MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

Mytsyk, Yulian; Dosenko, Viktor Е.; Borys, Yuriy; Kucher, Askold; Gazdíková, Katarína; Büsselberg, Dietrich; Čaprnda, Martin; Kružliak, Peter; Farooqı, Ammad Ahmad; Manyuk, L.

doi:10.1007/s11255-018-1841-x

Cited by 45 publications

(43 citation statements)

References 26 publications

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“…Besides proteins, micro RNAs (miRs) are a promising candidate for biomarkers. As an example, we have identified miR-15a to be overexpressed in RCCs tissue [ 3 ] and has recently also be shown by Mytsyk et al [ 4 ].…”

Section: Introductionmentioning

confidence: 83%

Beyond the 3′UTR binding-microRNA-induced protein truncation via DNA binding

et al. 2018

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Here, we present a miR mechanism which is active in the nucleus and is essential for the production of intron included, C-terminal truncated and biologically active proteins, like e.g. Vim3. We exemplified this mechanism by miRs, miR-15a and miR-498, which are overexpressed in clear cell renal carcinoma or oncocytoma.Both miRs directly interact with DNA in an intronic region, leading to transcriptional stop, and therefore repress the full length version of the pre-mRNA, resulting in intron included truncated proteins (Mxi-2 and Vim3). A computational survey shows that this miR:DNA interactions mechanism may be generally involved in regulating the human transcriptome, with putative interaction sites in intronic regions for over 1000 genes.In this work, an entirely new mechanism is revealed how miRs can repress full length protein translation, resulting in C-terminal truncated proteins.

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Section: Introductionmentioning

confidence: 83%

Beyond the 3′UTR binding-microRNA-induced protein truncation via DNA binding

et al. 2018

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“…The idea of testing miRNAs in the urine for ccRCC diagnosis is not new. Von Brandenstein et al 17 and Mytsyk et al 18 found high levels of miR-15a in the urine of patients with RCC. Li et al 19 found that urinary levels of miR-210 were significantly higher in patients with ccRCC than in HSs, with sensitivity, specificity, and AUC equal to 58%, 80%, and 0.76, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs are stable and present in bodily fluids (e.g., blood plasma, urine, saliva, and semen) within microvesicles, apoptotic bodies, or membrane-free carriers 8,14 , which make these RNAs promising biomarkers for acute diseases 15 and cancer diagnosis and prognosis 10,16 . Although many studies have evaluated miRNA expression in tissues and serum of patients with ccRCC, only a few have investigated the potential role of miRNAs as urinary biomarkers [17][18][19][20][21] .…”

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confidence: 99%

Detection of urinary miRNAs for diagnosis of clear cell renal cell carcinoma

Cochetti

Cari

Nocentini

et al. 2020

Sci Rep

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The lack of symptoms at the early stages of clear cell renal cell carcinoma (ccRCC) allows the tumour to metastasize, leading to a dramatic reduction in patient survival. Therefore, we studied and set up a method based on urinary microRNAs (miRNAs) for the diagnosis of ccRCC. First, miRNA expression in ccRCC specimens and kidney tissues from healthy subjects (HSs) was investigated through analysis of data banks and validated by comparing expression of miRNAs in ccRCC and adjacent non-cancerous kidney tissue specimens by RT-qPCR. Subsequently, we developed an algorithm to establish which miRNAs are more likely to be found in the urine of ccRCC patients that indicated miR-122, miR-1271, and miR-15b as potential interesting markers. The evaluation of their levels and three internal controls in the urine of 13 patients and 14 HSs resulted in the development of a score (7p-urinary score) to evaluate the presence of ccRCC in patients. The resulting area under the Receiver Operating Characteristic (ROC) curve, sensitivity, and specificity were equal to 0.96, 100% (95% CI 75–100%), and 86% (95% CI 57–98%), respectively. In conclusion, our study provides a proof of concept that combining the expression values of some urinary miRNAs might be useful in the diagnosis of ccRCC.

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“…Remarkably, both miRs also distinguished benign lesions from normal with 75.0% sensitivity and 82.8% [82]. Additionally, urinary miR-15a expression levels, evaluated in 67 RCT patients and 15 AC, detected malignancy with 98.1% sensitivity and 100% specificity [83]. A summary of these and other studies is depicted in Table 2.…”

Section: Liquid Biopsiesmentioning

confidence: 70%

Renal Cell Tumors: Uncovering the Biomarker Potential of ncRNAs

Outeiro-Pinho

Barros‐Silva

Correia

et al. 2020

Cancers

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Renal cell tumors (RCT) remain as one of the most common and lethal urological tumors worldwide. Discrimination between (1) benign and malignant disease, (2) indolent and aggressive tumors, and (3) patient responsiveness to a specific therapy is of major clinical importance, allowing for a more efficient patient management. Nonetheless, currently available tools provide limited information and novel strategies are needed. Over the years, a putative role of non-coding RNAs (ncRNAs) as disease biomarkers has gained relevance and is now one of the most prolific fields in biological sciences. Herein, we extensively sought the most significant reports on ncRNAs as potential RCTs’ diagnostic, prognostic, predictive, and monitoring biomarkers. We could conclude that ncRNAs, either alone or in combination with currently used clinical and pathological parameters, might represent key elements to improve patient management, potentiating the implementation of precision medicine. Nevertheless, most ncRNA biomarkers require large-scale validation studies, prior to clinical implementation.

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MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

Abstract: MiR-15a expression measured in urine may be used as diagnostic molecular biomarker for RCC.

Cited by 45 publications

References 26 publications

Beyond the 3′UTR binding-microRNA-induced protein truncation via DNA binding

Beyond the 3′UTR binding-microRNA-induced protein truncation via DNA binding

Detection of urinary miRNAs for diagnosis of clear cell renal cell carcinoma

Renal Cell Tumors: Uncovering the Biomarker Potential of ncRNAs

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