]. Here, we established and validated a 3-miRNA-based classifier to increase the accuracy of predicting the prognosis of patients with OS.…”

Section: Discussionmentioning

confidence: 99%

Identification and Validation of a Potent Multi-miRNA Signature for Prediction of Prognosis of Osteosarcoma Patients

et al. 2020

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Departmental sources Background: Osteosarcoma, the most common solid malignancy, has high incidence and mortality rates. We constructed a miRNA-based signature that can be used to assess the prognosis of osteosarcoma patients. Material/Methods: The miRNA profile was derived from the Gene Expression Omnibus (GEO) website, with matched clinical records. The miRNA-based overall survival (OS)-predicting signature was established by LASSO Cox regression analysis. Receiver operating characteristic (ROC) curve and Kaplan-Meier (K-M) analyses were performed to examine the stability and discriminatory ability of the OS-predicting signatures. Pathway enrichment analyses were performed to uncover potential mechanisms. Results: Three miRNAs (miR-153, miR-212, and miR-591) independently related to the OS were extracted to build a risk score formula. The ROC curve and K-M analyses revealed good discrimination ability of the OS signature for osteosarcoma patients in both the training cohort (P=0.00015, AUC=0.962) and the validation cohort (P=0.0065, AUC=0.793). As shown in multivariate analysis, the classifier showed favorable predictive accuracy similar to the recurrence status to be an independent risk factor for osteosarcoma. Furthermore, the nomogram showed a synergistic effect by combining the clinicopathological features with our classifier. Also, the enrichment analyses of the target genes may contribute to improved treatment of osteosarcoma. Conclusions: The 3-miRNA-based classifier serves as an effective prognosis-predicting signature for osteosarcoma patients.

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Section: Discussionmentioning

confidence: 99%

Identification and Validation of a Potent Multi-miRNA Signature for Prediction of Prognosis of Osteosarcoma Patients

et al. 2020

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“…Likewise, the results of another study demonstrated that both the expression of miRNA-192-3p and miRNA-192-5p were decreased in stage IIIB colon cancer as compared to healthy tissues (32). The reduction in the expression level of this tumor suppressor miRNA has been reported in osteosarcoma, lung cancer and pancreatic cancer, where it has been reported that miR-192 exhibits a tight association with the regulation of cell proliferation and apoptosis (33)(34). Moreover, it has been reported that the downregulation of miR-192 in hepatocellular carcinoma (HCC) may provide a signal that upregulates SLC39A6/SNAIL, a molecule involved in cell invasion, which in turn deteriorates patient outcome (35).…”

Section: Discussionmentioning

confidence: 71%

Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer

et al. 2019

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Given the global outbreak of breast cancer and its debilitating effect on women's health, it is not surprising that tremendous efforts have been made with an aim of shedding more light on the mechanisms involved in the pathogenesis of this type of cancer. Among the long list of risk factors associated with this malignancy, recently, the role of microRNAs (miRNAs or miRs) has turned into a hotspot for breast cancer investigations. miRNAs approximately 20 nucleotides in length and are located in either an exon or an intron, playing a role in the regulation of gene expression. In the present study, we extracted RNA from both the serum and cancerous tissue of breast cancer patients and after synthesizing the cDNA, we performed quantitative PCR to determine the expression levels of miR-9 and miR-192. The resulting data revealed that while the mRNA expression level of miR-9 was significantly decreased in the breast cancer tissues, there was no noticeable change in the expression level of this miRNA in the serum samples. Likewise, we found that the marked downregulation of miR-192 was only restricted to the cancerous tissues, but was not found in the serum of patients. Based on the meaningful downregulation of the expression of miR-9 and miR-192, this study provides a plausible framework for these miRNAs as effective biomarkers for breast cancer patients.

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“…Besides, a series of studies reported that miRNA‐192 and miRNA‐215 have been identified as a tumor suppressor in osteosarcoma (Shang et al, 2018), colon cancer (Vychytilova‐Faltejskova et al, 2017), epithelial ovarian (Lin, Jin, Xu, Zhou, & Cui, 2017), and whereas it may act as an oncogene in hepatocellular carcinoma (Yan‐Chun et al, 2017), endometrial cancer (Gao, Cai, & An, 2018), and GC (Jin et al, 2011; Xu & Fan, 2015; X. Zhang et al, 2018a, 2018b). miRNA‐192 may function as a tumor suppressor in osteosarcoma cells (OS), and capable of inhibiting the proliferation, migration, and invasion of OS and promotes apoptosis by targeting matrix metalloproteinase‐11 (Shang et al, 2018). However, a study demonstrates that miRNA‐192‐5p can promote the proliferation and metastasis of hepatocellular carcinoma cell by targeting SEMA3A (Yan‐Chun et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

miRNA‐192 and ‐215 activate Wnt/β‐catenin signaling pathway in gastric cancer via APC

Deng

Zhang

Qin

et al. 2020

Journal Cellular Physiology

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Although great progress has been made in surgical techniques, traditional radiotherapy, and chemotherapy, gastric cancer (GC) is still the most common malignant tumor and has a high mortality, which highlights the importance of novel diagnostic markers. Emerging studies suggest that different microRNAs (miRNAs) are involved in tumorigenesis of GC. In this study, we found that miRNA-192 and -215 are significantly upregulated in GC and promote cell proliferation and migration. Adenomatous polyposis coli (APC), a well-known negative regulator in Wnt signaling, has been proved to be a target of miRNA-192 and -215. Inhibition of miRNA-192 or -215 reduced the Topflash activities and repressed the expression of Wnt signaling pathway proteins, while APC small interfering RNAs reversed the inhibitory effects, suggesting that miRNA-192 and -215 activate Wnt signaling via APC. In addition, APC mediates the cell proliferation and migration regulated by miRNA-192 and -215.Furthermore, APC is downregulated in GC tissues and negatively correlated with the expression of target APC and function as oncogenic miRNAs by activating Wnt signaling in GC, revealing to be potential therapeutic targets.Shiqi Deng and Xiaojing Zhang contributed equally to this study.

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MicroRNA-192 inhibits the proliferation, migration and invasion of osteosarcoma cells and promotes apoptosis by targeting matrix metalloproteinase-11

Cited by 16 publications

References 36 publications

Identification and Validation of a Potent Multi-miRNA Signature for Prediction of Prognosis of Osteosarcoma Patients

Identification and Validation of a Potent Multi-miRNA Signature for Prediction of Prognosis of Osteosarcoma Patients

Evaluation of microRNA‑9 and ‑192 expression levels as biomarkers in patients suffering from breast cancer

miRNA‐192 and ‐215 activate Wnt/β‐catenin signaling pathway in gastric cancer via APC

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