MicroRNA‑124 suppresses the invasion and proliferation of breast cancer cells by targeting TFAP4

Abstract

MicroRNA (miRNA/miR)-124 is widely accepted as the suppressor of different tumors. The present study aimed to improve understanding of the potential role of miR-124 in breast cancer. The gene expression profile change derived from the overexpression of miR-124 was investigated using RNA sequencing and bioinformatics analysis of the breast cancer cell line SKBR3. The results demonstrated that the gene expression profile of SKBR3 cells significantly changed. In addition, the transcription factor activating enhancer-binding protein 4 (TFAP4) gene was identified among the top 10 differentially expressed genes, and was identified as a novel target gene of miR-124 using a dual-luciferase reporter assay. TFAP4 knockdown in notably impaired SKBR3 cell migration and proliferation, which was consistent with decreasing migration and proliferation ability following overexpression of miR-124. Taken together, these results suggest that overexpression of miR-124 can suppress the migration and proliferation of SKBR3 cells by tarsgeting TFAP4. Thus, TFAP4 may act as a novel therapeutic target of breast cancer.

Multi-Omics Approaches for Revealing the Epigenetic Regulation of Histone H3.1 during Spermatogonial Stem Cell Differentiation In Vitro

“…Red and blue dots represented highly- and scarcely-expressed genes at 48 h, respectively ( Figure 4 E–H). We also revealed that the genes with relatively high expression at 0 h were primarily related to cell growth and proliferation, particularly cancer cell proliferation, such as Tfap4 [ 17 ], Rdm1 [ 18 ], Ddx39b [ 19 ], Pgam1 [ 20 ], and so on. Some of the highly expressed genes at 48 h were related to growth inhibition and differentiation, such as Maf1 [ 21 ], Zfp771 [ 22 ], Smarcd1 [ 23 ], Hoxb4 [ 24 ], and so on ( Figure 4 E).…”

Astrocytes-Derived Small Extracellular Vesicles Hinder Glioma Growth

“…In addition, miR124 overexpression reduced the cell vitality, proliferation and migration of breast cancer cells targeting flotillin-1 [ 61 ] (a protein of the non-caveolar lipid raft), correlated with the development of several tumors [ 62 , 63 ], or targeting SOX9 (sex-determining region Y-box9 protein) that is essential for self-renewal of tumor stem-like cells [ 64 ] in glioblastoma. Moreover, miR124 specifically reduced breast cancer cell invasion by targeting the transcription factor activating the enhancer-binding protein 4 V [ 65 ] and cadherin 2 [ 66 ]; in addition, miR124 reduced gastric cancer cell invasion by inhibiting the gene coding for another important protein for cell movement that is the integrin beta-3 [ 67 ]. Previous data from our group had demonstrated an enrichment of miR124 in small extracellular vesicles derived from microglia and that miR124 was involved in the anti-glioma effect exerted by these vesicles in mice [ 29 ].…”

MicroRNA (miR)-124: A Promising Therapeutic Gateway for Oncology

“…It regulates several key biological processes, such as cell proliferation, neuronal differentiation, autophagy, and inflammation [8]. miR-124 also plays a significant role in suppressing the oncogenic modification of normal cells in other tissues, like breast and pancreas [9][10][11][12][13]. Several studies have reported that down-regulation of miR-124 leads to the development of advanced cancers in the brain, breast, and colon [14,15].…”