MicroRNA‑127‑5p attenuates severe pneumonia via tumor necrosis factor receptor‑associated factor 1

Abstract

Pneumonia is a persistent and pervasive disease, the effects of which can be severe. MicroRNA (miR)-127-5p has been utilized as a novel biomarker for the diagnosis of severe pneumonia. The present study aimed to investigate the function of miR-127-5p during severe pneumonia. An in vitro model of severe pneumonia in Ana-1 murine macrophages was established using lipopolysaccharide (LPS). Subsequently, reverse transcription-quantitative PCR and ELISA were performed to detect the mRNA and protein expression levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α. Western blotting was also performed to measure the activity of AKT and NF-κB. The results indicated that compared with the control group, LPS treatment increased TNF receptor-associated factor 1 (TRAF1) expression levels and reduced miR-127-5p expression levels. Furthermore, the results revealed that the 3'-untranslated region of TRAF1 was targeted by miR-127-5p. miR-127-5p mimic reduced LPS-induced increases in IL-1β, IL-6 and TNF-α expression by targeting TRAF1, which was potentially mediated by inactivation of the AKT and NF-κB signaling pathways. Collectively, the results demonstrated that miR-127-5p may attenuate severe pneumonia by reducing LPS-induced inflammatory cytokine production, and inactivating the AKT and NF-κB signaling pathways by targeting TRAF1.

DNMT1-Induced miR-378a-3p Silencing Promotes Angiogenesis via NF-κB Signaling Pathway by Targeting TRAF1 in Hepatocellular Carcinoma

“…Also, miR-483 has been shown to inhibit the proliferation and migration of colorectal cancer cells by targeting TRAF1 [44]. Studies have shown that miR-127-5p alleviates severe pneumonia by targeting TRAF1 to inactivate Akt and NF-κB signaling pathways [45]. In our study, TRAF1 was con rmed as the downstream regulator of miR-378a-3p.…”

CircESPL1 silencing protects against lipopolysaccharide-induced lung fibroblast dysfunction partly by targeting the miR-146b-3p/TRAF1 axis in pneumonia

“…A previous study reported that miR-146b alleviates inflammation damage in pneumonia by modulating MyD88/NF-κB axis (17), suggesting that miR-146b played a protective role in pneumonia. miR-127-5p is reported to suppress pneumonia progression by downregulating TRAF1 (18), indicating that TRAF1 is a contributor in pneumonia pathology. In this study, we explored the intermolecular interaction and functional correlation between miR-146b-3p and circESPL1 or TRAF1 in pneumonia cell model.…”

DNMT1-induced miR-378a-3p silencing promotes angiogenesis via the NF-κB signaling pathway by targeting TRAF1 in hepatocellular carcinoma

“…Additionally, miR-483 has been shown to inhibit the proliferation and migration of colorectal cancer cells by targeting TRAF1 [ 42 ]. Studies have shown that miR-127-5p alleviates severe pneumonia by targeting TRAF1 to inactivate the Akt and NF-κB signaling pathways [ 43 ]. In our study, TRAF1 was confirmed as the downstream regulator of miR-378a-3p.…”