MicroRNA-181a-5p suppresses cell proliferation by targeting Egr1 and inhibiting Egr1/TGF-β/Smad pathway in hepatocellular carcinoma

Bi, Joffe; JinFeng, Zheng; Li, Qi; Bao, Shaowen; Yu, Xiaofang; Xu, Ping; Liao, Cai-xian

doi:10.1016/j.biocel.2018.11.011

Cited by 40 publications

(37 citation statements)

References 43 publications

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“…To explore the potential mechanism of BZW1, TGF-β1/Smad pathway was investigated. It is well known that TGFβ/Smad pathway is involved in cell proliferation [19][20][21]. Results shown that BZW1 over-expression activated the TGF-β1/Smad pathway.…”

Section: Discussionmentioning

confidence: 91%

BZW1 promotes cell proliferation in prostate cancer by regulating TGF-β1/Smad pathway

et al. 2021

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Background Recently, basic leucine zipper and the W2 domain-containing protein 1 (BZW1) is reported to be implicated in tumor progression. However, the role of BZW1 in prostate cancer remains unknown. This study is aimed to investigate the expression of BZW1 and its in uence on cell proliferation in prostate cancer.Methods The expression levels of BZW1 were measured in 136 cases of prostate cancer and matched adjacent non-cancerous prostate tissues by quanti cational real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). Then, the effect of BZW1 on cell proliferation was further explored.Results QRT-PCR analysis shown that the mRNA levels of BZW1 in prostate cancer were signi cantly greater compared with those in matched adjacent non-cancerous prostate tissues (P<0.001). IHC results shown the high-expression rate of BZW1 in prostate cancer and matched adjacent non-cancerous prostate tissues were 68.4% and 32.4%, and the difference was statistically signi cant (P<0.001). BZW1 high-expression signi cantly correlated with T stage, lymph node metastasis, prostate speci c antigen (PSA) and Gleason score (P<0.05). Patients with BZW1 high-expression presented unfavorable prognosis compared with those with BZW1 low-expression (P=0.002). In addition, CCK-8 and Colony formation assays revealed that BZW1 over-expression signi cantly promoted cell proliferation in vitro. Tumor xenograft shown BZW1 knockdown signi cantly inhibited tumor growth in vivo. Moreover, BZW1 overexpression activated the TGF-β1/Smad1/Smad3 pathway. Conclusion BZW1 over-expression predicts poorer prognosis and promotes cell proliferation in prostate cancer by regulating TGF-β1/Smad pathway.

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Section: Discussionmentioning

confidence: 91%

BZW1 promotes cell proliferation in prostate cancer by regulating TGF-β1/Smad pathway

et al. 2021

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“…a number of transcription factors, including activator protein 1, nF-κB and egr1, can regulate the expression of tumor necrosis factor-α (TnF-α), transforming growth factor β1 and other cytokines by various stimulating factors in different types of cells, such as macrophages and epidermal keratinocytes (46,47). in addition, a number of studies have observed that the transcription factor egr1 serves an important role in regulating the expression of these stimulating factors and affects the occurrence and development of diseases (48)(49)(50)(51)(52). These results suggested that egr1 may cause PaiTa via the regulation of PaiTa-associated pathogenic factors.…”

Section: Discussionmentioning

confidence: 97%

Effects of Egr1 on pancreatic acinar intracellular trypsinogen activation and the associated ceRNA network

