MicroRNA‑145‑5p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells

He, Shuqing; Yu, Guiyuan; Peng, Ke; Liu, Sisun

doi:10.3892/mmr.2020.11592

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…Previous research has highlighted the dysregulation of miR-145-5p in various tumors, with evidence that it inhibits cancer development. [30][31][32] In our study, miR-145-5p expression was downregulated in NSCLC tissues, supporting earlier findings. 33 MiR-145-5p overexpression repressed cancer cell proliferation and metastasis, aligning with previous reports.…”

Section: Discussionsupporting

confidence: 91%

“…In line with this hypothesis, our results showed that circCPA4 bound to miR‐145‐5p to regulate NSCLC progression. Previous research has highlighted the dysregulation of miR‐145‐5p in various tumors, with evidence that it inhibits cancer development 30–32 . In our study, miR‐145‐5p expression was downregulated in NSCLC tissues, supporting earlier findings 33 .…”

Section: Discussionsupporting

confidence: 90%

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CircCPA4 induces ASCT2 expression to promote tumor property of non‐small cell lung cancer cells in a miR‐145‐5p‐dependent manner

Zhang,

Liu,

Huang

et al. 2024

Thoracic Cancer

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BackgroundNon‐small cell lung cancer (NSCLC) is a type of lung cancer that occurs in the cells of the respiratory tract, and its development is influenced by the regulation of circular RNAs (circRNAs). However, the role of circRNA carboxypeptidase A4 (circCPA4) in the progression of NSCLC and the underlying mechanism remain relatively clear.MethodsThe study utilized both real‐time quantitative polymerase chain reaction (RT‐qPCR) and western blot techniques to evaluate the levels of circCPA4, microRNA‐145‐5p (miR‐145‐5p), alanine, serine, or cysteine‐preferring transporter 2 (ASCT2). To assess cell proliferation, cell counting kit‐8 (CCK8) and 5‐ethynyl‐2′‐deoxyuridine (EdU) assays were performed. Apoptosis was determined using flow cytometry, while cell migration and invasive capacity were evaluated through transwell and wound‐healing assays. Intracellular levels of glutamine, glutamate, and α‐KG were measured using specific kits. The relationship between miR‐145‐5p and circCPA4 or ASCT2 was confirmed using dual‐luciferase reporter assay and RNA immunoprecipitation assay.ResultsCircCPA4 and ASCT2 RNA levels were elevated, while miR‐145‐5p was downregulated in both NSCLC tissues and cells. Depletion of circCPA4 significantly inhibited NSCLC cell proliferation, migration, invasion, and intracellular levels of glutamine, glutamate, and α‐KG, and promoted apoptosis. Moreover, circCPA4 knockdown delayed tumor growth in vivo. Furthermore, circCPA4 was found to bind to miR‐145‐5p, thereby regulating the progression of NSCLC in vitro. ASCT2 was also identified as a downstream target of miR‐145‐5p, and its upregulation rescued the effects of miR‐145‐5p overexpression on NSCLC cell processes.ConclusionCircCPA4 knockdown inhibited tumor property of NSCLC cells by modulating the miR‐145‐5p/ASCT2 axis.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

CircCPA4 induces ASCT2 expression to promote tumor property of non‐small cell lung cancer cells in a miR‐145‐5p‐dependent manner

Zhang,

Liu,

Huang

et al. 2024

Thoracic Cancer

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“…The m6A methylation modification is not only reported to be involved in HPV infection but also closely related to the malignancies of cervical cancer, including migration, invasion and tumour infiltration 37–40 . Previous evidence showed that FSCN1 promotes cell proliferation, migration and invasion in ovarian cancer, non‐small cell lung cancer, colorectal cancer and cervical cancer 41–45 . This evidence supported our finding that FSCN1 was a downstream effector of the HPV‐m6A modification in CSCC, and upregulated the FSCN1 advanced cell proliferation, migration and tumorigenesis of CSCC.…”

Section: Discussionsupporting

confidence: 90%

N6‐methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16‐infected cervical squamous cell carcinoma

