Microrespirometric determination of the effectiveness factor and biodegradation kinetics of aerobic granules degrading 4-chlorophenol as the sole carbon source

Vital‐Jácome, Miguel; Buitrón, Germán; Moreno‐Andrade, Iván; Garcia-Rea, Victor; Thalasso, F.

doi:10.1016/j.jhazmat.2016.02.077

Cited by 13 publications

(3 citation statements)

References 59 publications

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“…Moreover, Rosenkranz et al [38] assessed the phenol biodegradation efficiency and evaluated the variations in microbial community shift with increasing phenol concentrations, from 120 to 1200 mg/L, in an anaerobic sequencing batch reactor (SBR). Their experimental results showed that the reaction time required for complete removal of inlet phenol (120-1200 mg L −1 ) was 13-220 h. Vital-Jacome et al [39] operated an aerobic SBR with granular sludge to degrade 4-chlorophenol-containing synthetic wastewater. In the first three cycles, the complete removal of 4-chlorophenol at reaction times of 84, 8 and 6 h, respectively, was achieved in their study.…”

Section: Batch Experiments For Free Cellsmentioning

confidence: 99%

Phenol Degradation Kinetics by Free and Immobilized Pseudomonas putida BCRC 14365 in Batch and Continuous-Flow Bioreactors

Lin

Cheng

2020

Processes

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Phenol degradation by Pseudomonas putida BCRC 14365 was investigated at 30 °C and a pH of 5.0–9.0 in the batch tests. Experimental results for both free and immobilized cells demonstrated that a maximum phenol degradation rate occurred at an initial pH of 7. The peak value of phenol degradation rates by the free and immobilized cells were 2.84 and 2.64 mg/L-h, respectively. Considering the culture at 20 °C, there was a lag period of approximately 44 h prior to the start of the phenol degradation for both free and immobilized cells. At the temperatures ranging from 25 to 40 °C, the immobilized cells had a higher rate of phenol degradation compared to the free cells. Moreover, the removal efficiencies of phenol degradation at the final stage were 59.3–92% and 87.5–92%, for the free and immobilized cells, respectively. The optimal temperature was 30 °C for free and immobilized cells. In the batch experiments with various initial phenol concentrations of 68.3–563.4 mg/L, the lag phase was practically negligible, and a logarithmic growth phase of a particular duration was observed from the beginning of the culture. The specific growth rate (μ) in the exponential growth phase was 0.085–0.192 h−1 at various initial phenol concentrations between 68.3 and 563.4 mg/L. Comparing experimental data with the Haldane kinetics, the biokinetic parameters, namely, maximum specific growth rate (μmax), the phenol half-saturation constant (Ks) and the phenol inhibition constant (KI), were determined to equal 0.31 h−1, 26.2 mg/L and 255.0 mg/L, respectively. The growth yield and decay coefficient of P. putida cells were 0.592 ± 4.995 × 10−3 mg cell/mg phenol and 5.70 × 10−2 ± 1.122 × 10−3 day−1, respectively. A completely mixed and continuous-flow bioreactor with immobilized cells was set up to conduct the verification of the kinetic model system. The removal efficiency for phenol in the continuous-flow bioreactor was approximately 97.7% at a steady-state condition. The experimental and simulated methodology used in this work can be applied, in the design of an immobilized cell process, by various industries for phenol-containing wastewater treatment.

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Section: Batch Experiments For Free Cellsmentioning

confidence: 99%

Phenol Degradation Kinetics by Free and Immobilized Pseudomonas putida BCRC 14365 in Batch and Continuous-Flow Bioreactors

Lin

Cheng

2020

Processes

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“…By using kinetic models, the experimental results can be used for estimating the performance of similar processes in the similar operational conditions [18,19]. An understanding of the process kinetics provides a judicious basis for the design, prediction, development, and operation of processes [20][21][22]. Kinetic models have presently used in environmental engineering for designing and optimizing the wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

Kinetic modeling of organic and nitrogen removal from domestic wastewater in a down-flow hanging sponge bioreactor

Nga¹,

Hiep

Hung

2019

Environmental Engineering Research

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A down-flow hanging sponge (DHS) bioreactor was operated for the treatment of domestic wastewater. The Stover-Kincannon model was applied for kinetic evaluation of the reactor performance during the operational period. As a result, the coefficient of determination (R 2) for straight lines of effluent concentration from the experimental data and from the predictive data of BOD5; NH4 +-N; and TN were 0.9727; 0.9883; and 0.9934, respectively. The calculation of saturation value constant (Umax-g L-1 d-1) and maximum utilization rate constant (KB-g L-1 d-1) were 56.818 and 75.034 for BOD5; 2.960 and 4.713 for NH4 +-N; 2.810 and 8.37 for TN, respectively. The study suggests that Stover-Kincannon model can be used for effective evaluation of kinetic removal of BOD5; NH4 +-N; and TN from domestic wastewater treated in a DHS bioreactor.

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“…In previous studies, a microrespirometric system was used to study the biodegradation 4-chlorophenol by aerobic granules in a SBR. It was found that 4-chlorophenol was removed at 0.9 kg/m 3 of COD per day (Vital-Jácome et al, 2016) and members of Sphingobium , Comamonadaceae and Rhizobiaceae were enriched during its degradation in the aerobic granules (Gómez-Acata et al, 2018). Thus, a better understanding of the community distribution, structure and stability of the aggregates will help to further understand and optimize granule formation in SBR and will help to improve their use in wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

Microbial community dynamics during aerobic granulation in a sequencing batch reactor (SBR)

Gómez-Basurto

Vital‐Jácome

Gómez-Acata

et al. 2019

PeerJ

Self Cite

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Microorganisms in aerobic granules formed in sequencing batch reactors (SBR) remove contaminants, such as xenobiotics or dyes, from wastewater. The granules, however, are not stable over time, decreasing the removal of the pollutant. A better understanding of the granule formation and the dynamics of the microorganisms involved will help to optimize the removal of contaminants from wastewater in a SBR. Sequencing the 16S rRNA gene and internal transcribed spacer PCR amplicons revealed that during the acclimation phase the relative abundance of Acinetobacter reached 70.8%. At the start of the granulation phase the relative abundance of Agrobacterium reached 35.9% and that of Dipodascus 89.7% during the mature granule phase. Fluffy granules were detected on day 43. The granules with filamentous overgrowth were not stable and they lysed on day 46 resulting in biomass wash-out. It was found that the reactor operation strategy resulted in stable aerobic granules for 46 days. As the reactor operations remained the same from the mature granule phase to the end of the experiment, the disintegration of the granules after day 46 was due to changes in the microbial community structure and not by the reactor operation.

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Microrespirometric determination of the effectiveness factor and biodegradation kinetics of aerobic granules degrading 4-chlorophenol as the sole carbon source

Cited by 13 publications

References 59 publications

Phenol Degradation Kinetics by Free and Immobilized Pseudomonas putida BCRC 14365 in Batch and Continuous-Flow Bioreactors

Phenol Degradation Kinetics by Free and Immobilized Pseudomonas putida BCRC 14365 in Batch and Continuous-Flow Bioreactors

Kinetic modeling of organic and nitrogen removal from domestic wastewater in a down-flow hanging sponge bioreactor

Microbial community dynamics during aerobic granulation in a sequencing batch reactor (SBR)

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