MicroRNA-150 promote apoptosis of ovine ovarian granulosa cells by targeting STAR gene

Zhou, Rongyan; Miao, Yanping; Li, Yimeng; Li, Xiangyun; Xi, Jianzhong; Zhang, Zhenhong

doi:10.1016/j.theriogenology.2019.01.003

Cited by 23 publications

(11 citation statements)

References 18 publications

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“…STAR is the rate‐limiting enzyme for steroid synthesis. Moreover, our present study had demonstrated that miR‐150 promoted GCs apoptosis by targeting STAR (Zhou et al., 2019). In this present study, both miR‐150 and miR‐194 targeted signal transducers and activators of transcription 1 ( STAT1 ).…”

Section: Discussionmentioning

confidence: 62%

“…Besides, we identified 19 differentially expressed miRNAs in ovarian follicular and luteal phase of the Turpan black sheep. Among them, miR‐150 is putatively involved in cattle follicular atresia (Donadeu et al., 2017) and promotes ovine granulosa cell apoptosis (Zhou et al., 2019). MiR‐27a affects the maturation of oocyte (Kim et al., 2019), promotes human granulosa cell apoptosis by targeting SMAD5 (Nie et al., 2015) and may be related to premature ovarian insufficiency (POI) (Guo et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

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Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

Song

Yue

et al. 2020

Reprod Domestic Animals

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To investigate the regulatory mechanism of the follicular–luteal phase transition in Turpan black sheep (Ovis aries), the genome‐wide expression patterns of microRNAs (miRNAs) and genes were investigated in ovaries of six sheep (3 years and single lamb with 3 consecutive births) during follicular and luteal phases of the oestrous cycle. Bioinformatic analysis was used to screen potential miRNAs and genes related to Turpan black sheep ovarian function. RT‐qPCR was used to validate the sequencing results. In total, we identified 139 known and 71 novel miRNAs in the two phases with miRNA‐seq, and a total of 19 miRNAs were significantly differentially expressed, of which 7 were up‐regulated and 12 were down‐regulated in the follicular phase compared with luteal phase. A total of 150 genes were significantly differentially expressed, including 63 up‐regulated and 87 down‐regulated in the follicular phase compared with the luteal phase by RNA‐seq data analysis. Those DEGs were significantly enriched in 103 GO terms and several KEGG pathways, including metabolic pathway, ovarian steroidogenesis, steroid hormone biosynthesis and oestrogen signalling pathway. In addition, we created a miRNA–mRNA regulatory network to further elucidate the mechanism of follicular–luteal transition. Finally, we identified key miRNAs and genes including miR‐143, miR‐99a, miR‐150, miR‐27a, miR‐125b, STAR, STAT1, which might play crucial roles in reproductive hormone biosynthesis and follicular development. The miRNA–mRNA interactive network clearly illustrates molecular basis involving in follicular–luteal transition.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

Song

Yue

et al. 2020

Reprod Domestic Animals

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“…Previous reports showed that the miR-150 could degrade or block mRNA translation through binding to the 3’-untranslated region of target gene [49]. The miR-150 also promoted sheep ovarian granulosa cells apoptosis through inhibition of steroidogenic acute regulatory (STAR) protein expression [50]. The miR-370 inhibited tumor growth with decreased expression in non-small cell lung cancer, while it was found to be oncogene with overexpression in prostate cancer [51].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Profile in Peripheral Blood Lymphocytes of Sheep Vaccinated with Nigeria 75/1 Peste Des Petits Ruminants Virus

Yang

Qin

Meng

et al. 2019

Viruses

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Peste des petits ruminants (PPR) is one of the highly contagious transboundary viral diseases of small ruminants. Host microRNA (miRNA) expression patterns may change in response to virus infection, and it mainly works as a post-transcriptional moderator in gene expression and affects viral pathogenesis and replication. In this study, the change of miRNA expression profile in peripheral blood lymphocyte (PBMC) from sheep inoculated with PPR vaccine virus in vivo as well as primary sheep testicular (ST) cells inoculated with PPR vaccine virus in vitro were determined via deep sequencing technology. In PBMC cells, 373 and 115 differentially expressed miRNAs (DEmiRNAs) were identified 3 days and 5 days post inoculated (dpi), respectively. While, 575 DEmiRNAs were identified when comparing miRNA profiles on 5 dpi with 3 dpi. Some of the DEmiRNAs were found to change significantly via time-course during PPR vaccine virus inoculated. Similarly, in ST cells, 136 DEmiRNAs were identified at 3 dpi in comparison with mock-inoculation. A total of 12 DEmiRNAs were validated by real-time quantitative PCR (RT-qPCR). The oar-miR-150, oar-miR-370-3p and oar-miR-411b-3p were found common differentially expressed in both PPR vaccine virus-inoculated PBMC cells and ST cells. Targets prediction and functional analysis of the DEmiRNAs uncovered mainly gathering in antigen processing and presentation pathways, protein processing in endoplasmic reticulum pathways and cell adhesion molecules pathways. Our study supplies information about the DEmiRNAs in PPR vaccine virus-inoculated PBMC cells and ST cells, and provides clues for further understanding the function of miRNAs in PPR vaccine virus replication.

