MicroRNA-106b is involved in transforming growth factor β1–induced cell migration by targeting disabled homolog 2 in cervical carcinoma

Cheng, Yuan; Guo, Yanli; Zhang, Youyi; You, Ke; Li, Zijian; Geng, Li

doi:10.1186/s13046-016-0290-6

Cited by 38 publications

(29 citation statements)

References 24 publications

(25 reference statements)

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“…Our results showing lower DAB2 expression in EBV-positive GC cell lines than in EBV-negative cells support that DAB2 functions as a tumor suppressor in EBVaGC. That is consistent with previous reports showing DAB2 as a putative tumor suppressor in various cancer types [21][22][23][24][25][26][27][28][29][30][31]. Some EBV-negative GC cells which do not express miR-BART1-3p showed no or very little expression of DAB2.…”

Section: Discussionsupporting

confidence: 93%

“…In addition, miR-93 promoted cell growth and invasion in NPC [22]. High expression of miR-106b was found in cervical cancer, and miR-106b promoted TGF-β1-induced cervical cancer cell migration by targeting DAB2 [31]. Further, miR-191 promoted the cellular viability of estrogen receptor-positive breast cancer cells by directly suppressing the expression of DAB2 [25].…”

Section: Introductionmentioning

confidence: 96%

“…Further, miR-191 promoted the cellular viability of estrogen receptor-positive breast cancer cells by directly suppressing the expression of DAB2 [25]. Additionally, the induction of DAB2 expression reduced cancer growth, migration, and invasion [21][22][23][24][25][27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

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Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2

2020

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Although Epstein-Barr virus (EBV) is known to encode over 40 different miRNAs of its own, the roles of most EBV miRNAs remain unknown. Disabled homolog 2 (DAB2) is a putative tumor suppressor, but its role in gastric carcinoma (GC), especially in EBV-associated GC, needs to be clarified. Our qRT-PCR and mRNA microarray results showed that DAB2 expression was down-regulated in EBV-positive GC cells compared to EBV-negative cells. Four BART miRNAs that might target DAB2 were predicted, and we found, using a luciferase reporter assay, that miR-BART1-3p directly targeted the 3'-UTR of DAB2. The miR-BART1-3p transfection decreased DAB2 expression at both mRNA and protein levels, while transfection of an inhibitor of miR-BART1-3p, miR-BART1-3p(i), increased DAB2 expression. In addition, miR-BART1-3p as well as siDAB2 increased migration and decreased apoptosis. Meanwhile, miR-BART1-3p(i) or pcDNA3.1-DAB2 transfection decreased migration and increased apoptosis in EBV-infected GC cells. Furthermore, decreased migration by miR-BART1-3p(i) was abrogated by co-transfected siDAB2, while decreased migration by miR-BART1-3p(i) was further suppressed by a co-transfected DAB2 over-expression vector. Our data suggest that miR-BART1-3p plays an important role in the tumorigenesis of EBV-associated GC by directly targeting DAB2.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 96%

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Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2

2020

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“…Our previous study established a related miRNA-mRNA regulatory network diagram through mRNA and microRNA chips analysis of normal cervical tissues and cervical cancer tissues and found that miR-106b is a key node in the network and closely related to cervical cancer cell migration. In addition, our previous study also found that miR-106b was significantly upregulated in cervical cancer tissues and could promote EMT and migration of cancer cells by degrading its target protein DAB2 (22).…”

Section: Introductionmentioning

confidence: 87%

Substrate stiffness affects epithelial-mesenchymal transition of cervical cancer cells through miR-106b and its target protein DAB2

Piao

You

Guo

et al. 2017

International Journal of Oncology

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The effects of different substrate stiffness were investigated on epithelial-mesenchymal transition (EMT) of cervical cancer cell lines and the role of miR-106b and its target protein DAB2 therein. Cervical cancer cell lines HeLa and SiHa were cultured on artificial substrates with different stiffness prepared using different ratios of acrylamide and bis-acrylamide. Changes of microRNA profiles were detected using microRNA chip analysis, and the expression levels of EMT-related markers E-cadherin and vimentin were detected using western blotting and real-time PCR. In addition, the effects of miR-106b overexpression as well as miR-106b and DAB2 knockdown on expression of E-cadherin and vimentin were also examined using western blotting and real-time PCR. The results showed that i) cervical cancer cell lines SiHa and HeLa cultured on substrate with stiffness of 20 kPa had the strongest EMT ability, showed the highest levels of vimentin and lowest levels of E-cadherin, compared with cells cultured on substrate with stiffness of 1 kPa; ii) miR-106b knockdown reversed the effects of substrate stiffness on EMT of cervical cancer cells, while miR-106 overexpression and DAB2 knockdown induced EMT of cervical cancer cells cultured on substrate with stiffness of 20 kPa. Overall, the results indicated that substrate stiffness could regulate EMT of cervical cancer cell lines HeLa and SiHa at least partially through miR-106b and its downstream target DAB2.

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“…To quantify the intensity of the immune reaction, the average intensity was used to represent the positive IHC rate as previously described 28…”

Section: Immunohistochemistrymentioning

confidence: 99%

Effects of 4% paraformaldehyde and modified Davidson’s fluid on the morphology and immunohistochemistry of Xiang pig testes

Wang

Meng

et al. 2020

J Toxicol Pathol

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Modified Davidson's Fluid (mDF) is a good fixative for morphological and antigen preservation. However, recent studies have shown that 4% paraformaldehyde (PFA) can better preserve the actin structure in rodent testes. It remains controversial which of these fixatives is best for testicular tissue. This study investigated the effects of both mDF and 4% PFA on the morphology and antigen preservation of Xiang pig testes using hematoxylin-eosin (HE) staining and immunohistochemistry (IHC). The stronger testis penetration of mDF compared with that of 4% PFA was primarily manifested as testicular color change and decrease in tissue weight loss. Testes fixed with 4% PFA displayed a severe shrinkage of both the tubular and interstitial compartments and the seminiferous tubule area decreased by 12.02% compared with that in mDF-fixed tissues. In contrast, IHC results showed that 4% PFA fixation achieved better IHC-positive performance than mDF fixation for antigens specifically expressed in germ cells, Leydig cells and Sertoli cells. Due to this improved antigen preservation by 4% PFA fixation, the relative immunoreactions intensity significantly increased by 39.8%, 27.8%, and 76.4%, respectively, compared with that in mDF fixation. In summary, fixation of Xiang pig testes with mDF was suitable for HE staining, while fixation with 4% PFA was more suitable for IHC.

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MicroRNA-106b is involved in transforming growth factor β1–induced cell migration by targeting disabled homolog 2 in cervical carcinoma

Cited by 38 publications

References 24 publications

Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2

Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2

Substrate stiffness affects epithelial-mesenchymal transition of cervical cancer cells through miR-106b and its target protein DAB2

Effects of 4% paraformaldehyde and modified Davidson’s fluid on the morphology and immunohistochemistry of Xiang pig testes

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