MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells

Wang, Fang; Wang, Wendi; Lu, Lina; Xie, Youhua; Yan, Junli; Chen, Yuhong; Chen, Di; Gan, Lu; Si, Jing; Mao, Aihong

doi:10.3892/or.2020.7682

Cited by 24 publications

(11 citation statements)

References 50 publications

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“…Studies have pointed out that upregulating the expression of miR-16-5p can inhibit the proliferation and metastasis of liver cancer cells [ 12 ]. miR-16-5p inhibits the survival of prostate cancer cells by targeting protein kinase 3 (AKT3), regulates cell cycle distribution, and induces apoptosis [ 13 ]. In addition, miR-16-5p also mediates the inhibitory effect of knockdown Linc0021 on the biological behavior of nonsmall cell lung cancer [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Effect and Mechanism of lncRNA CERS6-AS1 on the Biological Behavior of Prostate Cancer Cell

Huang

Zhou

2022

Applied Bionics and Biomechanics

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Objective. To investigate the effect of long noncoding RNA (lncRNA) CERS6 antisense RNA1 (CERS6-AS1) on the biological behavior of prostate cancer cells DU145 and its mechanism. Methods. RT-PCR was used to detect the relative level of CERS6-AS1 and miR-16-5p in prostate cancer tissues, adjacent tissues, prostate cancer cells DU145, and human normal prostate epithelial cells RWPE-1. DU145 cells were divided into control group, si-CERS6-AS1 group, si-NC group, miR-16-5p mimic group, miR-NC group, and si-CERS6-AS1+miR-16-5p inhibitor group. And CCK-8 method and colony formation test was applied to detect cell proliferation ability, flow cytometry was selected to calculate cell apoptosis, and scratch healing test was employed to assess cell migration ability. Western blot was determined to detect high mobility protein A2 (HMGA2) expression. RT-PCR and dual-luciferase reporter experiments were used to analyze the targeting relationship among CERS6-AS1, miR-16-5p, and HMGA2. Results. Compared with the adjacent tissues, the relative level of CERS6-AS1 in prostate cancer tissue was increased ( P < 0.05 ), and the relative level of miR-16-5p was decreased ( P < 0.05 ). Compared with RWPE-1 cells, the relative level of CERS6-AS1 in DU145 cells was increased ( P < 0.05 ), and the relative level of miR-16-5p was decreased ( P < 0.05 ). Compared with the control group and the si-NC group, the HMGA2 protein expression, the colony formation number, and the scratch healing rate of DU145 cells in the si-CERS6-AS1 group and the miR-16-5p mimic group were reduced ( P < 0.05 ), and the relative level of miR-16-5p and the proliferation inhibition rate and apoptosis were increased ( P < 0.05 ). miR-16-5p is specifically bound to CERS6-AS1 and HMGA2, respectively. Compared with the si-CERS6-AS1 group, the HMGA2 protein expression, the colony formation number, and the scratch healing rate of DU145 cells in the si-CERS6-AS1+miR-16-5p inhibitor group were increased ( P < 0.05 ), and the cell proliferation inhibition rate and apoptosis rate were reduced ( P < 0.05 ). Conclusion. Silencing CERS6-AS1 can inhibit the proliferation and migration of prostate cancer cell DU145 and induce cell apoptosis, the mechanism is related to the regulation of the miR-16-5p/HMGA2 axis.

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Section: Discussionmentioning

confidence: 99%

Effect and Mechanism of lncRNA CERS6-AS1 on the Biological Behavior of Prostate Cancer Cell

Huang

Zhou

2022

Applied Bionics and Biomechanics

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“…On the other hand, miR 221 and miR 222 act on different kinase-dependent cyclin inhibitors such as p27 kip1 in nasopharyngeal carcinomas, which causes the hyperactivation of cyclins with increased tumor growth [ 44 ]. Similarly, other works have demonstrated that miR-16, a product downregulated in prostate cancer cells, can directly target AKT3, thus ameliorating cell cycle dysregulation and inducing cell apoptosis [ 45 ]. According to recent reviews on penile cancer [ 46 ], an overexpression of oncogenic miRNAs (miR-223-3p, miR-107 and miR-21-5p) was directly involved in PTEN suppression in addition to alterations in the mitogen-activated protein kinase pathway MAPK (ERK1/ERK2), whereas miR-145 downregulation drove to marked alterations in oncogenic genes such as c-myc.…”

