MicroRNA-140-5p inhibits cell proliferation and invasion by regulating VEGFA/MMP2 signaling in glioma

Hu, Yuan; Li, Yanyan; Wu, Chun Chi; Zhou, Liang; Han, Xing; Wang, Qingyue; Xie, Xing; Zhou, Youxin; Du, Ziwei

doi:10.1177/1010428317697558

Cited by 41 publications

(29 citation statements)

References 26 publications

(40 reference statements)

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“…Glioma usually exhibits a series of malignant features, including extensive, rapid and aggressive progression, resistance to surgery, radiotherapy and chemotherapy, all of which lead to the poor prognosis for most patients 3. Although extensive efforts have been made to improve glioma treatment, average survival time of the patients remains 11‐15 months 4.…”

Section: Introductionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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This study was designed to explore the relationship between miR‐1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual‐luciferase reporter gene assay was used to validate the targeted relationship between miR‐1275 and SERPINE1. qRT‐PCR was used to detect the expression of miR‐1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway‐related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK‐8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR‐1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT‐PCR and western blot showed that miR‐1275 was low‐expressed while SERPINE1 was high‐expressed in glioma. Dual‐luciferase assay showed that miR‐1275 could bind to SERPINE1. Overexpression of miR‐1275 could promote the p53 pathway‐related proteins’ expression. Highly expressed miR‐1275 could repress the migration, proliferation and invasion of glioma cells while highly expressed SERPINE1 had inverse effects. Tumour xenograft showed that up‐regulated miR‐1275 or down‐regulated SERPINE1 could repress glioma growth in vivo. Up‐regulation of miR‐1275 activated p53 signalling pathway via regulating SERPINE1 and therefore suppressed glioma cell proliferation, invasion and migration, whereas promoted cell apoptosis.

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Section: Introductionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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“…As a key angiogenic factor, vascular endothelial growth factor (VEGF, also called VEGFA) originally isolated from tumor cells, is involved in glioma cell proliferation, angiogenesis and metastasis. Furthermore, VEGFA exerts its oncogene function may partly through its ability to promote angiogenesis . Although, angiogenesis has been shown to be important in the tumorigenesis and progression of glioma, the effects of angiogenesis on the treatment of tumors are still debated .…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, VEGFA exerts its oncogene function may partly through its ability to promote angiogenesis. [11][12][13][14] Although, angiogenesis has been shown to be important in the tumorigenesis and progression of glioma, the effects of angiogenesis on the treatment of tumors are still debated. [15][16][17][18] The multiple functions of VEGFA in the tumorigenesis of glioma need a better understanding of the underlying molecular mechanisms for glioma treatment.…”

Section: Introductionmentioning

confidence: 99%

A novel lncRNA‐LINC01116 regulates tumorigenesis of glioma by targeting VEGFA

Zhu

Chen

et al. 2019

Intl Journal of Cancer

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Brain glioma is the most common malignant tumor of the central nervous system, and one of the leading causes of death in patients with intracranial tumors. The clinical outcome of glioma is usually poor due to abundant vascularity, fast growth and susceptibility of invasion to normal brain tissues. Our microarray study showed that lncRNA‐LINC01116 was significantly upregulated in glioma tissues and played an important role in cell proliferation, cycle, migration, invasion and angiogenesis. In addition, vascular endothelial growth factor (VEGFA) may be the major target genes in the downstream of lncRNA‐LINC01116. Dual luciferase assay showed that LINC01116 and VEGFA both contained a miR‐31‐5p binding site, and LINC01116 could regulate the expression of VEGFA through competitive absorption of miR‐31‐5p. RNA immunoprecipitation indicated that LINC01116 and VEGFA were present in the miR‐31‐5p‐RISC complex, and biotinylated miR‐31‐5p pull‐down assay suggested that there was a competitive relationship between LINC01116 and VEGFA to bind with miR‐31‐5p. Collectively, our study has identified a novel lncRNA‐LINC01116 and clarified the role and mechanism of LINC01116 in the tumorigenesis of glioma. LINC01116 may prove to be a potential target for the clinical diagnosis and treatment of glioma.

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“…Dysregulation of miRNAs has been reported in almost all types of malignancy, including glioma (11), gastric cancer (12), lung cancer (13), ovarian cancer (14) and bladder cancer (15). miRNAs are involved in tumourigenesis and tumour development through participation in the regulation of diverse biological processes, including cell proliferation, cycle, apoptosis, angiogenesis, migration, invasion, metastasis and epithelial-mesenchymal transition (16)(17)(18). Therefore, further investigation into the biological role of GBM-associated miRNAs may aid the identification of novel therapeutic approaches for patients with this disease.…”

Section: Introductionmentioning

confidence: 99%

microRNA‑574 inhibits cell proliferation and invasion in glioblastoma multiforme by directly targeting zinc finger E‑box‑binding homeobox 1

Mao

Wei

et al. 2018

Mol Med Report

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Accumulated evidence has demonstrated that dysregulation of microRNAs (miRNAs) contributes to tumourigenesis and tumour development of glioblastoma multiforme (GBM). Therefore, miRNAs may be promising candidates in the development of prognosis biomarkers and effective therapeutic targets for patients with GBM. A number of studies have reported that miRNA‑574 (miR‑574) is aberrantly expressed in multiple types of human cancers. However, the expression pattern, biological functions and molecular mechanism of miR‑574 in GBM are yet to be elucidated. Therefore, the present study aimed to determine the expression level and biological functions of miR‑574 in GBM and the underlying molecular mechanisms. In the present study, miR‑574 levels were measured by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and were demonstrated to be significantly downregulated in human GBM tissues and cell lines. Functional experiments indicated that restored expression of miR‑574 using mimics led to the inhibition of the cell proliferation and invasion of GBM cells, as determined by Cell Counting kit‑8 and Matrigel invasion assays, respectively. In addition, bioinformatics analysis predicted that zinc finger E‑box‑binding homeobox 1 (ZEB1) may be a target of miR‑574. Subsequent RT‑qPCR, western blot analysis and luciferase reporter assays confirmed that ZEB1 was a direct target of miR‑574 in GBM. Additionally, ZEB1 was demonstrated to be upregulated and inversely correlated with miR‑574 expression in clinical GBM tissues. Rescue experiments demonstrated that overexpression of ZEB1 attenuated the inhibitory effects of miR‑574 on the proliferation and invasion of GBM cells. Overall, the results of the present study highlighted the potential tumour inhibitory roles of miR‑574 in GBM, thereby indicating that miR‑574 may be a novel and efficient therapeutic target for the treatment of patients with GBM.

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MicroRNA-140-5p inhibits cell proliferation and invasion by regulating VEGFA/MMP2 signaling in glioma

Cited by 41 publications

References 26 publications

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

A novel lncRNA‐LINC01116 regulates tumorigenesis of glioma by targeting VEGFA

microRNA‑574 inhibits cell proliferation and invasion in glioblastoma multiforme by directly targeting zinc finger E‑box‑binding homeobox 1

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