MicroRNA-133a inhibits cell proliferation, colony formation ability, migration and invasion by targeting matrix metallopeptidase 9 in hepatocellular carcinoma

Abstract

Abstract. is downregulated in various types of human malignancy, including hepatocellular carcinoma (HCC), renal cell carcinoma, esophageal squamous cell carcinoma, bladder cancer, ileal carcinoid and rhabdomyosarcoma. The aim of the present study was to examine the effects of miR-133a on HCC cell proliferation, colony formation, migration and invasion. miR-133a was transfected into the HCC HepG2 and SMMC-7721 cell lines and the expression levels of miR-133a were determined; in addition, cell viability assays, colony formation assays, cell migration assays, cell invasion assays, western blot analyses and luciferase assays were performed in the HCC cell lines. The results demonstrated that miR-133a significantly inhibited cell proliferation, colony formation, migration and invasion in HepG2 and SMMC-7721 cells. To the best of our knowledge, the present study also provided the first evidence that miR-133a directly downregulated the expression of matrix metallopeptidase 9 (MMP-9) in the HCC cells. In conclusion, the results of the present study indicated that miR-133a may have suppressed cell proliferation, colony formation, migration and invasion via the downregulation of MMP-9 in HCC cell lines. Therefore, MMP-9 may be used for the development of novel molecular markers and therapeutic approaches to inhibit hepatocellular carcinoma metastasis.

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma

“…And after knockdown of hsa_circ_0001649 with siRNA in immortalized hepatoma cell lines, HCC-LM3 and MHCC-97L (Fig. 4A), we tested the expression levels of several matrix metallopeptidases (MMPs) 9, 10, 2, 13, and 7, which had been reported to have key roles in promoting the metastasis of HCC [26][27][28][29]. We found significant increase in mRNA levels of MMP9, MMP10, and MMP13 (Fig.…”

MicroRNA‑138 targets SP1 to inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells

“…miRNAs are believed to modulate more than a third of human genes (11) and therefore serve notable functions in a large number of biological processes, including cell proliferation, cell cycle, apoptosis, differentiation, metabolism, migration and invasion (12)(13)(14). Furthermore, increasing evidence has demonstrated that the abnormal expression of miRNAs contributes to the initiation and development of human malignancies, including HCC (15)(16)(17). Although numerous miRNAs have been identified, their expression and roles in HCC carcinogenesis and progression, and the underlying mechanisms by which this occurs, remain largely unidentified.…”

MicroRNA-204 suppressed proliferation and motility capacity of human hepatocellular carcinoma via directly targeting zinc finger E-box binding homeobox 2

“…Therefore, understanding the molecular mechanisms underlying the carcinogenesis and progression of HCC is required for the development of novel therapeutic treatments for patients with HCC, in order to improve the rate of survival. A number of previous studies have demonstrated that abnormal expression levels of miRNAs may be important in HCC initiation and development, suggesting that miRNA may be investigated as a novel direction in the targeted therapy of HCC (26)(27)(28).…”