MicroRNA-15a tissue expression is a prognostic marker for survival in patients with clear cell renal cell carcinoma

Mytsyk, Yulian; Borys, Yuriy; Tumanovska, Lesya V.; Stroy, Dmytro; Kucher, Askold; Gazdíková, Katarína; Rodrigo, Luı́s; Kružliak, Peter; Prosecký, Robert; Urdzík, Peter; Dosenko, Victor E.

doi:10.1007/s10238-019-00574-7

Cited by 11 publications

(5 citation statements)

References 40 publications

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“…Others studies reported that over-expression of miR-15a was strongly associated with poor histological prognostic features of ccRCC, suggesting miR-15a as a potential prognostic molecular biomarker [ 12 ]. Target genes of miR-576 ( CUL3 and RAC1 ) have been identified to be involved in the regulation of multiple cancer-related biological pathways, and the target genes of miR-616 ( ASB13 and FBXW2 ) have been reported to be associated with the development of other cancers.…”

Section: Introductionmentioning

confidence: 99%

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Arance

Ramírez

Rubio-Roldán

et al. 2021

Cancers

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Here, the role of non-invasive biomarkers in liquid biopsy was evaluated, mainly in exosomes and mitochondrial DNA (mtDNA) as promising, novel, and stable biomarkers for renal cell carcinoma (RCC). A total of 140 fractions (named from B to F) obtained by ultracentrifugations of whole blood samples from 28 individuals (13 patients and 15 controls) were included. Nanoparticle Tracking Analysis (NTA) was conducted to characterized exosomal fraction. Subsequently, an analysis of digital PCR (dPCR) using the QuantStudio™ 3D Digital PCR platform was performed and the quantification of mtDNA copy number by QuantStudioTM 12K Flex Real-Time PCR System (qPCR) was developed. Moreover, Next Generation Sequencing (NGS) analyses were included using MiSeq system (Illumina, San Diego, CA, USA). An F fraction, which contains all exosome data and all mitochondrial markers, was identified in dPCR and qPCR with statistically significant power (adjusted p values ≤ 0.03) when comparing cases and controls. Moreover, present analysis in mtDNA showed a relevant significance in RCC aggressiveness. To sum up, this is the first time a relation between exosomal mtDNA markers and clinical management of RCC is analyzed. We suggest a promising strategy for future liquid biopsy RCC analysis, although more analysis should be performed prior to application in routine clinical practice.

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Section: Introductionmentioning

confidence: 99%

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Arance

Ramírez

Rubio-Roldán

et al. 2021

Cancers

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“…Mytsyk et al studied the expression of miR-15a in the urine of RCC patients, and found that compared with benign renal tumor patients and healthy control group, the expression of miR-15a in RCC patients before operation was significantly upregulated, and that in RCC patients after operation was significantly downregulated, suggesting that the expression of miR-15a in the urine can be used as a biomarker for RCC diagnosis. Their subsequent study confirmed that the expression of miR-15a in RCC patients' tumor tissue was significantly upregulated compared with the healthy control group, and the over expression of miR-15a was closely related to the poor prognosis of RCC, indicating that the expression of miR-15a in the tissue can be used as a molecular marker of RCC prognosis [10].…”

Section: Upregulated Mirnas In Rccmentioning

confidence: 79%

Abnormal Expression of miRNAs in RCC

2020

2020 2nd International Symposium on the Frontiers of Biotechnology and Bioengineering (FBB 2020)

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Renal cell carcinoma (RCC) is one of the most common malignant tumors of urinary system, accounting for about 3% of human malignant tumors, which is the tenth most common cancer in the world. However, there is a lack of accurate biomarkers for the diagnosis of RCC, and the mechanism of the occurrence and development of RCC is not clear. In recent years, many studies have focused on the abnormal expression of miRNAs in RCC. Studying the abnormal expression of miRNAs in RCC is helpful to understand the mechanism of occurrence and development of RCC, and to provide new ideas for the development of potential biomarkers and therapeutic targets of RCC.

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“…Similarly, miR-15a was upregulated in RCC patients and associated with poor prognosis of RCC patients. 24 Overexpression of miR-15a could promote cervical cancer cell proliferation and depress cell apoptosis to aggravate cervical cancer. 25 In the presented research, the experimental evidence displayed that upregulation Vimentin, a mesenchymal marker, and the reduced level of Ecadherin, an epithelial marker, indicated the facilitation of EMT activity.…”

Section: Discussionmentioning

confidence: 99%

Exosomal microRNA‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2/PI3K/AKT axis

Lin

et al. 2021

The Kaohsiung J of Med Scie

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Accumulating studies have indicated that exosomal microRNAs (miRNAs/miRs) can mediate clear cell renal cell carcinoma (ccRCC) at the early stage, but the mechanisms remain to be specified. Here, we investigated the mechanism of exosomal miR-15a in ccRCC. After successful isolation of exosomes from RCC cells, we found that miR-15a was upregulated in ccRCC cells. Moreover, upregulation of miR-15a by pre-miR-15a promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition of ccRCC cells. A luciferase assay revealed that B-cell translocation gene 2 (BTG2) was a target gene of miR-15a and negatively correlated with miR-15a expression. BTG2 was poorly expressed in ccRCC, which reduced the proliferation of ccRCC cells. In addition, overexpression of BTG2 could reverse the promotive effects of miR-15a on ccRCC. Furthermore, BTG2 reduced PI3K/AKT pathway activity. Our results collectively indicated that exosomal miR-15a from RCC cells accelerated cell viability by downregulating BTG2 and promoting the activity of the PI3K/AKT signaling pathway. We demonstrated a novel mechanism by which exosomal miR-15a exerted pro-proliferatory effects on ccRCC, highlighting the potential of exosomal miR-15a as a target for ccRCC prognosis.

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MicroRNA-15a tissue expression is a prognostic marker for survival in patients with clear cell renal cell carcinoma

Cited by 11 publications

References 40 publications

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Determination of Exosome Mitochondrial DNA as a Biomarker of Renal Cancer Aggressiveness

Abnormal Expression of miRNAs in RCC

Exosomal microRNA‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2/PI3K/AKT axis

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