MicroRNA-100 suppresses human gastric cancer cell proliferation by targeting CXCR7

Cao, Yongfeng; Ge, Jian; Song, Zhuchen; Chen, Jia; Wu, Changping

doi:10.3892/ol.2017.7305

Cited by 19 publications

(25 citation statements)

References 30 publications

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“…The function of miR-100 is controversial. miR-100 was found to act as a tumor suppressor by deregulating its target genes in hepatocellular carcinoma [29], epithelial ovarian cancer [30], nasopharyngeal carcinoma [31], gastric cancer [32], and breast cancer [33]. However, miR-100 was also found to act as an oncogene in renal cell carcinoma [34] and acute myeloid leukemia [35].…”

Section: Discussionmentioning

confidence: 99%

miR-100 inhibits cell proliferation in mantle cell lymphoma by targeting mTOR

et al. 2020

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Background miR-100 is reported to be associated with cell proliferation and apoptosis. However, the function of miR-100 in mantle cell lymphoma (MCL) is unknown. The purpose of this study is to analyze the abnormal expression of miR-100 and mTOR in MCL together with their potential biological function and pathogenesis. Method Eighteen MCL tissue samples and 3 cell lines (Jeko-1, Mino, Granta-519) were investigated in this research study, while eighteen samples of proliferative lymphadenitis from patients and peripheral lymphocyte cells from healthy volunteers served as controls. The expression and alteration of miR-100 and mTOR mRNA were detected by RT-PCR. The expression and alteration of mTOR protein were explored by Western blot. LV-miR-100-up and LV-mTOR-RNAi were constructed and transfected by lentivirus transfection. Cell proliferation, cell apoptosis and the cell cycle were detected using CCK-8 and flow cytometry. Bioinformatics prediction software was used to predict the miR-100 target gene of mTOR. A double luciferase experiment was used to verify miR-100 targeting at the mTOR-3′-UTR. The interaction between miR-100 and mTOR was further studied using recovery experiments. GraphPad Prism 7 software (version 7.2) was used for statistical analysis, and a P value < 0.05 was considered statistically significant. Results We found that the expression of miR-100 mRNA in MCL tissues and cell lines was lower, while that of the mTOR protein was higher. There was a negative correlation between miR-100 and mTOR in both MCL tissues and cell lines. Promoting miR-100 and inhibiting mTOR could inhibit cell proliferation, induce cell apoptosis and block the cell cycle in the G1 phase. A double luciferase reporter assay showed that mTOR was one of the target genes of miR-100. The recovery experiment demonstrated that PV-mTOR-up partially set off the effect of LV-miR-100-up on decreasing mTOR expression, inhibiting proliferation, inducing apoptosis and blocking the cell cycle in G1 phase in both Jeko-1 and Mino cells. Conclusions Abnormal expression of miR-100 and mTOR was found in MCL, which included downregulation of miR-100 and upregulation of mTOR. The expression of mTOR is negatively correlated with miR-100. It may play an important role in MCL pathogenesis. miR-100 up-regulation can inhibit cell proliferation, promote cell apoptosis, and inhibit cell cycle in G1 phase by targeting the mTOR gene. miR-100 may potentially be an anti-mantle cell lymphoma gene.

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Section: Discussionmentioning

confidence: 99%

miR-100 inhibits cell proliferation in mantle cell lymphoma by targeting mTOR

et al. 2020

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“…Total protein from the CM was extracted using RIPA lysis buffer (Thermo Fisher, USA) following the manufacturer's instructions, followed by concentration quantification using the BCA kit. Afterwards, the western blot analysis was performed strictly according to the previous report [33]. Briefly, samples were resolved by gel electrophoresis, transferred onto PVDF membrane, stained with corresponding first antibodies (Abcam, UK) and followed by incubation with IRDyeR680CW-labeled second antibody (Abcam, UK).…”

Section: Characterizationmentioning

confidence: 99%

Cancer cell membrane coated silica nanoparticles loaded with ICG for tumour specific photothermal therapy of osteosarcoma

