MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs that play important roles in tumorigenesis and tumor progression through regulation of gene expression. Earlier, miR-142-3p was shown to decreased in cervical cancer cells; here; we explore the biological functional role of miR-142-3p and underlying mechanism in cervical cancer cells. We first detected the expression of miR-142-3p in six human cervical cancer cell lines and chose HeLa and SiHa cells for functional studies. By gain and loss of function experiments, we showed that overexpression of miR142-3p resulted in downregulation of Frizzled7 receptor (FZD7) and inhibited proliferation and invasion in HeLa and SiHa cells, whereas miR142-3p inhibitor-transfected cells showed reduced FZD7 expression and increased invasion capacity. In addition, we demonstrated that FZD7 was a direct target of miR-142-3p by dual luciferase assay and Western blot analysis. Overexpression of FZD7 expression was able to reverse the inhibitory effects induced by miR-142-3p. Taken together, miR-142-3p functions tumor suppressive effects in cell proliferation and invasion in cervical cancer cells, suggesting a potential therapeutic approach for cervical cancer.

Long non-coding RNA BCYRN1 promotes the proliferation and metastasis of cervical cancer via targeting microRNA-138 in�vitro and in�vivo

“…Recently some studies have reported the role of miRNAs in modulating invasion and metastasis of cervical cancer cells. For instance, Deng et al (20) discovered that miRNA-142-3p restrained cervical cancer cells proliferation and invasion, indicating a potential therapeutic approach for cervical cancer (20). Another study revealed that miR-3156-3p is reduced in cervical cancer, suggesting as a tumor-suppressive miRNA (21).…”

Downregulation of microRNA‑142‑3p and its tumor suppressor role in gastric cancer

“…For example, downregulation of miR-142-3p was observed in a large number of follicular thyroid adenomas and carcinomas, and miR-142-3p was also reported to function as a tumor suppressor in follicular thyroid tumorigenesis (16). miR-142-3p has also been reported to be able to inhibit the proliferation and invasion of cervical cancer cells by targeting frizzled-7 (30). Furthermore, lower miR-142-3p expression in hepatic cancer was associated with poorer survival.…”

MicroRNA-106a promotes cell migration and invasion by targeting tissue inhibitor of matrix metalloproteinase 2 in cervical cancer

“…Accumulating evidence has demonstrated that aberrant expression of miRNAs plays pivotal roles in the development and progression of cancer by inhibiting the expression of their target genes and potentially serve as biomarkers for the prediction and prognosis in diverse cancers, including CC (26)(27)(28). In previous studies, Zhu et al demonstrated that miR-106a targets TIMP2 to regulate the invasion and metastasis of gastric cancer (15).…”