MicroRNA-103 Promotes Proliferation and Inhibits Apoptosis in Spinal Osteosarcoma Cells by Targeting p57

Wang, Xuesong; Lin, Yu-Chuan; Peng, Lei; Sun, Ruifu; Gong, Xiaojin; Du, Jinlong; Zhang, Xiugong

doi:10.3727/096504017x15144741233346

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…However, it should be mentioned that LATS2 is not the sole target of miR103, as several other targets have been identified in other cancer cells, including A-kinase anchoring protein 12 (AKAP12), vascular endothelial growth factor (VEGF), Wnt3a and p57 ( 36 - 38 ). Thus, it is possible that miR-103 can promote the proliferation, migration, invasion and EMT of HCC cells through other targets.…”

Section: Discussionmentioning

confidence: 99%

miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2

2018

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Improving the long-term survival of patients with hepatocellular carcinoma (HCC) remains a challenge due to metastasis and recurrence. In this study, we demonstrate that the overexpression of miR-103 in HCC cells promotes epithelial-mesenchymal transition (EMT), and is associated with an enhanced metastasis and poor outcomes, as shown by western blot analysis and immunohistochemistry. Mechanistically, using reporter luciferase assay we reveal that the serine/threonine-protein kinase, large tumor suppressor kinase 2 (LATS2), a key component of the Hippo signaling pathway, is a direct target of miR-103 in HCC cells. Transwell assay, MTT assay and western blot analysis were performed to reveal that LATS2 can counteract the functional effects of miR-103 on HCC metastasis, growth and EMT. The analyses of clinical data indicated that a high expression of miR-103 correlated with a high expression of vimentin, but with a low expression of LATS2 and E-cadherin in HCC tissues. miR-103 also reduced yes-associated protein (YAP) phosphorylation. On the whole, the findings of this study suggest that miR-103 promotes HCC metastasis and EMT by directly inhibiting LATS2. Thus, targeting miR-103/LATS2 may prove to be a promising therapeutic strategy for HCC.

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Section: Discussionmentioning

confidence: 99%

miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2

2018

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“…MiRNAs bind with 3'UTR of target gene to inhibit the translation or degradation of target mRNA [16]. Aberrant expressions of miR-103a-3p are in a variety of tumors, such as high expression in osteosarcoma [17], but low expression in glioma stem cells and glioma tissue [18]. Research found that miR-103a-3p was an important molecule in the process of glioblastoma [19].…”

Section: Introductionmentioning

confidence: 99%

MOV10 binding circ-DICER1 regulates the angiogenesis of glioma via miR-103a-3p/miR-382-5p mediated ZIC4 expression change

Zhao

Liu

et al. 2019

J Exp Clin Cancer Res

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BackgroundRNA binding proteins (RBPs) have been reported to interact with RNAs to regulate gene expression. Circular RNAs (circRNAs) are a type of endogenous non-coding RNAs, which involved in the angiogenesis of tumor. The purpose of this study is to elucidate the potential roles and molecular mechanisms of MOV10 and circ-DICER1 in regulating the angiogenesis of glioma-exposed endothelial cells (GECs).MethodsThe expressions of circ-DICER1, miR-103a-3p and miR-382-5p were detected by real-time PCR. The expressions of MOV10, ZIC4, Hsp90 and PI3K/Akt were detected by real-time PCR or western blot. The binding ability of circ-SHKBP1 and miR-544a / miR-379, ZIC4 and miR-544a / miR-379 were analyzed with Dual-Luciferase Reporter System or RIP experiment. The direct effects of ZIC4 on the Hsp90β promoter were analyzed by the ChIP experiment. The cell viability, migration and tube formation in vitro were detected by CCK-8, Transwell assay and Matrigel tube formation assay. The angiogenesis in vivo was evaluated by Matrigel plug assay. Student’s t-test (two tailed) was used for comparisons between two groups. One-way analysis of variance (ANOVA) was used for multi-group comparisons followed by Bonferroni post-hoc analysis.ResultsThe expressions of RNA binding proteins MOV10, circ-DICER1, ZIC4, and Hsp90β were up-regulated in GECs, while miR103a-3p/miR-382-5p were down-regulated. MOV10 binding circ-DICER1 regulated the cell viability, migration, and tube formation of GECs. And the effects of both MOV10 and circ-DICER1 silencing were better than the effects of MOV10 or circ-DICER1 alone silencing. In addition, circ-DICER1 acts as a molecular sponge to adsorb miR-103a-3p / miR-382-5p and impair the negative regulation of miR-103a-3p / miR-382-5p on ZIC4 in GECs. Furthermore, ZIC4 up-regulates the expression of its downstream target Hsp90β, and Hsp90 promotes the cell viability, migration, and tube formation of GECs by activating PI3K/Akt signaling pathway.ConclusionsMOV10 / circ-DICER1 / miR-103a-3p (miR-382-5p) / ZIC4 pathway plays a vital role in regulating the angiogenesis of glioma. Our findings not only provides novel mechanisms for the angiogenesis of glioma, but also provide potential targets for anti-angiogenesis therapies of glioma.Electronic supplementary materialThe online version of this article (10.1186/s13046-018-0990-1) contains supplementary material, which is available to authorized users.

