MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma

Xu, Jin; Wang, Zengliang; Liao, Zhichao; Dong, Diansheng; Ma, Xiaofang

doi:10.3892/ol.2017.6393

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…ZEB1 expression is significantly correlated with the lung metastasis of patients with OS (40). Dysregulation of ZEB1 is associated with the aggressive behaviors of OS cells via regulation of numerous pathological processes, including cell proliferation, migration, invasion, metastasis and chemoresistance (41)(42)(43)(44). Thus, miR-708-based therapy targeted against ZEB1 expression may serve as an effective strategy in the treatment of patients with OS.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑708 inhibits the proliferation and invasion of osteosarcoma cells by directly targeting ZEB1

Xiang

Lin

2019

Mol Med Report

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Numerous microRNAs (miRNAs) have been identified as aberrantly expressed in osteosarcoma (OS). miRNAs serve important roles in the pathogenesis of OS as oncogenes or tumor suppressors. Recent studies revealed that miR-708-5p (miR-708) was dysregulated in various types of human cancer; however, its roles and underlying molecular mechanisms in OS remain unknown. Therefore, the present study aimed to determine miR-708 expression in OS, investigate the roles of miR-708 in the progression of OS and reveal the potential mechanisms involved. It was demonstrated using reverse transcription-polymerase chain reaction that miR-708 was downregulated in OS tissues and cell lines. Cell Counting Kit-8 and Transwell assays revealed that miR-708 overexpression suppressed the proliferation and invasion of OS cells in vitro. Additionally, zinc finger E-box binding homeobox 1 (ZEB1) was validated as a direct target gene of miR-708 in OS cells. ZEB1 was upregulated in OS tissues; elevated ZEB1 expression was negatively correlated with the levels of miR-708 expression. Rescue experiments indicated that ZEB1 reintroduction significantly counteracted the inhibitory effects of miR-708 overexpression on the proliferation and invasion of OS cells. The findings may improve understanding of the roles of miR-708 in the development of OS, and suggest that miR-708 may be a potential novel therapeutic target in the treatment of patients with this disease.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑708 inhibits the proliferation and invasion of osteosarcoma cells by directly targeting ZEB1

Xiang

Lin

2019

Mol Med Report

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“…Downregulating ZEB1 with a small interfering RNA mimics the effects of transfection with an miR-130a analog [81]. Similar patterns of findings are obtained with miR150, miR-708, and miR-409-3p, which target and negatively regulate ZEB1 to function as tumor suppressors in osteosarcoma [82][83][84]. Knockdown of ZEB1 inhibits, whereas ZEB1 overexpression enhances, metastasis in MG63 and SaOS-2 osteosarcoma cells, effects shown to be due to ZEB1 acting as a target of miR-708-5p [85].…”

Section: Osteosarcomasupporting

confidence: 63%

Oncogenic functions of ZEB1 in pediatric solid cancers: interplays with microRNAs and long noncoding RNAs

et al. 2021

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The transcription factor Zinc finger E-box binding 1 (ZEB1) displays a range of regulatory activities in cell function and embryonic development, including driving epithelial-mesenchymal transition. Several aspects of ZEB1 function can be regulated by its functional interactions with noncoding RNA types, namely microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). Increasing evidence indicates that ZEB1 importantly influences cancer initiation, tumor progression, metastasis, and resistance to treatment. Cancer is the main disease-related cause of death in children and adolescents. Although the role of ZEB1 in pediatric cancer is still poorly understood, emerging findings have shown that it is expressed and regulates childhood solid tumors including osteosarcoma, retinoblastoma, neuroblastoma, and central nervous system tumors. Here, we review the evidence supporting a role for ZEB1, and its interplays with miRNAs and lncRNAs, in pediatric cancers.

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“…It has been reported to be low expressed in osteosarcoma tissues and cell lines, and acted as an anti-cancer gene [9]. Besides, it is a key modulator involved in orchestrating inflammation [10].…”

Section: Introductionmentioning

confidence: 99%

Down-Regulation of MiR-150 Alleviates Inflammatory Injury Induced by Interleukin 1 via Targeting Kruppel-Like Factor 2 in Human Chondrogenic Cells

Yang

Zhang

Gao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Interleukin-1 (IL-1) is known to be involved in cartilage degeneration following joint injury or due to osteoarthritis. In the present study, we explored the effects of miR-150 on IL-1-stimulated human chondrogenic cells ATDC5. Methods: ATDC5 cells were transfected with the mimic, inhibitor or negative controls specific for miR-150, and subsequently treated by IL-1. CCK8 assay, PI and FITC-conjugated Annexin V double-staining, Western blot, qRT-PCR and ELISA assay were performed to determine the changes of cell viability, apoptosis, and the release of pro-inflammatory cytokines. Targeting relationship between miR-150 and KLF2 was detected by dual luciferase activity assay. Results: IL-1 reduced cell viability, induced apoptosis, and enhanced the expression and release of pro-inflammatory cytokines (IL-6, IL-8 and TNF-α) in ATDC5 cells. IL-1 also increased the expression of miR-150. Suppression of miR-150 alleviated IL-1-induced cell damage in ATDC5 cells, while overexpression of miR-150 resulted in an opposite impact. KLF2 was negatively regulated by miR-150, and it was proved as a target gene of miR-150. KLF2 overexpression exhibited protective actions in IL-1-injured ATDC5 cells, even if miR-150 was suppressed in cell. Moreover, IL-1-induced activation of NF-kB and Notch pathways was alleviated by KLF2 overexpression. Conclusions: Suppression of miR-150 led to up-regulation of KLF2, which in turn protected ATDC5 cells against IL-1-induced injury.

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MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma

Cited by 7 publications

References 37 publications

MicroRNA‑708 inhibits the proliferation and invasion of osteosarcoma cells by directly targeting ZEB1

MicroRNA‑708 inhibits the proliferation and invasion of osteosarcoma cells by directly targeting ZEB1

Oncogenic functions of ZEB1 in pediatric solid cancers: interplays with microRNAs and long noncoding RNAs

Down-Regulation of MiR-150 Alleviates Inflammatory Injury Induced by Interleukin 1 via Targeting Kruppel-Like Factor 2 in Human Chondrogenic Cells

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