MicroRNA‑103a‑3p promotes metastasis by targeting TPD52 in salivary adenoid cystic carcinoma

Abstract

Salivary adenoid cystic carcinoma (SACC) exhibits slow continuous growth, frequent local recurrences and a high incidence of blood metastasis, with advanced lung metastasis frequently occurring and being among the primary causes of mortality. MicroRNAs (miR) serve a significant role in the initiation and development of cancer and may be tumour-specific molecular targets. However, the role of miR-103a-3p in SACC remains largely unknown. In the present study, the expression levels of miR-103a-3p and tumour protein D52 (TPD52) were detected by reverse transcription-quantitative PCR. In addition, wound-healing assays, Transwell assays and mouse models of lung metastasis were used to investigate the biological functions exerted by miR-103a-3p. The present results suggested that miR-103a-3p expression was significantly upregulated in SACC samples. Gain-of-function and loss-of-function studies in SACC cells demonstrated that miR-103a-3p acted as an oncogene by promoting tumour cell migration in vitro and lung metastasis in vivo. Dual-luciferase reporter gene assays indicated that miR-103a-3p exerted its regulatory functions by binding to the 3' untranslated region of TPD52 mRNA. TPD52 overexpression rescued the effect of miR-103a-3p on promoting SACC cell migration, suggesting that miR-103a-3p acted as an oncogene to promote cancer metastasis by directly targeting TPD52. Thus, the newly identified miR-103a-3p/TPD52 axis contributes to the understanding of SACC pathogenesis, providing insights into the identification of novel biomarkers or potential therapeutic targets in SACC.

Umbilical Cord Blood-Derived Exosomes From Very Preterm Infants With Bronchopulmonary Dysplasia Impaired Endothelial Angiogenesis: Roles of Exosomal MicroRNAs

“…Overexpression of hsa-miR-103a-3p is associated with the metastasis of breast cancer (Chang et al, 2016) and many other types of cancer (Liang et al, 2015;Fu et al, 2020).…”

Screening and Bioinformatics Analysis of Competitive Endogenous RNA Regulatory Network ––Related to Circular RNA in Breast Cancer

“…The involvement of these genes in cancer progression is supported by previous studies. Fu et al reported that TPD52, targeted by mir-103a-3p, could affect the progression of salivary adenoid cystic carcinoma [ 45 ]. Further, Shi et al found that the miR-185-5p/TPD52 axis was associated with radiation sensitivity of TNBC [ 46 ].…”

Reconstruction and analysis of the aberrant lncRNA-miRNA-mRNA network based on competitive endogenous RNA in adenoid cystic carcinoma of the salivary gland

“…Chen et al reported that the lncRNA MRPL23-AS1 promoted cystic carcinoma lung metastasis ( 30 ). In addition, miR-21 ( 31 ), miRNA-140-5p ( 32 ), miR-24-3p ( 33 ), miR-93-5p ( 34 ), miR-125a-5p ( 35 ), and miR-103a-3p ( 36 ) have been reported in cancer in recent years.…”