MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2

Chen, Gong; Zhu, Wenjun; Shi, Dezhi; Li, LV; Zhang, Chun; Liu, Ping; Hu, Weixing

doi:10.3892/or_00000725

Cited by 84 publications

(21 citation statements)

References 20 publications

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“…Consistent with our hypothesis, miR-181a sensitized K562/A02 and SGC7901/VCR cell lines by targeting Bcl-2 [15,16]. Curretly 28 target genes of hsa-miR-181a have been validated [10,11,12,17,18]. The presence of many matching sites between the mature miR-181a seed region have facilitated the role of miR-181a in chemotherapy, giving them access to the modulation of a huge number of cellular pathways, especially apoptosis or anti-apoptosis ones.…”

Section: Introductionsupporting

confidence: 60%

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The Function Role of miR-181a in Chemosensitivity to Adriamycin by Targeting Bcl-2 in Low-Invasive Breast Cancer Cells

Zhu

Wu²,

Li³

et al. 2013

Cell Physiol Biochem

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Objectives: miR-181a is involved in immunity, metabolism, tumor suppression or carcinogenesis reported by many other studies. However, its role in the development of chemosensitivity to adriamycin in low-invasive breast cancer cells remains unclear. The aim of this study is to define the function role of miR-181a in promoting the efficacy of adriamycin-based neoadjuvant chemotherapy. Methods: Cell survival analysis was detected by Cell Counting Kit-8 assay. Apoptotic cells were quantitatively detected using FITC Annexin V apoptosis Detection Kit I. Bcl-2 protein expression was measured by western blot. Luciferase reporter vector with the putative BCL-2 3' untranslated region (3'UTR) was constructed to explore whether BCL-2 was a direct target gene of miR-181a. Real-time PCR was performed to test the expression of miR-181a and Bcl-2 in the selected breast cancer tissue samples. Results: The down-regulation of miR-181a decreased adriamycin-induced apoptosis in MCF-7 cells. Transfected with miR-181a mimic in cells resulted in the decreased expression of Bcl-2. The alteration of miR-181a expression did not significantly affect the chemosensitivity to adriamycin in MCF-7 and MCF-7/ADR cells with genetic knockout of Bcl-2. miR-181a may suppress Bcl-2 expression by forming imperfect base pairing with the 3'UTR of Bcl-2 gene such that a negative relationship between miR-181a and Bcl-2 in MCF-7 and MCF-7/ADR cells is observed. Conclusions: At least in part, the detection of miR-181a may direct the clinical medication in patients with neoadjuvant chemotherapy because of miR-181a enhanced adriamycin-induced apoptosis via targeting Bcl-2.

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Section: Introductionsupporting

confidence: 60%

“…It is still controversial that miR-181a acts as a oncomir or a tumor suppressor. The global downregulation of miR-181a was observed in most soild tumors [17,18,23,24,25,26] and chronic lymphocytic leukaemia (CLL) [27]. On the contrary, the role of miR-181a in breast cancer was confirmed recently.…”

Section: Discussionmentioning

confidence: 96%

The Function Role of miR-181a in Chemosensitivity to Adriamycin by Targeting Bcl-2 in Low-Invasive Breast Cancer Cells

Zhu

Wu²,

Li³

et al. 2013

Cell Physiol Biochem

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“…Recent studies have reported the involvement of miR-181a in diverse cellular functions such as growth, proliferation, death, survival, and maintenance, as well as tumor suppression and carcinogenesis [10, 11]. Though some studies have shown that miR-181a expression is downregulated in several human solid tumors [12-17], others have demonstrated that upregulation of miR-181a may promote metastasis and invasion of human cancers [18-20]. …”

Section: Introductionmentioning

confidence: 99%

The Dual Regulatory Role of MiR-181a in Breast Cancer

Yang

Tabatabaei

Ruan

et al. 2017

Cell Physiol Biochem

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MicroRNAs (miRNAs) are a family of highly conserved noncoding single˗stranded RNA molecules of 21 to 25 nucleotides. miRNAs silence their cognate target genes at the post-transcriptional level and have been shown to have important roles in oncogenesis, invasion, and metastasis via epigenetic post-transcriptional gene regulation. Recent evidence indicates that the expression of miR-181a is altered in breast tumor tissue and in the serum of patients with breast cancer. However, there are several contradicting findings that challenge the biological significance of miR-181a in tumor development and metastasis. In fact, some studies have implicated miR-181a in regulating breast cancer gene expression. Here we summarize the current literature demonstrating established links between miR-181a and human breast cancer with a focus on recently identified mechanisms of action. This review also aims to explore the potential of miR-181a as a diagnostic and/or prognostic biomarker for breast cancer and to discuss the contradicting data regarding its targeting therapeutics and the associated challenges.

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“…The over-expression of Notch2 stimulates the malignancy of gliomas and GSC while the introduction of mir-181a successfully inhibits them (57). Another study confirmed miR-181a as a potential glioblastoma treatment by sensitising the glioblastomas to radiation through B-cell lymphoma 2 protein (Bcl-2) downregulation, an anti-apoptotic protein (59, 60).…”

Section: Normal Functions Of Ucmsc’s Extracellular Vesicles and Micromentioning

confidence: 90%

Advancing towards Effective Glioma Therapy: MicroRNA Derived from Umbilical Cord Mesenchymal Stem Cells’ Extracellular Vesicles

Ngadiono

Hardiany

2019

MJMS

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A glioma, especially a grade IV glioblastoma, is a malignant tumour with a poor prognosis despite growing medical advancements. Researchers have been looking for better and more effective treatments targeting the molecular pathways of gliomas due to glioblastomas’ ability to develop resistance to chemotherapies. Moreover, glioma stem cells (GSC) contribute to maintaining the glioma population, which benefits from its ability to self-renew and differentiate. Recent research has reported that through the introduction of umbilical cord mesenchymal stem cells (UCMSC) into glioma cells, the growth and development of the glioma cells can be downregulated. It has more currently been found out that UCMSC release extracellular vesicles (EVs) containing miRNA that are responsible for this phenomenon. Therefore, this review analyses literature to discuss all possible miRNAs contained within the UCMSC’s EVs and to elaborate on their molecular mechanisms in halting gliomas and GSC growth. This review will also include the challenges and limitations, to account for which more in vivo research is suggested. In conclusion, this review highlights how miRNAs contained within UCMSC’s EVs are able to downregulate multiple prominent pathways in the survival of gliomas.

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MicroRNA-181a sensitizes human malignant glioma U87MG cells to radiation by targeting Bcl-2

Cited by 84 publications

References 20 publications

The Function Role of miR-181a in Chemosensitivity to Adriamycin by Targeting Bcl-2 in Low-Invasive Breast Cancer Cells

The Function Role of miR-181a in Chemosensitivity to Adriamycin by Targeting Bcl-2 in Low-Invasive Breast Cancer Cells

The Dual Regulatory Role of MiR-181a in Breast Cancer

Advancing towards Effective Glioma Therapy: MicroRNA Derived from Umbilical Cord Mesenchymal Stem Cells’ Extracellular Vesicles

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