et al. 2020

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acute pancreatitis (aP) is a common digestive disorder with high morbidity and mortality. The present study aimed to investigate the expression of early growth response protein 1 (egr1), and the effect of competing endogenous (ce)rna network on trypsinogen activation. Pancreatic acinar intracellular trypsinogen activation (PaiTa) is an important event in the early stage of aP; however, the underlying mechanisms remain unclear. The present study used taurolithocholic acid 3-sulfate (Tlc-S)-treated ar42J cells (pancreatic cell line) to establish a PaiTa model. a gene microarray and bioinformatics analysis was performed to identify the potential key targets in PaiTa. The results demonstrated that egr1, an important transcription factor, was significantly overexpressed in PAITA. In Egr1 small interfering (si)rna-transfected cells, egr1 expression was decreased and trypsinogen activation was significantly decreased compared with negative control sirna-transfected cells, indicating that in Tlc-S-induced PaiTa, overexpression of egr1 enhanced trypsinogen activation. a cerna network [mrna-microrna (mirna/mir)-long non-coding (lnc)rna] generated using the PaiTa model revealed that the effects of egr1 on PaiTa may be regulated by multiple cerna pairs, and the lncrnas (including nonraTT022624 and nonraTT031002) and mirnas [including Rattus norvegicus (rno)-mir-214-3p and rno-mir-764-5p] included in the cerna pairs may serve roles in PaiTa by regulating the expression of egr1. The results of the present study may provide novel targets for researching the underlying mechanisms of, and developing treatments for aP.

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“…The results indicate that elevated interpretation of A-Raf and FA2H in hepatocellular carcinoma is associated with lipid metabolism disorders and cancer progression [ 48 ]. Bi et al believe that miR-181a-5p is a negative regulator of Egr1 and can inhibit tumor proliferation in HCC by targeting the Egr1/TGF- β 1/Smad pathway, which may be a potential therapeutic approach for HCC [ 49 ]. The ubiquitin-binding enzyme E2S (UBE2S) has important functions in the development of human cancer.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Characteristics of Liver Tissue Reveal the Underlying Pathogenesis of Hepatocellular Carcinoma

Guo

Rong

et al. 2021

BioMed Research International

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Background. Hepatocellular carcinoma (HCC) is high-mortality primary liver cancer and the most common malignant tumor in the world. This study is based on a hepatocellular carcinoma-related dysfunction module designed to explore the dysregulation of genes in liver cancer tissue. Methods. By downloading the relevant data on the GEO database, we performed a differential analysis of healthy liver tissue and liver cancer tissues as well as healthy liver tissue and hepatocellular carcinoma tissue and then obtained two sets of differential genes and combined them. We performed a cointerpretation analysis of these differential genes and constructed related functional disorder modules. A hypergeometric test was performed to calculate the potential regulatory effects of multiple factors on the module, and a series of ncRNA and TF regulators were identified. We obtained a total of 4479 differentially expressed genes in hepatocellular carcinoma, and these genes were clustered into ten hepatocellular carcinoma-related functional interpretation disorder modules. Results. Enrichment analysis revealed that these modular genes are mainly involved in signal transduction including cell cycle, TGF-beta signal transduction, and p53 signal transduction. Depending on the predictive analysis of multidimensional regulators, 323 ncRNAs and 52 TF-mediated hepatocellular carcinoma-related dysregulation modules were found to regulate disease progression. Conclusions. Based on a series of investigations, it was found that miR-30b-5p may participate in the peroxisome signal transduction by downregulating ABCD3-mediated module 1, thereby promoting the development and progression of hepatocellular carcinoma. Our research results not only provide a theoretical basis for biologists to study hepatocellular carcinoma further but also offer new methods and new ideas for the personalized care and treatment of hepatocellular carcinoma.

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MicroRNA-181a-5p suppresses cell proliferation by targeting Egr1 and inhibiting Egr1/TGF-β/Smad pathway in hepatocellular carcinoma

Cited by 40 publications

References 43 publications

BZW1 promotes cell proliferation in prostate cancer by regulating TGF-β1/Smad pathway

BZW1 promotes cell proliferation in prostate cancer by regulating TGF-β1/Smad pathway

Effects of Egr1 on pancreatic acinar intracellular trypsinogen activation and the associated ceRNA network

Gene Expression Characteristics of Liver Tissue Reveal the Underlying Pathogenesis of Hepatocellular Carcinoma

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