MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1

Deng, Haoyuan; Chu, Xia; Song, Zhenyuan; Deng, Xinrui; Xu, Huan; Ye, Yaxin; Li, Songtao; Zhang, Qiao; Sun, Chao; Liu, Ying

doi:10.1159/000475571

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…Our analysis of the 10 highest ranked miRNAs acknowledged that two miRNAs, hsa-miR-1185-1 and hsa-miR-1468, among the 10 highest ranked miRNAs are not directly involved in breast cancer but are implicated in other cancers. For instance, hsa-miR-1185-1 expression was abnormally low in Alzheimer’s disease 64 and atherosclerosis 65 . The expression of hsa-miR-1468 was upregulated in hepatocellular carcinoma tissue 66 .…”

Section: Resultsmentioning

confidence: 99%

Identifying a miRNA signature for predicting the stage of breast cancer

Sathipati

2018

Sci Rep

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Breast cancer is a heterogeneous disease and one of the most common cancers among women. Recently, microRNAs (miRNAs) have been used as biomarkers due to their effective role in cancer diagnosis. This study proposes a support vector machine (SVM)-based classifier SVM-BRC to categorize patients with breast cancer into early and advanced stages. SVM-BRC uses an optimal feature selection method, inheritable bi-objective combinatorial genetic algorithm, to identify a miRNA signature which is a small set of informative miRNAs while maximizing prediction accuracy. MiRNA expression profiles of a 386-patient cohort of breast cancer were retrieved from The Cancer Genome Atlas. SVM-BRC identified 34 of 503 miRNAs as a signature and achieved a 10-fold cross-validation mean accuracy, sensitivity, specificity, and Matthews correlation coefficient of 80.38%, 0.79, 0.81, and 0.60, respectively. Functional enrichment of the 10 highest ranked miRNAs was analysed in terms of Kyoto Encyclopedia of Genes and Genomes and Gene Ontology annotations. Kaplan-Meier survival analysis of the highest ranked miRNAs revealed that four miRNAs, hsa-miR-503, hsa-miR-1307, hsa-miR-212 and hsa-miR-592, were significantly associated with the prognosis of patients with breast cancer.

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Section: Resultsmentioning

confidence: 99%

Identifying a miRNA signature for predicting the stage of breast cancer

Sathipati

2018

Sci Rep

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“…Fifteen DE miRNAs were identified on the seventh day in the tracheal tissue (as shown in Supplementary Table 6 ). At this stage, miRNAs may restore the state of the organism to normal; these included miR-379, which inhibited cell proliferation invasion ( Li et al, 2017 ), anti-inflammatory-related miR-127 ( Zhang et al, 2017 ), and apoptosis-related miR-410 ( Palumbo et al, 2016 ; Deng H. et al, 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Integrated Lung and Tracheal mRNA-Seq and miRNA-Seq Analysis of Dogs with an Avian-Like H5N1 Canine Influenza Virus Infection

Luo

et al. 2018

Front. Microbiol.

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Avian-like H5N1 canine influenza virus (CIV) causes severe respiratory infections in dogs. However, the mechanism underlying H5N1 CIV infection in dogs is unknown. The present study aimed to identify differentially expressed miRNAs and mRNAs in the lungs and trachea in H5N1 CIV-infected dogs through a next-generation sequencing-based method. Eighteen 40-day-old beagles were inoculated intranasally with CIV, A/canine/01/Guangdong/2013 (H5N1) at a tissue culture infectious dose 50 (TCID50) of 106, and lung and tracheal tissues were harvested at 3 and 7 d post-inoculation. The tissues were processed for miRNA and mRNA analysis. By means of miRNA-gene expression integrative negative analysis, we found miRNA–mRNA pairs. Lung and trachea tissues showed 138 and 135 negative miRNA–mRNA pairs, respectively. One hundred and twenty negative miRNA–mRNA pairs were found between the different tissues. In particular, pathways including the influenza A pathway, chemokine signaling pathways, and the PI3K-Akt signaling pathway were significantly enriched in all groups in responses to virus infection. Furthermore, dysregulation of miRNA and mRNA expression was observed in the respiratory tract of H5N1 CIV-infected dogs and notably, TLR4 (miR-146), NF-κB (miR-34c) and CCL5 (miR-335), CCL10 (miR-8908-5p), and GNGT2 (miR-122) were found to play important roles in regulating pathways that resist virus infection. To our knowledge, the present study is the first to analyze miRNA and mRNA expression in H5N1 CIV-infected dogs; furthermore, the present findings provide insights into the molecular mechanisms underlying influenza virus infection.