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Photothermal therapy (PTT) is a rapidly developing approach for cancer therapy, which has been widely recognized to exert high efficacy as compared to chemotherapy. However, the limited tumour homing property of currently available drug delivery systems (DDSs) is the bottleneck for the efficient delivery of photothermal agents. Here in this study, we surface modified silica nanoparticles (SLN) with the cell membrane (CM) derived from 143B cells to construct a platform (CM/SLN) capable of targeting the homogenous 143B cells. In addition, indocyanine green (ICG) as a photothermal agent was encapsulated into CM/SLN to finally construct a DDS suitable for tumour-targeted PTT of osteosarcoma. Our results revealed that CM/SLN/ICG was mono-dispersed core-shell nanoparticles with advanced stability in a physiological environment. Moreover, due to the modification of CM, CM/SLN/ICG could specifically target the homogenous 143B cells both in vitro and in vivo, which demonstrated superior anticancer efficacy when compared with either SLN/ICG or free ICG. Hence, CM/SLN/ICG could be a promising DDS for tumour targeted PTT of osteosarcoma.

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“… 6 , 7 Moreover, many miRNAs have been identified to have a connection with the initiation and progression of GC. 8 - 11 For example, miR-100 expression was strongly correlated with expression of chemokine receptor 7, which in turn promotes the progression of GC. 8 Abnormal overexpression of miR-377 downregulates the tumor suppressors, including p53, PTEN, and TIMP1 expression, and serves as a novel predictive biomarker for GC tumorigenesis and prognosis, which would be useful for the therapy of GC.…”

Section: Introductionmentioning

confidence: 99%

“… 8 - 11 For example, miR-100 expression was strongly correlated with expression of chemokine receptor 7, which in turn promotes the progression of GC. 8 Abnormal overexpression of miR-377 downregulates the tumor suppressors, including p53, PTEN, and TIMP1 expression, and serves as a novel predictive biomarker for GC tumorigenesis and prognosis, which would be useful for the therapy of GC. 9 Although abnormal expression of miRNAs has been frequently described in GC, imperfection is known regarding the precise underlying mechanisms of how miRNAs modulate the behavior of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Identification of miR-758-3p as Potential Modulator of CBX5 Expression in Gastric Cancer

Guo¹,

Zhang

Pan

et al. 2018

Technol Cancer Res Treat

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Gastric cancer is one of the most frequently diagnosed cancer types in China and also the leading causes of cancer-related death. Previous study showed chromobox 5 expression was elevated in gastric cancer, but little is known regarding the precise molecular mechanisms by which chromobox 5 expression was modulated. In this study, we revealed that chromobox 5 could promote gastric cancer cell proliferation, migration, and invasion in vitro. We screened and identified microRNA-758-3p, whose expression was downregulated in gastric cancer tissues and cell lines, which was a potential upstream molecule of chromobox 5. Upregulation of microRNA-758-3p could markedly downregulate the expression of chromobox 5. Additionally, expression of microRNA-758-3p and chromobox 5 was inversely correlated in gastric cancer tissues. Moreover, microRNA-758-3p overexpression suppressed gastric cancer cell proliferation, migration, and invasion, but these effects can be partially reversed by chromobox 5 overexpression. Collectively, our results indicate that microRNA-758-3p serves as a tumor suppressor and plays a crucial role in inhibiting the proliferation, migration, and invasion of gastric cancer via targeting chromobox 5 and implicate its potential application in cancer therapy.

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MicroRNA-100 suppresses human gastric cancer cell proliferation by targeting CXCR7

Abstract: Abstract. microRNAs (miRs) are a class of small non-coding

Cited by 19 publications

References 30 publications

miR-100 inhibits cell proliferation in mantle cell lymphoma by targeting mTOR

miR-100 inhibits cell proliferation in mantle cell lymphoma by targeting mTOR

Cancer cell membrane coated silica nanoparticles loaded with ICG for tumour specific photothermal therapy of osteosarcoma

RETRACTED: Identification of miR-758-3p as Potential Modulator of CBX5 Expression in Gastric Cancer

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