MicroRNA-135a Protects Against Ethanol-Induced Apoptosis in Neural Crest Cells and Craniofacial Defects in Zebrafish by Modulating the Siah1/p38/p53 Pathway

Yuan, Fuqiang; Yang, Yun; Fan, Huadong; Li, Yihong; Lü, Lei; Liu, Jie; Feng, Wenke; Chen, Shaoyu

doi:10.3389/fcell.2020.583959

Cited by 12 publications

(13 citation statements)

References 70 publications

(94 reference statements)

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“…In a rat model, third trimester-equivalent ethanol exposure results in increased variance in miRNA expression and increased expression of miR-200c , a miRNA important for neurogenesis (Balaraman et al, 2017 ). In zebrafish, miR-135a is downregulated following ethanol treatment, and its overexpression reduces the associated apoptosis in neural crest cells, growth restrictions, and craniofacial defects (Yuan et al, 2020 ).…”

Section: Epigenetics: the Interface Between Genome And Environmentmentioning

confidence: 99%

Epigenetic Impacts of Early Life Stress in Fetal Alcohol Spectrum Disorders Shape the Neurodevelopmental Continuum

Alberry

Laufer

Chater‐Diehl

et al. 2021

Front. Mol. Neurosci.

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Neurodevelopment in humans is a long, elaborate, and highly coordinated process involving three trimesters of prenatal development followed by decades of postnatal development and maturation. Throughout this period, the brain is highly sensitive and responsive to the external environment, which may provide a range of inputs leading to positive or negative outcomes. Fetal alcohol spectrum disorders (FASD) result from prenatal alcohol exposure (PAE). Although the molecular mechanisms of FASD are not fully characterized, they involve alterations to the regulation of gene expression via epigenetic marks. As in the prenatal stages, the postnatal period of neurodevelopment is also sensitive to environmental inputs. Often this sensitivity is reflected in children facing adverse conditions, such as maternal separation. This exposure to early life stress (ELS) is implicated in the manifestation of various behavioral abnormalities. Most FASD research has focused exclusively on the effect of prenatal ethanol exposure in isolation. Here, we review the research into the effect of prenatal ethanol exposure and ELS, with a focus on the continuum of epigenomic and transcriptomic alterations. Interestingly, a select few experiments have assessed the cumulative effect of prenatal alcohol and postnatal maternal separation stress. Regulatory regions of different sets of genes are affected by both treatments independently, and a unique set of genes are affected by the combination of treatments. Notably, epigenetic and gene expression changes converge at the clustered protocadherin locus and oxidative stress pathway. Functional studies using epigenetic editing may elucidate individual contributions of regulatory regions for hub genes and further profiling efforts may lead to the development of non-invasive methods to identify children at risk. Taken together, the results favor the potential to improve neurodevelopmental outcomes by epigenetic management of children born with FASD using favorable postnatal conditions with or without therapeutic interventions.

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Section: Epigenetics: the Interface Between Genome And Environmentmentioning

confidence: 99%

Epigenetic Impacts of Early Life Stress in Fetal Alcohol Spectrum Disorders Shape the Neurodevelopmental Continuum

Alberry

Laufer

Chater‐Diehl

et al. 2021

Front. Mol. Neurosci.

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show abstract

“…These results are consistent with the propensity for craniofacial defects versus the rarity of ocular anomalies in FAS. A recent study showed that miRNA-135a overexpression markedly decreased ethanol-induced apoptosis in NC cells in zebrafish embryos, and the microinjection of miRNA-135a mimics ameliorated growth retardation and craniofacial defects ( Yuan et al, 2020 ). These results suggest that microRNAs play a role in NC-mediated ocular and craniofacial development.…”

Section: Ocular Neural Crest Derivatives: Lessons From Rare Ocular DImentioning

confidence: 99%

The Ocular Neural Crest: Specification, Migration, and Then What?

