MicroRNA-148b-colony-stimulating factor-1 signaling-induced tumor-associated macrophage infiltration promotes hepatocellular carcinoma metastasis

Ke, Mengyun; Zhang, Zhenhai; Cong, Longlong; Zhao, Sihai; Li, Yan; Wang, Xiaofei; Lv, Yi; Zhu, Ying; Dong, Jian

doi:10.1016/j.biopha.2019.109523

Cited by 30 publications

(29 citation statements)

References 36 publications

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“…miRNAs are single‐stranded RNA molecules that mediate gene expression at the posttranscriptional level in various processes including TAMs functions. For example, miR‐148b regulates TAMs infiltration in hepatocellular carcinoma (Ke et al, 2019). miR‐501‐3p from TAM‐derived exosomes promotes pancreatic ductal adenocarcinoma (Yin et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

M2 macrophages contribute to cell proliferation and migration of breast cancer

Dou

Wang

et al. 2021

Cell Biology International

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Breast cancer is a kind of malignant tumor that severely threatens women's lives and health worldwide. Tumor‐associated macrophages (TAMs) have been reported to mediate tumor progression, while the mechanism still needs further identification. In this study, we found that M2 macrophages promoted increased cell proliferation and migration as well as reduced expression of interferon regulatory factor 7 (IRF7) and increased the expression of miR‐1587 in breast cancer cells. Overexpression of IRF7 or miR‐1587 knockdown reversed M2 macrophage‐induced cell proliferation and migration as well as tumor growth in vivo. Mechanistically, miR‐1587 targeted the 3ʹ‐untranslated region (3ʹ‐UTR) of IRF7 mRNA to regulate its protein expression leading to tumor progression. Collectively, this study revealed that the miR‐1587/IRF7 axis mediates M2 macrophage‐induced breast cancer progression, and this sheds light on further clinical therapy for breast cancer by targeting TAMs as well as the miR‐1587/IRF7 axis.

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Section: Discussionmentioning

confidence: 99%

M2 macrophages contribute to cell proliferation and migration of breast cancer

Dou

Wang

et al. 2021

Cell Biology International

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“…2 Nevertheless, high recurrence rate and distant metastasis in HCC patient after surgery result in an unsatisfied overall survival. 3 Hence, investigation of the molecular mechanisms involved into HCC metastasis may be essential to improve the overall survival.…”

Section: Introductionmentioning

confidence: 99%

<p>Tripterine Restrains the Aggressiveness of Hepatocellular Carcinoma Cell via Regulating miRNA-532-5p/CXCL2 Axis</p>

Jiang

Han

Liu

et al. 2020

OTT

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Introduction: Triterpene has attracted considerable interests because it exhibits anticancer effects. However, the effects of tripterine on hepatocellular carcinoma (HCC) are not well studied. In the current study, the mechanism of tripterine on HCC cells growth and metastasis was examined. Methods: The inhibitory effect on the growth and aggressiveness in HCC cells was analyzed by Cell Counting Kit-8 (CCK-8), wound healing and Transwell assay. The levels of microRNA-532-5p (miR-532-5p) in HCC cells and tissues were measured using qRT-PCR. The expression of chemokine (C-X-C Motif) ligand 2 (CXCL2) was determined by Western blotting and immunohistochemistry (IHC). Luciferase reporter gene assay was used to validate the binding between miR-532-5p and CXCL2. The impact of tripterine on the growth and metastasis of HCC cells in vivo was analyzed using transplanted tumor model and experimental lung metastasis model, respectively. Results: We found that tripterine inhibited HCC cells proliferation, migration ability and invasion. Under tripterine treatment, the level of miR-532-5p was strikingly raised, and overexpression of miR-532-5p reduced cell viability and metastatic-related traits. In addition, we identified CXCL2 as a target of miR-532-5p in HCC. Rescue experiments indicated that overexpression of CXCL2 restored the migration and invasive capacity of HCC cells inhibited by miR-532-5p or tripterine treatment. Finally, the tumor growth and metastatic ability of HCC MHCC97H cell in vivo were also significantly restrained by tripterine. The expression of CXCL2 was distinctly decreased and miR-532-5p level was increased by tripterine in vivo. Conclusion:In conclusion, tripterine inhibits the growth, migration ability and invasiveness of HCC cells through intervening miR-532-5p/CXCL2.

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“…regulates TAMs in ltration in hepatocellular carcinoma (21) . TAM-derived exosomes carried miR-501-3p promotes pancreatic ductal adenocarcinoma (22) .…”

Section: Discussionmentioning

confidence: 99%

M2 macrophages contribute to cell proliferation and migration of breast cancer

Dou

Wang

et al. 2020

Preprint

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Background: Breast cancer is a kind of malignant tumor that severely threatens women’s health and life worldwide. Macrophages have been reported to mediate tumor progression, while the potential mechanism still needs further identification.Methods: Human monocytic cell line THP-1 was used to induce M2-macrophage. Real-time PCR and western blot were performed to determine gene expression in mRNA and protein level, respectively. Cell proliferation was determined using MTT assays, while cell migration was detected based on the scratch wound healing assays.Results: The supernatant medium of M2-macrophages incubated breast cancer cells showed increased cell proliferation and reduced expression of IRF-7. Overexpression of IRF-7 reversed the increased level of M2-macrophage induced cell proliferation and migration. The supernatant medium of M2-macrophages incubation promoted miR-1587 expression in breast cancer cells. miR-1587 overexpression promoted cell proliferation and migration of breast cancer. In addition, miR-1587 knockdown suppressed cell proliferation and migration that induced by M2-macrophages. miR-1587 targets IRF-7 to regulate its expression. Knockdown of IRF-7 reversed the effects of miR-1587 knockdown on cell proliferation and migration.Conclusion: Collectively, this study revealed that miR-1587/IRF-7 mediated the mechanism of M2-macrophages-induced breast cancer progression, and this would shed light on the further clinical therapy of breast cancer.

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MicroRNA-148b-colony-stimulating factor-1 signaling-induced tumor-associated macrophage infiltration promotes hepatocellular carcinoma metastasis

Cited by 30 publications

References 36 publications

M2 macrophages contribute to cell proliferation and migration of breast cancer

M2 macrophages contribute to cell proliferation and migration of breast cancer

<p>Tripterine Restrains the Aggressiveness of Hepatocellular Carcinoma Cell via Regulating miRNA-532-5p/CXCL2 Axis</p>

M2 macrophages contribute to cell proliferation and migration of breast cancer

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