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“…Yao et al (2018) demonstrated that miRNA-181b attenuates porcine granulosa cell apoptosis by inhibiting the expression of SMAD7, activating the TGF-β signaling pathway and increasing expression with the transforming growth factor beta receptor (TGFBR1) promoter (Yao et al 2018). Very recently, Zhou et al (2019) provided evidence that miRNA-150 promotes apoptosis of ovine ovarian granulosa cells by targeting the StAR protein gene (STAR gene). Several additional miRNAs have been identified as summarized in Supplementary Table 2 that either modulate ovarian cell proliferation or apoptosis.…”

Section: Journal Of Molecular Endocrinologymentioning

confidence: 99%

“…These include gonadotropin receptors (FSHR, LHCGR), steroidogenic enzymes (CYP19A1), transcription factors (Creb1, Foxl2, LRH-1, SF-1, E2F1, RUNX2, SREBP-1a, SREBP-2), cholesterol transport proteins (StAR) and signaling proteins (HRas, EFNA3, IGF-1, MAP3K8) (Table 2). Likewise, in testicular Leydig cells, miRNA-150 and let-7b target cholesterol transport protein, StAR Xu et al 2011, Pan et al 2015, Yin et al 2012, Dai et al 2013, Kitahara et al 2013, Troppmann et al 2014, Yin et al 2014, Song et al 2015, Du et al 2016b, Huang et al 2016, Wang et al 2016a, Men et al 2017, Wang et al 2017, Tu et al 2019, Zhang et al 2017a, Liu et al 2018, Menon et al 2018, Shukla et al 2018, Zhang et al 2019a, Zhou et al 2019 Testicular Hu et al , Hu et al 2017, Geng et al 2017, Men et al 2017, Gao et al 2018, Ha et al 2018 Creb1, cAMP-responsive element-binding protein1; E2F1, E2F transcription factor 1, FSHR, follicle-stimulating hormone receptor; Foxl2, forkhead Box L2; IGF-1, insulin-like growth factor-1; LHCGR, luteinizing hormone/chorionic gonadotropin receptor; LRBP, luteinizing hormone receptor (LHR) mRNA-binding protein; LRH-1, liver receptor homolog-1; MAP3K1, mitogen-activated protein kinase, kinase, kinase1; MAP3K8, mitogen-activated protein kinase kinase kinase 8; Mecp2, methyl CpG-binding protein; Mta-3, metastasis-associated 3; Nur77, orphan nuclear receptor Nur77 (also known as NR4A1, NGF1B, TR3TIS1, NAK or N10); Nurr1, Nurr1 transcription factor, also called nuclear receptor subfamily 4 group A member 2 (Nr4a2); RBMS1, RNA-binding motif single-stranded interacting protein 1; RUNX2, Runt-related transcription factor also known as core binding factor subunit alpha-1 (CBP-alpha-1); SR-B1, scavenger receptor class B, type 1; SREBP-1a, sterol regulatory element-binding protein1a; SREBP-2, sterol regulatory element-binding prot...…”

Section: Steroidogenic Enzymes and Protein Factors As Potential Targementioning

confidence: 99%

The role of miRNAs in regulating adrenal and gonadal steroidogenesis

Azhar

Dong

Shen

et al. 2020

Journal of Molecular Endocrinology

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miRNAs are endogenous noncoding single-stranded small RNAs of ~22 nucleotides in length that post-transcriptionally repress the expression of their various target genes. They contribute to the regulation of a variety of physiologic processes including embryonic development, differentiation and proliferation, apoptosis, metabolism, hemostasis and inflammation. In addition, aberrant miRNA expression is implicated in the pathogenesis of numerous diseases including cancer, hepatitis, cardiovascular diseases and metabolic diseases. Steroid hormones regulate virtually every aspect of metabolism, and acute and chronic steroid hormone biosynthesis is primarily regulated by tissue-specific trophic hormones involving transcriptional and translational events. In addition, it is becoming increasingly clear that steroidogenic pathways are also subject to post-transcriptional and post-translational regulations including processes such as phosphorylation/dephosphorylation, protein‒protein interactions and regulation by specific miRNAs, although the latter is in its infancy state. Here, we summarize the recent advances in miRNA-mediated regulation of steroidogenesis with emphasis on adrenal and gonadal steroidogenesis.

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MicroRNA-150 promote apoptosis of ovine ovarian granulosa cells by targeting STAR gene

Cited by 23 publications

References 18 publications

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

MicroRNA Expression Profile in Peripheral Blood Lymphocytes of Sheep Vaccinated with Nigeria 75/1 Peste Des Petits Ruminants Virus

The role of miRNAs in regulating adrenal and gonadal steroidogenesis

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