Section: Role Of Micrornas In Cell Cyclementioning

confidence: 95%

An Overview of the Role of MicroRNAs on Carcinogenesis: A Focus on Cell Cycle, Angiogenesis and Metastasis

Pekarek

Torres-Carranza

Fraile-Martínez

et al. 2023

IJMS

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In recent years, the importance of epigenetic markers in the carcinogenesis of different malignant neoplasms has been demonstrated, also demonstrating their utility for understanding metastatic spread and tumor progression in cancer patients. Among the different biomarkers, microRNAs represent a set of non-coding RNAs that regulate gene expression, having been involved in a wide variety of neoplasia acting in different oncogenic pathways. Both the overexpression and downregulation of microRNAs represent a complex interaction with various genes whose ultimate consequence is increased cell proliferation, tumor invasion and interaction with various driver markers. It should be noted that in current clinical practice, even though the combination of different microRNAs has been shown to be useful by different authors at diagnostic and prognostic levels, there are no diagnostic kits that can be used for the initial approach or to assess recurrences of oncological diseases. Previous works have cited microRNAs as having a critical role in several carcinogenic mechanisms, ranging from cell cycle alterations to angiogenesis and mechanisms of distant metastatic dissemination. Indeed, the overexpression or downregulation of specific microRNAs seem to be tightly involved in the modulation of various components related to these processes. For instance, cyclins and cyclin-dependent kinases, transcription factors, signaling molecules and angiogenic/antiangiogenic products, among others, have been recognized as specific targets of microRNAs in different types of cancer. Therefore, the purpose of this article is to describe the main implications of different microRNAs in cell cycle alterations, metastasis and angiogenesis, trying to summarize their involvement in carcinogenesis.

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“…Through bioinformatic analyses, these authors predicted its interaction with targets such as WEE1, Chk1, and CDC27 ( 66 ). miR-16-5p had been previously observed to inhibit proliferation in prostate ( 106 ) and breast ( 107 ) cancers by targeting AKT.…”

Section: Ncrnas Involved In Cell Cyclementioning

confidence: 99%

Non-Coding RNAs Associated With Radioresistance in Triple-Negative Breast Cancer

et al. 2021

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The resistance that Triple-Negative Breast Cancer (TNBC), the most aggressive breast cancer subtype, develops against radiotherapy is a complex phenomenon involving several regulators of cell metabolism and gene expression; understanding it is the only way to overcome it. We focused this review on the contribution of the two leading classes of regulatory non-coding RNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), against ionizing radiation-based therapies. We found that these regulatory RNAs are mainly associated with DNA damage response, cell death, and cell cycle regulation, although they regulate other processes like cell signaling and metabolism. Several regulatory RNAs regulate multiple pathways simultaneously, such as miR-139-5p, the miR-15 family, and the lncRNA HOTAIR. On the other hand, proteins such as CHK1 and WEE1 are targeted by several regulatory RNAs simultaneously. Interestingly, the study of miRNA/lncRNA/mRNA regulation axes increases, opening new avenues for understanding radioresistance. Many of the miRNAs and lncRNAs that we reviewed here can be used as molecular markers or targeted by upcoming therapeutic options, undoubtedly contributing to a better prognosis for TNBC patients.

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MicroRNA‑16‑5p regulates cell survival, cell cycle and apoptosis by targeting AKT3 in prostate cancer cells

Cited by 24 publications

References 50 publications

Effect and Mechanism of lncRNA CERS6-AS1 on the Biological Behavior of Prostate Cancer Cell

Effect and Mechanism of lncRNA CERS6-AS1 on the Biological Behavior of Prostate Cancer Cell

An Overview of the Role of MicroRNAs on Carcinogenesis: A Focus on Cell Cycle, Angiogenesis and Metastasis

Non-Coding RNAs Associated With Radioresistance in Triple-Negative Breast Cancer

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