Williams

Bohnsack

2020

Front. Cell Dev. Biol.

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During vertebrate embryonic development, a population of dorsal neural tube-derived stem cells, termed the neural crest (NC), undergo a series of morphogenetic changes and extensive migration to become a diverse array of cell types. Around the developing eye, this multipotent ocular NC cell population, called the periocular mesenchyme (POM), comprises migratory mesenchymal cells that eventually give rise to many of the elements in the anterior of the eye, such as the cornea, sclera, trabecular meshwork, and iris. Molecular cell biology and genetic analyses of congenital eye diseases have provided important information on the regulation of NC contributions to this area of the eye. Nevertheless, a complete understanding of the NC as a contributor to ocular development remains elusive. In addition, positional information during ocular NC migration and the molecular pathways that regulate end tissue differentiation have yet to be fully elucidated. Further, the clinical challenges of ocular diseases, such as Axenfeld-Rieger syndrome (ARS), Peters anomaly (PA) and primary congenital glaucoma (PCG), strongly suggest the need for better treatments. While several aspects of NC evolution have recently been reviewed, this discussion will consolidate the most recent current knowledge on the specification, migration, and contributions of the NC to ocular development, highlighting the anterior segment and the knowledge obtained from the clinical manifestations of its associated diseases. Ultimately, this knowledge can inform translational discoveries with potential for sorely needed regenerative therapies.

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“…In the exosomes secreted by the human diploid cell line Medical Research Council-5 (MRC-5), rabies virus infection upregulates microRNA (miR)-423-5p expression by abrogating the inhibition of cytokine signaling 3 (SOCS3) on type I interferon (IFN) signaling, resulting in feedback inhibition of RABV replication [37]. Furthermore, miR-135a family expression is downregulated to activate the p38 mitogen-activated protein kinase (MAPK)/p53 pathway, thereby contributing to apoptosis [38].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the MicroRNA Expression Profile of Exosomes Released by Vero Cells Infected with Porcine Epidemic Diarrhea Virus

Yin

Shen

Yin

et al. 2022

Viruses

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Exosomes are nanoscale vesicles actively secreted by a variety of cells. They contain regulated microRNA (miRNA), allowing them to function in intercellular communication. In the present study, the role of exosomal miRNAs in porcine epidemic diarrhea virus (PEDV) infection was investigated using exosomes isolated from Vero cells infected with PEDV. The results of transmission electron microscopy observation showed that the exosomes are spherical in shape, uniform in size, and negatively stained in the membrane. Nanoparticle tracking analysis showed that the average exosome particle size is 130.5 nm. The results of miRNA sequencing showed that, compared with the control group, a total of 115 miRNAs are abnormally expressed in the exosomes of infected cells. Of these, 80 miRNAs are significantly upregulated and 35 miRNAs are significantly downregulated. Functional annotation analysis showed that the differentially expressed miRNAs are associated with PEDV infection through interaction with the cAMP, Hippo, TGF-beta, HIF-1, FoxO, MAPK, and Ras signaling pathways. Thus, our findings provide important information about the effects of PEDV infection on exosomal miRNA expression and will aid the search for potential anti-PEDV drug candidates.

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MicroRNA-135a Protects Against Ethanol-Induced Apoptosis in Neural Crest Cells and Craniofacial Defects in Zebrafish by Modulating the Siah1/p38/p53 Pathway

Cited by 12 publications

References 70 publications

Epigenetic Impacts of Early Life Stress in Fetal Alcohol Spectrum Disorders Shape the Neurodevelopmental Continuum

Epigenetic Impacts of Early Life Stress in Fetal Alcohol Spectrum Disorders Shape the Neurodevelopmental Continuum

The Ocular Neural Crest: Specification, Migration, and Then What?

Characteristics of the MicroRNA Expression Profile of Exosomes Released by Vero Cells Infected with Porcine Epidemic Diarrhea Virus

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