Microrespirometry in Small Tissues and Organs

Sláma, Karel

doi:10.1007/978-1-4613-8239-3_5

Cited by 13 publications

(2 citation statements)

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“…Oxygen consumption was recorded on a 4-channel tensiometric unit M-1000 (Mikrotechna Co., Prague, Czechoslovakia) using the basic procedures previously described [8,10]. Improvement of the capability for long-interval recording was achieved by the addition of an electronically regulated O2 generator that operates within the respiratory vessel (Czechoslovak patent application PV-6061/89).…”

Section: Measurement Of O2 Consumptionmentioning

confidence: 99%

“…What is the actual duration of a peak? By modifying a method of microrespirographic scanning originally developed for small tissue or organ samples [8], it is now possible to monitor the respiratory rates of diapausing pupae for long periods with extremely high sensitivity (picoliters 02/min) and a stable baseline. Partial pressure of 0 2 is maintained within the respiratory vessels by utilizing a built-in oxygen generator operated by an electronic feedback system.…”

Section: Introductionmentioning

confidence: 99%

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Infradian cycles of oxygen consumption in diapausing pupae of the flesh fly, Sarcophaga crassipalpis, monitored by a scanning microrespirographic method

Sláma

Denlinger

1992

Arch Insect Biochem Physiol

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Fine details of the infradian O2 consumption cycles that characterize pupal diapause in flesh flies have been monitored by a newly designed microrespirographic method coupled with an electronically regulated O2 generator. During the 4-5 days between the peaks of elevated O2 consumption, the diapausing pupae maintained a very low and fairly constant respiratory rate (13 microl O2 x g-1.h-1). During the intercalated peaks of increased respiratory metabolism, which lasted an average of 33.6 h to 24-27 degrees C, the average maximum rate of O2 consumption was 86.9 microl.g-1.h-1, a value of 6.7 times higher than the interpeak values. The respiratory peaks started abruptly in some cases while the decline was consistently gradual. During the periods between the peaks there were no discontinuous bursts of CO2 release, a feature common to diapause in many other insects. Diapause was characteristically terminated during a peak of the O2 consumption cycle. At diapause termination O2 consumption remained at the maximum values of the peak for many hours and then gradually increased to levels characteristic of nondiapause development.

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Section: Measurement Of O2 Consumptionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%