MicroRNA-155 is a potential molecular marker of natural killer/T-cell lymphoma

Abstract

Natural killer/T-cell lymphoma (NKTCL) is characterized by its highly aggressive nature and rapid progression. MicroRNAs (miRNAs) play key roles in the development of NKTCL. We utilized next-generation Solexa high-throughput sequencing to compare miRNA expression in the SNK-6 and YTS NKTCL cell lines with expression in normal NK cells. We found that 195 miRNAs were upregulated in the SNK-6 cells and 286 miRNAs were upregulated in the YTS cells. Based on those results, we selected six miRNAs, including miRNA-155, and confirmed their expression using real-time polymerase chain reaction. Expression of miRNA-155 was higher in SNK-6 and YKS cells than in normal NK cells. We next determined the levels of miRNA-155 in the serum of healthy individuals and NKTCL patients, and correlated its expression with clinical parameters and inflammatory factors detected using enzyme-linked immunoabsorbent assays. Levels of miRNA-155 were higher in NKTCL patients’ serum than in serum from healthy individuals. miRNA-155 expression was higher in patients with stable or progressive disease (SD+PD) than in those with partial or complete remission (PR+CR). While further studies are needed to clarify the underlying molecular mechanisms, it appears miRNA-155 may be a molecular marker of NKTCL.

Epigenetic aberrations in natural killer/T-cell lymphoma: diagnostic, prognostic and therapeutic implications

“…87 Zhang and colleagues observed significantly higher miR-155 levels in the serum of NKTCL patients compared with the levels in healthy individuals. 70 More importantly, serum miR-155 levels were higher in patients with stable or progressive disease compared with those in partial or complete remission. 70 Two distinct studies reported an inverse relationship between ASNS (asparagine synthetase) expression level and response to asparaginasebased chemotherapy in NKTCL cell lines and patient samples.…”

Whole transcriptome analysis reveals dysregulated oncogenic lncRNAs in natural killer/T-cell lymphoma and establishes MIR155HG as a target of PRDM1

“…62 In addition, mir155 overexpression led to the expansion and activation of NK-cells in vivo. 63 Intriguingly, mir155 has recently been reported to be overexpressed in NKTCL, 64 and its overexpression may account for the activation of AKT pathway observed previously by DNA microarray in NKTCL cases. 58 These observations suggest that loss-of-function of PRDM1 may, at least in part, be responsible for mir155 overexpression in NKTCLs.…”

In situ Study Unravels Bio‐Nanomechanical Behavior in a Magnetic Bacterial Nano‐cellulose (MBNC) Hydrogel for Neuro‐Endovascular Reconstruction

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