MicroRNA-149 targets specificity protein 1 to suppress human tongue squamous cell carcinoma cell proliferation and motility

Chen, Jingxin; Chen, Jimin; Li, Weizhong

doi:10.3892/ol.2016.5527

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…Wang et al [28] showed that SP1 was the target of microRNA-149, and that microRNA-149 could play an anti-cancer role in CRC by inhibiting the expression of SP1. In our study, the expression of hsa-mir-149-5p in TSCC and in TSCC cell lines was significantly reduced, which was consistent with the results of Chen et al [22]. Their study suggests that the decreased expression of hsa-mir-149-5p is related to the proliferation and invasion of TSCC, and that the role of hsa-mir-149-5p is similar to that of tumor suppressor gene in the deterioration of TSCC.…”

Section: Discussionsupporting

confidence: 92%

“…It is still unclear whether polymorphisms of the SP1 and STAT3 genes are related to the risk of TSCC. Studies have shown that hsa-mir-149-5p can directly inhibit the expression of SP1 protein to achieve a series of biological processes such as proliferation, invasion, and cell cycle regulation of TSCC cells [22]. In addition, studies have shown that STAT3 mediates the cell survival and cisplatin (DDP) resistance of oral squamous cell carcinoma (OSCC) by up-regulating the expression of hsa-mir-21-5p and down-regulating the downstream targets of hsa-mir-21-5p, including PTEN, TIMP3, and PDCD4 [23].…”

Section: Introductionmentioning

confidence: 99%

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Association of SP1 rs1353058818 and STAT3 rs1053004 gene polymorphisms with human tongue squamous cell carcinoma

Lai

Meng³

et al. 2019

Bioscience Reports

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Objective: To study the association between SP1 rs1353058818 and STAT3 rs1053004 gene polymorphisms and risk of human tongue squamous cell carcinoma (TSCC). Methods: Sanger sequencing was used to determine the genotypes of SP1 rs1353058818 and STAT3 rs1053004 loci in 240 TSCC patients and 240 controls. Levels of hsa-miR-149-5p and hsa-miR-21-5p and expression levels of SP1 and STAT3 proteins in tumor tissues and adjacent normal tissues of TSCC patients were ascertained. Results: Carrying the SP1 rs1353058818 locus deletion allele was a high risk factor for TSCC (OR = 2.997, 95% CI: 1.389–6.466, P = 0.003). The STAT3 rs1053004 locus A allele was a protective factor for TSCC (OR = 0.604, 95% CI: 0.460-0.793, P < 0.001). There was a negative correlation between SP1 mRNA and hsa-miR-149-5p in tumor and adjacent normal tissues (r = −0.81, −0.77). The expression of SP1 protein in tumor tissues of the SP1 rs1353058818 locus DD genotype was significantly higher than in tissues of the ID type, and in tissues of type II it was the lowest. STAT3 mRNA was positively correlated with hsa-miR-21-5p in tumor and adjacent normal tissues (r = 0.75, 0.78). The expression level of STAT3 protein in tumor tissues of patients with STAT3 rs1053004 locus GG genotype was significantly higher than in patients with type GA, and it was the lowest in patients with type AA. Conclusion: Polymorphisms in the SP1 rs1353058818 and STAT3 rs1053004 loci are associated with the risk of human TSCC.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Association of SP1 rs1353058818 and STAT3 rs1053004 gene polymorphisms with human tongue squamous cell carcinoma

Lai

Meng³

et al. 2019

Bioscience Reports

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“…In recent years, it has been reported that miR-218-1-3p, as an important member of miRNAs, can inhibit the proliferation of lung cancer cells, block their cell cycle and promote apoptosis (13). In addition, it has been demonstrated that the expression of miR-149 is commonly downregulated in various malignancies, including oral squamous cell carcinoma (14), prostate cancer (15) and colorectal cancer (16), which can inhibit the invasion and migration of related cancer cells (17)(18)(19). There are also studies that have shown that lower miR-218-1-3p expression in lung cancer cells (20) and the overexpression of miR-218 can inhibit the migration and invasion of NSCLC cells, but do not affect cell growth (21).…”

Section: Introductionmentioning

confidence: 99%

Effects of miR‑218‑1‑3p and miR‑149 on proliferation and apoptosis of non‑small cell lung cancer cells

et al. 2020

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The aim of the present study was to explore the effects of miR-218-1-3p and miR-149 on the biological function of non-small cell lung cancer (NSCLC) cells A549. Paired NSCLC and adjacent tissues were obtained from 50 NSCLC patients admitted to Shandong Provincial Chest Hospital Affiliated to Shandong University (Jinan, China) from April 2015 to May 2018. The expression levels of miR-218-1-3p and miR-149 were detected by reverse transcription-quantitative PCR (RT-qPCR). The lung adenocarcinoma A549 cells were assigned into the blank group (without transfection), negative control (NC) group (transfected with miRNA NC), and the transfected groups miR-218-1-3p mimic and miR-149 mimic groups. Proliferation and cell growth were determined by CCK-8 assay and cell invasion ability in vitro was assessed by Transwell assay. Flow cytometry was carried out for the detection of cell apoptosis. RT-qPCR results showed that the expression levels of miR-218-1-3p and miR-149 in NSCLC tissues were significantly lower than those in adjacent tissues (P<0.001). At 48 and 72 h, the cell growth of the A549 cells in the miR-218-1-3p mimic and miR-149 mimic groups was significantly lower than that in the NC and blank groups (P<0.05). The number of invasive cells in the miR-218-1-3p mimic and miR-149 mimic groups was significantly lower than that in the NC and blank groups (P<0.05). The apoptotic rate of A549 cells in the miR-218-1-3p mimic and miR-149 mimic groups was significantly higher than that in the NC and blank groups (P<0.05). In conclusion, upregulation of miR-218-1-3p and miR-149 can inhibit the proliferation, invasion and migration of A549 cells in NSCLC, thereby promoting the apoptosis of A549 cells. Thus, miR-218-1-3p and miR-149 can be used as new molecular targets for the diagnosis and treatment of NSCLC.

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Genetic Polymorphisms of miR-149 Associated with Susceptibility to Both Pulmonary and Extrapulmonary Tuberculosis

Chen

Wan

Zhang

et al. 2019

Genetic Testing and Molecular Biomarkers

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MicroRNA-149 targets specificity protein 1 to suppress human tongue squamous cell carcinoma cell proliferation and motility

Cited by 6 publications

References 47 publications

Association of SP1 rs1353058818 and STAT3 rs1053004 gene polymorphisms with human tongue squamous cell carcinoma

Association of SP1 rs1353058818 and STAT3 rs1053004 gene polymorphisms with human tongue squamous cell carcinoma

Effects of miR‑218‑1‑3p and miR‑149 on proliferation and apoptosis of non‑small cell lung cancer cells

Genetic Polymorphisms of miR-149 Associated with Susceptibility to Both Pulmonary and Extrapulmonary Tuberculosis

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