MicroRNA-150 Modulates Adipogenic Differentiation of Adipose-Derived Stem Cells by Targeting Notch3

Abstract

MicroRNAs (miRNAs) influence stem cell functions, including mobilization, proliferation, and differentiation. miR-150 is abundantly expressed in monocytes. Knockdown of miR-150 promotes bone marrow stem cell migration. The role of miR-150 in adipose-derived stem cells (ADSCs) is unclear. In this study, the effects of miR-150 on adipogenic differentiation and proliferation of ADSCs were investigated. ADSCs were isolated from the inguinal adipose tissue of wild-type (WT) and miR-150 knockout (KO) mice and were induced for adipogenic differentiation. The miR-150 level was detected by real-time PCR. ADSCs were transfected by miR-150 or small-interfering RNA (siRNA) of Notch3. MTT assay and colony formation assay were performed in miR-150 knockdown and control ADSCs. Real-time PCR showed that miR-150 was expressed in ADSCs. miR-150 knockdown significantly decreased the capacity of adipogenic differentiation of ADSCs, as compared with their counterparts from WT mice. It is intriguing that the overexpression of miR-150 significantly increased C/EBPα and PPAR-γ expression and lipid formation in ADSCs with adipogenic induction. Overexpression of miR-150 significantly decreased Notch3 expression in ADSCs compared with the control groups. Furthermore, Notch3 inhibition promoted the adipogenic differentiation in ADSCs. miR-150 also suppressed proliferation potential and the expression of Nanog in ADSCs. In summary, this study demonstrates, for the first time, that miR-150 promotes adipogenic differentiation and inhibits proliferation of ADSCs. miR-150 regulates adipogenic differentiation of ADSCs, likely mediated by the downregulation of Notch3.

miR-10a-5p Inhibits the Differentiation of Goat Intramuscular Preadipocytes by Targeting KLF8 in Goats

“…MicroRNAs have been reported to regulate adipogenic differentiation ( Hamam et al, 2015 ; Tang et al, 2017 ; Ai et al, 2019 ; Li et al, 2019 ); however, the role of miR-10a-5p in goat intramuscular adipogenesis has not been reported. The differential expression of miR-10a-5p after GIPC differentiation has been observed by miRNA sequencing technology in our previous study (data not shown).…”

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“…This might be related to the complexity of the regulatory mechanism of cell differentiation. We have known that many proteins 45,46 and a lot of miRNAs 25,[47][48][49][50][51] have been reported to participate in adipogenic differentiation. Similarly, for angiogenesis, both proteins [52][53][54][55][56][57] and miRNAs [58][59][60] played a regulatory role.…”

Epigenetics of ankylosing spondylitis: Recent developments

“…These include: (1) stability; (2) tissue specificity; (3) miRNA expression is related to specific physiological and pathological states; and (4) the ability of miRNA to differentiate, which can improve the accuracy of diagnosis 47 48 apoptosis, 49 differentiation 50 and development 51 . In recent years, with the deepening of research, the mechanism of ncRNA in complex diseases has become a research hotspot.…”