MicroRNA-183-5p acts as a potential diagnostic biomarker for atherosclerosis and regulates the growth of vascular smooth muscle cell

Abstract

Background Atherosclerosis (AS) is a multifactorial chronic disease, and vascular smooth muscle cells (VSMCs) plays an important role in the pathology of AS. MicroRNAs regulate multiple cellular biological processes. This study aimed to investigate the clinical value of miR-183-5p in AS patients, and further explored the effects of miR-183-5p on the proliferation and migration of VSMCs. Methods qRT-PCR was used to test the level of miR-183-5p. The diagnostic value of miR-183-5p for AS patients was assessed by a receiver operating characteristic (ROC) analysis. Cell proliferation and migration were determined via CCK-8 and Transwell assay. Results MiR-183-5p was highly expressed in AS patients compared with the healthy group. Serum miR-183-5p expression was positively associated with CIMT and CRP in AS patients. The ROC analysis suggested that miR-183-5p had quality to be used as a biomarker with high specificity and sensitivity for AS detection. Overexpression of miR-183-5p promoted the proliferation and migration of VSMCs. Downregulation of miR-183-5p attenuated ox-LDL stimulated VSMCs proliferation and migration. Conclusion MiR-183-5p is highly expressed in AS patients, and downregulation of miR-183-5p attenuated ox-LDL stimulated VSMCs proliferation and migration. MiR-183-5p may be a key molecular for the diagnosis and treatment of AS in the future.

In silico Prediction of miRNA Interactions With Candidate Atherosclerosis Gene mRNAs

“…In most cases, polygenic diseases, including atherosclerosis, develop when the expression of different combinations of candidate genes changes. As a result of a review of various publications, it seems that the approaches used to search for markers for the diagnosis and treatment of atherosclerosis, including miRNA, have not yet solved the problem of diagnosing and treating atherosclerosis ( Chen et al, 2020 ; Li et al, 2020 ; Ryu et al, 2020 ; Sun et al, 2020 ; Wang W. et al, 2020 ; You et al, 2020 ). In human, it is known about 7000 miRNA and more than 20,000 genes, and it is unknown how many miRNA and genes from them participate in the development of atherosclerosis ( Byrne et al, 2014 ; Toba et al, 2014 ; Lu et al, 2018 ; Liu et al, 2020 ; Shi et al, 2020 ).…”