Tian,

Huang,

Zhang

et al. 2024

Clin Exp Pharma Physio

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Human papillomavirus (HPV) infection has been reported to be associated with N6‐methyladenosine (m6A) modification in cancers. However, the underlying mechanism by which m6A methylation participates in HPV‐related cervical squamous cell carcinoma (CSCC) remains largely unclear. In this study, we observed that m6A regulators methyltransferase like protein (METTL14) and insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) were upregulated in HPV‐positive CSCC tissues and cell lines, and their high expression predicted poor prognosis for HPV‐infected CSCC patients. Cellular functional experiments verified that HPV16 oncogenes E6/E7 upregulated the expression of METTL14 and IGF2BP3 to promote cell proliferation and epithelial mesenchymal transition of CSCC cells. Next, we found that E6/E7 stabilized fascin actin‐bundling protein 1 (FSCN1) mRNA and elevated FSCN1 expression in CSCC cells through upregulating METTL14/IGF2BP3‐mediated m6A modification, and FSCN1 expression was also validated to be positively associated with worse outcomes of HPV‐positive CSCC patients. Finally, HPV16‐positive CSCC cell lines SiHa and CaSki were transfected with knockdown vector for E6/E7 or METTL14/IGF2BP3 and overexpressing vector for FSCN1, and functional verification experiments were performed through using MTT assay, flow cytometry, wound healing assay and tumour formation assay. Results indicated that knockdown of E6/E7 or METTL14/IGF2BP3 suppressed cell proliferation, migration and tumorigenesis, and accelerated cell apoptosis of HPV‐positive CSCC cells. Their tumour‐suppressive effects were abolished through overexpressing FSCN1. Overall, HPV E6/E7 advanced CSCC development through upregulating METTL14/IGF2BP3‐mediated FSCN1 m6A modification.

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“…MicroRNAs (miRNAs) are implicated in tumorigenesis by binding to the 3' UTR of target mRNA and repressing their translation. Diverse miRNAs can participate in CC development as tumor-suppressive miRNAs or oncomiRs [6][7][8][9]. For instance, miR-145-5p targets fascin to impede cancer cell migration and invasion in CC [6].…”

Section: Introductionmentioning

confidence: 99%

“…Diverse miRNAs can participate in CC development as tumor-suppressive miRNAs or oncomiRs [6][7][8][9]. For instance, miR-145-5p targets fascin to impede cancer cell migration and invasion in CC [6]. Lysyl oxidase-like protein 2 (LOXL2) belongs to the lysine oxidase gene family and is crucial in catalyzing the formation of elastin and collagen cross-links in the extracellular matrix (ECM) [10].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis

Liu

et al. 2021

Bioengineered

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Circular RNAs (circRNAs) are a class of novel non-coding RNAs that are vital in modulating gene expression and biological processes. Nevertheless, in cervical cancer (CC), the role of circRNA is much less investigated. In this work, circ_0000228 expression in CC is measured and circ_0000228's function and related mechanism are investigated. Quantitative real-time quantitative polymerase chain reaction (qRT-PCR) was utilized to examine the expression levels of circ_0000228, ) and lysyl oxidase-like protein 2 (LOXL2). Western blotting was employed to examine LOXL2 protein expression in CC cell lines. CC cell lines with circ_0000228 knockdown were constructed, and the CCK-8 experiment and Transwell experiment were executed to investigate the effect of circ_0000228 on the malignant characteristics of CC cells. Furthermore, a dual-luciferase reporter gene experiment was applied to validate the targeting relationship between circ_0000228 and In this study, we demonstrated that circ_0000228 showed a remarkable up-modulation in CC tissues and cell lines. Circ_0000228 knockdown repressed the growth and metastatic potential of CC cells. Mechanistically, circ_0000228 facilitated CC progression through sponging miR-195-5p and upmodulating LOXL2 expression. We conclude that circ_0000228 is an oncogenic circRNA, which participates in promoting CC progression via regulating the miR-195-5p/LOXL2 axis.

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MicroRNA‑145‑5p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells

Cited by 10 publications

References 30 publications

CircCPA4 induces ASCT2 expression to promote tumor property of non‐small cell lung cancer cells in a miR‐145‐5p‐dependent manner

CircCPA4 induces ASCT2 expression to promote tumor property of non‐small cell lung cancer cells in a miR‐145‐5p‐dependent manner

N6‐methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16‐infected cervical squamous cell carcinoma

Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis

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