MicroRNA-16 participates in the cell cycle alteration of HepG2 cells induced by MC-LR

Feng, Yiyi; Chen, Xi; Ding, Weikai; Ma, Jing; Zhang, Bangjun; Li, Xiaoyü

doi:10.1016/j.ecoenv.2020.110295

Cited by 14 publications

(3 citation statements)

References 53 publications

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“…The results were represented as mean ± the standard error of the mean (SEM). Significant differences between the treatments and the control group were calculated by one-way ANOVA [ 24 ]. In addition, Dunnett’s test was used in post hoc pairwise comparisons between the control group and the [C8mim]Cl exposure group.…”

Section: Methodsmentioning

confidence: 99%

Effect of Acute Exposure to the Ionic Liquid 1-Methyl-3-octylimidazolium Chloride on the Embryonic Development and Larval Thyroid System of Zebrafish

Ding

Chen

Sultan

et al. 2022

Animals

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Previous studies have shown that ILs can induce toxicity in animals, plants, and cells. However, the effect of imidazolium-based ILs on the hypothalamus–pituitary–thyroid (HPT) axis of fish remains unknown. The present study aimed to evaluate the acute effect of [C8mim]Cl on the embryonic development and thyroid-controlled internal secretion system of zebrafish by determining the thyroid hormone level and the expression of HPT-related genes. The results obtained for embryonic developmental toxicity showed the survival rate, heart beats, and body length of fish had decreased 96 h after exposure to [C8mim]Cl, but the hatching rate had increased by the 48 h time point. The transcription levels of HTP-related genes showed that the genes dio3, tg, ttr, tsh, trhrα, trhrβ, trhr2, and tpo were up-regulated, while the expression levels of dio1, trh, tshr, and nis were significantly suppressed. Furthermore, we found that exposure to [C8mim]Cl induced an alteration in the levels of thyroid hormones that increased the T3 but decreased the T4 content. In conclusion, our study indicated that acute exposure to [C8mim]Cl altered the expression of HTP-related genes and disturbed the thyroid hormone level, suggesting that the ionic liquid [C8mim]Cl might pose an aquatic environmental threat to fish.

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Section: Methodsmentioning

confidence: 99%

Effect of Acute Exposure to the Ionic Liquid 1-Methyl-3-octylimidazolium Chloride on the Embryonic Development and Larval Thyroid System of Zebrafish

Ding

Chen

Sultan

et al. 2022

Animals

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“…The use of MC-contaminated water in a hemodialysis unit in Brazil resulted in the death of at least 50 patients in 1998 [ 18 ]. As demonstrated by Feng et al, low-dose exposures to MC-LR increased the proliferation of human hepatocellular carcinoma cells [ 19 ]. Human intestinal epithelial cell lines are affected by MC-LR through a complex sequence of events, including the inhibition of PP2A activity, the activation of the P53 protein, and the production of reactive oxygen species.…”

Section: Introductionmentioning

confidence: 99%

Microcystins in Water: Detection, Microbial Degradation Strategies, and Mechanisms

Liu

et al. 2022

IJERPH

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Microcystins are secondary metabolites produced by some cyanobacteria, a class of cyclic heptapeptide toxins that are stable in the environment. Microcystins can create a variety of adverse health effects in humans, animals, and plants through contaminated water. Effective methods to degrade them are required. Microorganisms are considered to be a promising method to degrade microcystins due to their high efficiency, low cost, and environmental friendliness. This review focuses on perspectives on the frontiers of microcystin biodegradation. It has been reported that bacteria and fungi play an important contribution to degradation. Analysis of the biodegradation mechanism and pathway is an important part of the research. Microcystin biodegradation has been extensively studied in the existing research. This review provides an overview of (1) pollution assessment strategies and hazards of microcystins in water bodies and (2) the important contributions of various bacteria and fungi in the biodegradation of microcystins and their degradation mechanisms, including mlr gene-induced (gene cluster expressing microcystinase) degradation. The application of biodegradable technology still needs development. Further, a robust regulatory oversight is required to monitor and minimize MC contamination. This review aims to provide more references regarding the detection and removal of microcystins in aqueous environments and to promote the application of biodegradation techniques for the purification of microcystin-contaminated water.

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“…MC-LR is toxic to liver cells and has been confirmed in many studies to promote abnormal hepatocyte proliferation, invasion and migration, [16][17][18][19] and is also related to apoptosis and cell cycle changes. 20,21 In addition, long-term exposure to MC-LR can induce human hepatitis and even hepatocellular carcinoma. 22 However, the mechanism by which MC-LR is involved in the pathogenesis of hepatocellular carcinoma is still unclear.…”

Section: Introductionmentioning

confidence: 99%

The Abnormal Proliferation of Hepatocytes is Associated with MC-LR and C-Terminal Truncated HBX Synergistic Disturbance of the Redox Balance

Cai¹,

Mei²,

Zhang³

et al. 2022

JHC

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Background: Microcystin-LR (MC-LR) and hepatitis B virus (HBV) are associated with hepatocellular carcinoma (HCC). However, the concentrations of MC-LR in drinking water and the synergistic effect of MC-LR and HBV on hepatocellular carcinogenesis through their disturbance of redox balance have not been fully elucidated. Methods: We measured the MC-LR concentrations in 168 drinking water samples of areas with a high incidence of HCC. The relationships between MC-LR and both redox status and liver diseases in 177 local residents were analyzed. The hepatoma cell line HepG2 transfected with C-terminal truncated hepatitis B virus X gene (Ct-HBX) were treated with MC-LR. Reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione (GSH) and malondialdehyde (MDA) were measured. Cell proliferation, migration, invasion, and apoptosis were assessed with cell activity assays, scratch and transwell assays, and flow cytometry, respectively. The mRNA and protein expression-related redox status genes were analyzed with qPCR and Western blotting. Results:The average concentration of MC-LR in well water, river water and reservoir water were 57.55 ng/L, 76.74 ng/L and 132.86 ng/L respectively, and the differences were statistically significant (P < 0.05). The MC-LR levels in drinking water were correlated with liver health status, including hepatitis, clonorchiasis, glutamic pyruvic transaminase abnormalities and hepatitis B surface antigen carriage (all P values < 0.05). The serum MDA increased in subjects who drank reservoir water and were infected with HBV (P < 0.05). In the cell experiment, ROS increased when Ct-HBX-transfected HepG2 cells were treated with MC-LR, followed by a decrease in SOD and GSH and an increase in MDA. MC-LR combined with Ct-HBX promoted the proliferation, migration and invasion of HepG2 cells, upregulated the mRNA and protein expression of MAOA gene, and downregulated UCP2 and GPX1 genes. Conclusion: MC-LR and HBV may synergistically affect redox status and play an important role in hepatocarcinoma genesis.

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MicroRNA-16 participates in the cell cycle alteration of HepG2 cells induced by MC-LR

Cited by 14 publications

References 53 publications

Effect of Acute Exposure to the Ionic Liquid 1-Methyl-3-octylimidazolium Chloride on the Embryonic Development and Larval Thyroid System of Zebrafish

Effect of Acute Exposure to the Ionic Liquid 1-Methyl-3-octylimidazolium Chloride on the Embryonic Development and Larval Thyroid System of Zebrafish

Microcystins in Water: Detection, Microbial Degradation Strategies, and Mechanisms

The Abnormal Proliferation of Hepatocytes is Associated with MC-LR and C-Terminal Truncated HBX Synergistic Disturbance of the Redox Balance

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