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“…Moreover, miR-103a-3p was also negatively correlated with LATS1 in thyroid cancer tissues. Previous studies have shown that miR-103a-3p can activate the JNK/STAT and the mTOR signaling pathways to inhibit apoptosis by targeting p57 [28], and promote cell proliferation in gastric cancer by targeting activating transcription factor 7 [12]. These studies demonstrate that miRNAs play a key role in the complex gene regulatory networks by regulating their targets.…”

Section: Discussionmentioning

confidence: 67%

Knockdown of microRNA-103a-3p inhibits the malignancy of thyroid cancer cells through Hippo signaling pathway by upregulating LATS1

Zhang¹,

Sun²,

Wu³

et al. 2021

neo

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MicroRNA (miR)-103a-3p has been shown to be involved in the development and progression of several types of cancer. However, the role of miR-103a-3p in thyroid cancer remains unclear. This study investigated the effects of miR-103a-3p on the biological characteristics of thyroid cancer cells and related mechanisms. In the present study, we found that the expression of miR-103a-3p was increased in thyroid cancer tissues compared to that in non-cancerous tissues. Additionally, the expression of miR-103a-3p in thyroid cancer cell lines (TPC-1, SW579, BHT101, K1) was markedly higher than that in the human thyroid cell line (Nthy-ori3-1). Silencing of miR-103a-3p obviously inhibited proliferation, migration, and invasion and promoted apoptosis of BHT101 cells. miR-103a-3p upregulation promoted the proliferation, migration, and invasion and inhibited apoptosis of K1 cells. Mechanistically, LATS1was identified as a functional target of miR-103a-3p and miR-103a-3p negatively regulated LATS1 expression. miR-103a-3p knockdown (or upregulation) partially reversed the effects of LATS1 knockdown (or overexpression) on proliferation, apoptosis, migration, and invasion of thyroid cancer cells. LATS1 knockdown inhibited the phosphorylation of YAP in BHT101 cells and pro moted the nuclear translocation of YAP. Whereas, miR-103a-3p downregulation reversed the inhibitory effect of LATS1 knockdown on Hippo signaling pathway. Moreover, overexpression of LATS1 induced YAP phospho rylation in K1 cells and inhibits nuclear translocation of YAP, and the upregulation of miR-103a-3p reversed this effect. Knockdown of miR-103a-3p inhibited tumor growth and progression in vivo. Taken together, knockdown of miR-103a-3p inhibits proliferation, migration, and invasion and promotes apoptosis of thyroid cancer cells through the Hippo signaling pathway by upregulating LATS1.

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MicroRNA-103 Promotes Proliferation and Inhibits Apoptosis in Spinal Osteosarcoma Cells by Targeting p57

Cited by 11 publications

References 17 publications

miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2

miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2

MOV10 binding circ-DICER1 regulates the angiogenesis of glioma via miR-103a-3p/miR-382-5p mediated ZIC4 expression change

Knockdown of microRNA-103a-3p inhibits the malignancy of thyroid cancer cells through Hippo signaling pathway by upregulating LATS1

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