Microrheology study on matrix remodelling by osteoblasts in 3D hydrogel in vitro culture

Koivisto, Janne T.; Oelschlaeger, Claude; Menne, David; Willenbacher, Norbert; Näreoja, Tuomas

doi:10.1016/j.bonr.2022.101388

Cited by 1 publication

(1 citation statement)

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“…Inhomogeneities within the hydrogel scaffolds after preparation can cause variations in the stiffness each cell is experiencing (Soto et al, 2016). The stiffness also changes over time as the cells remodel their environment as is shown with microrheological measurements (Hafner et al, 2020;Koivisto et al, 2022). It is important to acknowledge that the cells in one culture are sensing varying stiffnesses.…”

Section: Supporting Matrixmentioning

confidence: 99%

Building blocks of microphysiological system to model physiology and pathophysiology of human heart

et al. 2023

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Microphysiological systems (MPS) are drawing increasing interest from academia and from biomedical industry due to their improved capability to capture human physiology. MPS offer an advanced in vitro platform that can be used to study human organ and tissue level functions in health and in diseased states more accurately than traditional single cell cultures or even animal models. Key features in MPS include microenvironmental control and monitoring as well as high biological complexity of the target tissue. To reach these qualities, cross-disciplinary collaboration from multiple fields of science is required to build MPS. Here, we review different areas of expertise and describe essential building blocks of heart MPS including relevant cardiac cell types, supporting matrix, mechanical stimulation, functional measurements, and computational modelling. The review presents current methods in cardiac MPS and provides insights for future MPS development with improved recapitulation of human physiology.

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Section: Supporting Matrixmentioning

confidence: 99%