MicroRNA‐141 promotes the proliferation of non‐small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2

Mei, Zhoufang; He, Yi; Feng, Jingjing; Shi, Jindong; Du, Yong; Qian, Ling; Huang, Qihui; Zhang, Jie

doi:10.1016/j.febslet.2014.06.020

Cited by 66 publications

(51 citation statements)

References 29 publications

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“…Inversely, in the present study, microRNA-141 level was 1.97-fold higher in NSCLC (all n = 125: SCC n = 23, ADC n = 101, and LCC n = 1) than in the paired adjacent non-cancerous lung tissues. These results were concordant with the studies with small size of NSCLC patients (all n = 70: SCC n = 36 and ADC n = 34) (Liu et al 2012) and NSCLC (n = 12) (Mei et al 2014). The number of SCC may be a factor for the contradicting results.…”

Section: Discussionsupporting

confidence: 89%

“…The number of SCC may be a factor for the contradicting results. Since the studies of Liu et al (2012), Mei et al (2014) and ours were all based on Chinese NSCLC patients, it could be possible that microRNA-141 expression may be related to the race, e.g., microRNA-141 may play a different role in the NSCLC of yellow race and white race. However, this hypothesis needs to be verified and thoroughly investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Tejero et al (2014) found that overexpression of microRNA-141 reduced KLF6 protein levels and produced an increase of secretion of VEGFA in vitro and microRNA-141 was associated with higher blood micro vessel density in patient tumor samples. Mei et al (2014) reported that microRNA-141 directly targets PH domain leucine-rich-repeats protein phosphatase 1 (PHLPP1) and PHLPP2, which enhance the proliferation of NSCLC cells by promoting cell cycle progression. The in-depth mechanism of microRNA-141 needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…However, the results were contradictory (Liu et al 2012;Mei et al 2014;Tejero et al 2014). Mei et al (2014) and Liu et al (2012) found that the expression of microRNA-141 in NSCLC tissues were up-regulated, whereas Tejero et al (2014) showed that the level of microRNA-141 in NSCLC tissues was not significantly different from that in the adjacent non-cancerous lung tissues. In addition, Liu et al (2012) found that the high expression of microRNA-141 was associated with poor prognosis of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-141 Is a Biomarker for Progression of Squamous Cell Carcinoma and Adenocarcinoma of the Lung: Clinical Analysis of 125 Patients

Zhang

Rong

et al. 2015

Tohoku J. Exp. Med.

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Lung cancer is the most common malignant tumor worldwide. MicroRNA has become an ideal biomarker for cancer diagnosis, prognosis and therapy. The relationship between microRNA-141 and non-small cell lung cancer (NSCLC) is contradictory. Thus, in current study, we aimed to investigate the level of microRNA-141 in NSCLC tissues and to evaluate its potential clinical value. This study enrolled 125 NSCLC patients (75 males and 50 females) with a median age of 61 years (range, 23-90 years). NSCLC patients included 23 squamous cell carcinomas (SCCs), 101 adenocarcinomas (ADCs) and 1 large cell carcinoma. The expression level of microRNA-141 was significantly higher in NSCLC tissues than in adjacent lung tissues (P < 0.001), detected by real time RT-PCR. Receiver operating characteristic (ROC) exhibited a moderate diagnostic value of microRNA-141 for NSCLC with the area under curve of 0.707. The microRNA-141 expression increased with the larger tumor size (P = 0.002), lymph node metastasis (P = 0.018) and advanced stage (P = 0.022) in NSCLC patients. For subgroup analysis, microRNA-141 expression in SCC was correlated with tumor size (r = 0.490, P = 0.018), and in ADC, microRNA-141 level was positively associated with tumor size (r = 0.222, P = 0.026), lymph node metastasis (r = 0.242, P = 0.015) and TNM stage (r = 0.210, P = 0.035). Furthermore, univariate analysis revealed that the expression of microRNA-141 was an independent prognostic indicator of ADC. In conclusion, microRNA-141 is a potential biomarker for the molecular diagnosis and risk stratification of NSCLC.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA-141 Is a Biomarker for Progression of Squamous Cell Carcinoma and Adenocarcinoma of the Lung: Clinical Analysis of 125 Patients

Zhang

Rong

et al. 2015

Tohoku J. Exp. Med.