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“…It has been well documented that aberrantly expressed miRNAs are closely associated with the pathogenesis and progression of atherosclerosis [ 27 , 28 ]. miR-210 [ 29 ], miR-1185 [ 30 ], miR-19b [ 31 ], and let-7 g [ 32 ] have been reported to participate in the regulation of EC apoptosis in the setting of atherosclerosis. miR-19a [ 33 ], miR-155 [ 34 ] and miR-27 [ 35 ] were demonstrated to be able to modulate inflammatory reactions in atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

miR-26a inhibits atherosclerosis progression by targeting TRPC3

Feng

Wang

2018

Cell Biosci

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BackgroundAtherosclerosis, a chronic multi-factorial vascular disease, has become a predominant cause of a variety of cardiovascular disorders. miR-26a was previously reported to be involved in atherosclerosis progression. However, the underlying mechanism of miR-26a in atherosclerosis remains to be further explained.MethodsHigh-fat diet (HFD)-fed apolipoprotein E (apoE)−/− mice and oxidized low-density lipoprotein (ox-LDL)-stimulated human aortic endothelial cells (HAECs) were established as in vivo and in vitro models of atherosclerosis. RT-qPCR and western blot analysis were performed to measure the expression of miR-26a and transient receptor potential canonical 3 (TRPC3), respectively. Binding between miR-26a and TRPC3 was predicted with bioinformatics software and verified using a dual luciferase reporter assay. The effects of miR-26a on the lipid accumulation, atherosclerotic lesion, and inflammatory response in HFD-fed apoE−/− mice were investigated by a colorimetric enzymatic assay system, hematoxylin–eosin and oil-Red-O staining, and ELISA, respectively. Additionally, the effects of miR-26a or combined with TRPC3 on cell viability, apoptosis and the nuclear factor-kappa B (NF-κB) pathway in ox-LDL-stimulated HAECs were evaluated by MTT assay, TUNEL assay, and western blot, respectively.ResultsmiR-26a was downregulated in HFD-fed apoE−/− mice and ox-LDL-stimulated HAECs. miR-26a overexpression inhibited the pathogenesis of atherosclerosis by attenuating hyperlipidemia, atherosclerotic lesion and suppressing inflammatory response in HFD-fed apoE−/− mice. Moreover, miR-26a overexpression suppressed inflammatory response and the NF-κB pathway, promoted cell viability and inhibited apoptosis in ox-LDL-stimulated HAECs. Additionally, TRPC3 was demonstrated to be a direct target of miR-26a. Enforced expression of TRPC3 reversed the effects of miR-26a on cell viability, apoptosis, and the NF-κB pathway in ox-LDL-treated HAECs.ConclusionsmiR-26a alleviated the development of atherosclerosis by regulating TRPC3, providing a potential target for atherosclerosis treatment.

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MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1

Cited by 13 publications

References 40 publications

Identifying a miRNA signature for predicting the stage of breast cancer

Identifying a miRNA signature for predicting the stage of breast cancer

Integrated Lung and Tracheal mRNA-Seq and miRNA-Seq Analysis of Dogs with an Avian-Like H5N1 Canine Influenza Virus Infection

miR-26a inhibits atherosclerosis progression by targeting TRPC3

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