MicroRNA-122a aggravates intestinal ischemia/reperfusion injury by promoting pyroptosis via targeting EGFR-NLRP3 signaling pathway

Wang, Fei; Gu, Lidan; Wang, Yilin; Sun, Deen; Zhao, Yuanhang; Meng, Qiang; Yin, Lianhong; Xu, Lei; Lu, Xiao‐Long; Peng, Jinrong; Ye, Lin; Sun, Pengyuan

doi:10.1016/j.lfs.2022.120863

Cited by 5 publications

(3 citation statements)

References 59 publications

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“…A study found that increasing the expression of miR-122a significantly inhibits the activity of EGFR in intestinal I/R injury, reduces the expression of EGFR mRNA and protein, and increases the expression of NLRP3 mRNA and protein. The expression of Caspase 1, N-GSDMD, ASC, IL-1β, and IL-18 proteins was upregulated to promote pyroptosis [ 66 ]. Thus, miR-122a is essential for regulating intestinal I/R injury; the study also found that miR-122a promotes pyroptosis by inhibiting the EGFR-NLRP3 signaling pathway in mice [ 66 ].…”

Section: Programmed and Non-programmed Cell Deathmentioning

confidence: 99%

“…The expression of Caspase 1, N-GSDMD, ASC, IL-1β, and IL-18 proteins was upregulated to promote pyroptosis [ 66 ]. Thus, miR-122a is essential for regulating intestinal I/R injury; the study also found that miR-122a promotes pyroptosis by inhibiting the EGFR-NLRP3 signaling pathway in mice [ 66 ]. Cor is a natural ellagitannin found in various plants and has many biological and pharmacological properties [ 67 ].…”

Section: Programmed and Non-programmed Cell Deathmentioning

confidence: 99%

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Targeting cell death pathways in intestinal ischemia-reperfusion injury: a comprehensive review

Wang,

Huang,

Wei

et al. 2024

Cell Death Discov.

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Intestinal ischemia-reperfusion (I/R) is a multifaceted pathological process, and there is a lack of clear treatment for intestinal I/R injury. During intestinal I/R, oxidative stress and inflammation triggered by cells can trigger a variety of cell death mechanisms, including apoptosis, autophagy, pyroptosis, ferroptosis, and necrosis. These cell death processes can send a danger signal for the body to be damaged and prevent intestinal I/R injury. Therefore, identifying key regulatory molecules or markers of these cell death mechanisms when intestinal I/R injury occurs may provide valuable information for the treatment of intestinal I/R injury. This paper reviews the regulatory molecules and potential markers that may be involved in regulating cell death during intestinal I/R and elaborates on the cell death mechanism of intestinal I/R injury at the molecular level to provide a theoretical basis for discovering new molecules or markers regulating cell death during intestinal I/R injury and provides ideas for drug development for the treatment of intestinal I/R injury.

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Section: Programmed and Non-programmed Cell Deathmentioning

confidence: 99%

Section: Programmed and Non-programmed Cell Deathmentioning

confidence: 99%

Targeting cell death pathways in intestinal ischemia-reperfusion injury: a comprehensive review

Wang,

Huang,

Wei

et al. 2024

Cell Death Discov.

Self Cite

View full text Add to dashboard Cite

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“…14,15 Accumulating evidence has suggested that miRNAs are key regulators of pyroptosis. [16][17][18] Recently, miR-22 was reported to play a key role in the regulation of pyroptosis in different types of cells, including endothelial cells. [19][20][21][22] However, whether miR-22 mediates pyroptosis in H/R-induced pyroptosis in endothelial cells and the underlying mechanism are unclear.…”

Section: Introductionmentioning

confidence: 99%

HSP90-Dependent Upregulation of EZH2 Promotes Hypoxia/Reoxygenation-Induced Pyroptosis by Inhibiting miR-22 in Endothelial Cells

Deng¹,

Hu²

2023

JIR

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Endothelial cell pyroptosis induced by hypoxia/reoxygenation (H/R) plays a key role in the pathogenesis of myocardial infarction (MI). However, the underlying mechanism is not clearly elucidated. Methods: Human umbilical vein endothelial cells (HUVECs) exposed to H/R acted as in vitro model to investigate the mechanism of H/R-induced endothelial cell pyroptosis. CCK-8 assays were performed to investigate the viability of HUVECs. Calcein-AM/PI staining was carried out to quantify the death of HUVECs. The expression level of miR-22 was measured by RT-qPCR. The protein expression levels of zeste 2 polycomb repressive complex 2 subunit (EZH2), NLRP3, cleaved caspase-1 (c-caspase-1), GSDMD-N and heat shock protein 90 (HSP90) were measured by Western blot. Levels of IL-1β and IL-18 in culture medium were detected by ELISA. The intracellular localization of EZH2 was detected by immunofluorescence staining. Chromatin immunoprecipitation (ChIP) assay was used to detect the enrichment of EZH2 and H3K27me3 in the miR-22 promoter region. The binding between miR-22 and NLRP3 in HUVECs was confirmed by the dual luciferase assay. Reciprocal coimmunoprecipitation was conducted to detect the direct interaction between HSP90 and EZH2. Results: H/R increased EZH2 expression, and the EZH2 siRNA could inhibit H/R-induced pyroptosis in HUVECs. H/R reduced miR-22 expression, which was reversed by EZH2 siRNA. Silencing of miR-22 by its inhibitor reversed EZH2 siRNA-induced pyroptosis inhibition in H/R-exposed HUVECs. Upregulation of miR-22 by its mimic suppressed EZH2 overexpression-enhanced pyroptosis in H/R-exposed HUVECs. ChIP assay confirmed that EZH2 bound to the miR-22 promoter region and repressed miR-22 expression through H3K27me3. Furthermore, luciferase reporter assay indicated that NLRP3 was a direct target of miR-22 in HUVECs. Finally, HSP90 siRNA inhibited H/R-induced EZH2 expression, miR-22 downregulation, and pyroptosis in HUVECs. Conclusion: H/R induces pyroptosis via the HSP90/EZH2/miR-22/NLRP3 signaling axis in endothelial cells.

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Xihuang pill facilitates glioma cell pyroptosis via the POU4F1/STAT3 axis

Tang,

Zhu,

2023

Funct Integr Genomics

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MicroRNA-122a aggravates intestinal ischemia/reperfusion injury by promoting pyroptosis via targeting EGFR-NLRP3 signaling pathway

Cited by 5 publications

References 59 publications

Targeting cell death pathways in intestinal ischemia-reperfusion injury: a comprehensive review

Targeting cell death pathways in intestinal ischemia-reperfusion injury: a comprehensive review

HSP90-Dependent Upregulation of EZH2 Promotes Hypoxia/Reoxygenation-Induced Pyroptosis by Inhibiting miR-22 in Endothelial Cells

Xihuang pill facilitates glioma cell pyroptosis via the POU4F1/STAT3 axis

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