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“…miR-141 was also found to down-regulate SOX17 expression and activate the WNT signal pathway, which promotes esophageal tumorigenesis [33]. Recently, Mei et al [34] reported miR-141 promoted the proliferation of non-small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-141 regulates the tumour suppressor DLC1 in colorectal cancer

Wu¹,

Zhu²,

Sun³

et al. 2015

neo

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Our previous study has showed that DLC1 acts as a functional tumor suppressor in colorectal cancer (CRC) cell lines. The aims of this study were to determine whether DLC1 is a target of MicroRNA (miRNA) regulation and to evaluate the role of this mechanism in CRC. By bioinformatics approach and literature, miR-141 was chosen for further study. The miR-141 mimic, miR-141 inhibitor were synthesized and transfected to Lovo cells. Cell growth was determined by MTT and in vivo models. The flow cytometric analysis for cell cycle determination and transwell assays for evaluating the cell invasion were used. Luciferase reporter assays and Western blots showed that DLC1 was a direct target of miR-141 in CRC. The expression levels of miR-141 were obviously up-regulated in CRC tissues compared to non-cancerous tissues, while DLC1 expression levels were down-regulated in a high proportion of clinical samples (14/18). In addition, correlation analyses revealed negative correlation between miR-141 levels and DLC1 expression levels in CRC tissues. MiR-141 overexpression promoted cell growth in vitro and in vivo, promoted cell cycle progression and invasion in Lovo cells. Furthermore, re-introduction of DLC1 in miR-141-overexpressing Lovo cells decreased growth rate of cells, increase of the percentage in G0/G1 phase and decreased the number of migrating cells. In conclusion, we demonstrated that miR-141 is up-regulated in CRC and acts as a functional oncogene by targeting DLC1.

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Retracted: MiR‐141‐3p Suppresses Tumor Growth and Metastasis in Papillary Thyroid Cancer via Targeting Yin Yang 1

Fang

Huang

et al. 2018

The Anatomical Record

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has been found to be downregulated in papillary thyroid carcinoma (PTC), while little is known about the cellular functions and precise signals elicited by miR-141-3p in PTC. The results of this study indicated that the expression of miR-141-3p was aberrantly down-regulated in PTC tissues and cell lines, compared with the adjacent normal tissues and normal thyroid epithelial cells. Furthermore, the miR-141-3p expression level was negatively associated with TNM stage and lymph node metastasis in PTC. Expression of miR-141-3p effectively inhibited cell growth, promoted apoptosis, and suppressed invasion in PTC cells. Meanwhile, miR-141-3p knockdown with miR-141-3p inhibitor reversed these effects. Consistent with the in vitro study, miR-141-3p also exhibited anti-neoplastic activity in vivo. Moreover, the results revealed that miR-141-3p directly recognized the 3 0 untranslated region (3 0 UTR) of Yin Yang 1 (YY1) and negatively regulated the expression of YY1 at both protein and mRNA levels. Ectopic expression of YY1 could effectively abrogate the anti-metastatic and proapoptotic effects of miR-141-3p. In summary, the findings suggested that miR-141-3p can act as a tumor suppressor in PTC and may be a potential therapeutic target for PTC treatment. Anat Rec, 302:258-268, 2019. -3p; YY1; tumor growth; metastasis 10%-15% of patients will relapse and develop distant metastases, leading to a poor clinical outcome (Sipos and Mazzaferri, 2010). Thus, further exploring the underlying mechanisms contributing to the occurrence, progression, and metastasis of PTC may provide insight into the novel target therapies. MicroRNAs (miRNAs) belong to a group of small noncoding RNA, and play a pivotal role in various physiological and pathological processes during cancer development

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MicroRNA‐141 promotes the proliferation of non‐small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2

Cited by 66 publications

References 29 publications

MicroRNA-141 Is a Biomarker for Progression of Squamous Cell Carcinoma and Adenocarcinoma of the Lung: Clinical Analysis of 125 Patients

MicroRNA-141 Is a Biomarker for Progression of Squamous Cell Carcinoma and Adenocarcinoma of the Lung: Clinical Analysis of 125 Patients

MicroRNA-141 regulates the tumour suppressor DLC1 in colorectal cancer

Retracted: MiR‐141‐3p Suppresses Tumor Growth and Metastasis in Papillary Thyroid Cancer via Targeting Yin Yang 1

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