MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis

Qiu, Hua; Liu, N; Luo, Lan; Zhong, Jiasheng; Tang, Zilong; Kang, Koo Jeong; Qu, Junle; Peng, Weiwen; Liu, L; Li, L; Gou, Deming

doi:10.1038/cdd.2016.56

Cited by 47 publications

(44 citation statements)

References 57 publications

(76 reference statements)

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“…Examples include ATBF1-A (ZFHX3) [49], Id1 [50, 51], Teashirt-3 [52], Hey-1 [53], HMGA1 [54], the MBD2-interacting zinc finger protein MIZF [55], Staufen1 [56], JAZF1 [57], Foxk1 [58], Bhlhe41 (DEC2, Sharp1) [44] or ZBTB38 (CIBZ) [37, 43]. Little is known about any association of these proteins to cullin-linked protein degradation.…”

Section: Discussionmentioning

confidence: 99%

Cullin E3 Ligase Activity Is Required for Myoblast Differentiation

Blondelle

Shapiro

Domenighetti

et al. 2017

Journal of Molecular Biology

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The role of cullin E3-ubiquitin ligases for muscle homeostasis is best known during muscle atrophy, as the cullin-1 substrate adaptor atrogin-1 counts among the most well-characterized muscle atrogins. We investigated, whether cullin activity was also crucial during terminal myoblast differentiation and aggregation of acetylcholine receptors for the establishment of neuromuscular junctions in vitro. The activity of cullin E3-ligases is modulated through posttranslational modification with the small ubiquitin-like modifier nedd8. Using either the Nae1 inhibitor MLN4924 (Pevonedistat) or siRNA against nedd8 in early or late stages of differentiation on C2C12 myoblasts, and primary satellite cells from mouse and human, we show that cullin E3-ligase activity is necessary for each step of the muscle cell differentiation program in vitro. We further investigate known transcriptional repressors for terminal muscle differentiation ZBTB38, Bhlhe41 and Id1. Due to their identified roles for terminal muscle differentiation, we hypothesize that accumulation of these potential cullin E3-ligase substrates may be partially responsible for the observed phenotype. MLN4924 is currently undergoing clinical trials in cancer patients, and our experiments highlight concerns on the homeostasis and regenerative capacity of muscles in these patients who often experience cachexia.

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Section: Discussionmentioning

confidence: 99%

Cullin E3 Ligase Activity Is Required for Myoblast Differentiation

Blondelle

Shapiro

Domenighetti

et al. 2017

Journal of Molecular Biology

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“…Pirfenidone (250 mg kg −1 day −1 ) or vehicle (control) was orally administered daily. After 1 day of the first pirfenidone treatment, CTX was injected into gastrocnemius with 100μl of 10μM (Qiu et al., 2016). Then, the gastrocnemius was isolated on Day 2, Day 6, and Day 12 after CTX injection to analyze the tissue morphology.…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development

Zhang

et al. 2018

Aging Cell

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SummarySatellite cells play a key role in the aging, generation, and damage repair of skeletal muscle. The molecular mechanism of satellite cells in these processes remains largely unknown. This study systematically investigated for the first time the characteristics of mouse satellite cells at ten different ages. Results indicated that the number and differentiation capacity of satellite cells decreased with age during skeletal muscle development. Transcriptome analysis revealed that 2,907 genes were differentially expressed at six time points at postnatal stage. WGCNA and GO analysis indicated that 1,739 of the 2,907 DEGs were mainly involved in skeletal muscle development processes. Moreover, the results of WGCNA and protein interaction analysis demonstrated that Tgfβ2, Wnt9a, and Fgfr4 were the key genes responsible for the differentiation of satellite cells. Functional analysis showed that TGFβ2 and WNT9a inhibited, whereas FGFR4 promoted the differentiation of satellite cells. Furthermore, each two of them had a regulatory relationship at the protein level. In vivo study also confirmed that TGFβ2 could regulate the regeneration of skeletal muscle, as well as the expression of WNT9a and FGFR4. Therefore, we concluded that the synergistic effects of TGFβ2, WNT9a, and FGFR4 were responsible for attenuating of the differentiation of aging satellite cells during skeletal muscle development. This study provided new insights into the molecular mechanism of satellite cell development. The target genes and signaling pathways investigated in this study would be useful for improving the muscle growth of livestock or treating muscle diseases in clinical settings.

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“…Although new muscle fiber formation can compensate for skeletal muscle loss, the regenerative capacity of skeletal muscle decreases with aging . Myogenesis, the process by which myoblasts proliferate, differentiate into myocytes, and fuse into multinucleated myotubes, is a crucial event for postnatal muscle growth and regeneration . Therefore, the MyoD luciferase system was selected to identify natural materials that may aid in maintaining skeletal muscle mass.…”

Section: Discussionmentioning

confidence: 99%

Hydrangea serrata Tea Enhances Running Endurance and Skeletal Muscle Mass

Jang

Ahn

Son

et al. 2019

Molecular Nutrition Food Res

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Scope Skeletal muscle mass and quality can be negatively affected by aging, inactivity, and disease, while a loss of muscle mass is associated with chronic disease status, falls, and mortality. We investigate the effects of Hydrangea serrata on skeletal muscle mass and function, along with the underlying mechanisms. Methods and results H. serrata, identified through MyoD transcription activity screening, increases myogenic differentiation via Akt and p38. C57BL/6 mice are fed a 0.25% or 0.5% H. serrata diet for 8 weeks. H. serrata increased treadmill running distance and maximum speed, as well as skeletal muscle mass. H. serrata promotes the expression of myosin heavy chain 1 (MHC1) and MHC2A but not MHC2B. H. serrata also upregulates the protein expression of peroxisome proliferator‐activated receptor δ (PPARδ) and mitochondrial complexes, and enhances citrate synthase and mitochondrial complex І activity. Transforming growth factor‐β (TGF‐β), myostatin, and growth differentiation factor 11 (GDF11) are attenuated by H. serrata, together with associated downstream signaling factors including phospho‐Smad3 and NADPH oxidase 4 (NOX4). Conclusion H. serrata enhances exercise endurance by upregulating PPARδ and downregulating TGF‐β, myostatin, and GDF11. H. serrata is a potential candidate for the development of functional food to maintain skeletal muscle mass and function.

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MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis

Cited by 47 publications

References 57 publications

Cullin E3 Ligase Activity Is Required for Myoblast Differentiation

Cullin E3 Ligase Activity Is Required for Myoblast Differentiation

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development

Hydrangea serrata Tea Enhances Running Endurance and Skeletal Muscle Mass

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