MicroR408 regulates defense response upon wounding in sweet potato

Kuo, Yun‐Wei; Lin, Jer-Sheng; Li, Yuchi; Jhu, Min-Yao; King, Yu‐Chi; Jeng, Shih‐Tong

doi:10.1093/jxb/ery381

Cited by 30 publications

(24 citation statements)

References 145 publications

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“…Previously, the miR408 family is reported to be down-regulated to target LAC2 and SVR7 which leads to promoting root elongation and chloroplast accumulation during photosynthesis, CALS6 leads to plant growth and stress tolerance (Kuo et al, 2019;Song et al, 2018). The miR164 is predominantly repressed in hybrid indicating an up-regulation in NAC1 transcription which promotes auxin signalling for lateral root development (Ding et al, 2012;Fang et al, 2014;Rosas et al, 2017).…”

Section: Differential Gene Regulation and Methylation In Hybrids Playmentioning

confidence: 99%

Genome‐wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea

Sinha

Singh

Saxena

et al. 2020

Plant Biotechnology Journal

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Hybrids are extensively used in agriculture to deliver an increase in yield, yet the molecular basis of heterosis is not well understood. Global DNA methylation analysis, transcriptome analysis and small RNA profiling were aimed to understand the epigenetic effect of the changes in gene expression level in the two hybrids and their parental lines. Increased DNA methylation was observed in both the hybrids as compared to their parents. This increased DNA methylation in hybrids showed that majority of the 24-nt siRNA clusters had higher expression in hybrids than the parents. Transcriptome analysis revealed that various phytohormones (auxin and salicylic acid) responsive hybrid-MPV DEGs were significantly altered in both the hybrids in comparison to MPV. DEGs associated with plant immunity and growth were overexpressed whereas DEGs associated with basal defence level were repressed. This antagonistic patterns of gene expression might contribute to the greater growth of the hybrids. It was also noticed that some common as well as unique changes in the regulatory pathways were associated with heterotic growth in both the hybrids. Approximately 70% and 67% of down-regulated hybrid-MPV DEGs were found to be differentially methylated in ICPH 2671 and ICPH 2740 hybrid, respectively. This reflected the association of epigenetic regulation in altered gene expressions. Our findings also revealed that miRNAs might play important roles in hybrid vigour in both the hybrids by regulating their target genes, especially in controlling plant growth and development, defence and stress response pathways. The above finding provides an insight into the molecular mechanism of pigeonpea heterosis.

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Section: Differential Gene Regulation and Methylation In Hybrids Playmentioning

confidence: 99%

Genome‐wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea

Sinha

Singh

Saxena

et al. 2020

Plant Biotechnology Journal

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“…The overexpression of miR408 in rice led to a higher pollen germination rate [ 47 ]. Overexpressed miR408 also translated to an improvement in photosynthetic performance, a higher rate of vegetative growth, and increased seed yields in A. thaliana , Oryza sativa , and Nicotiana tabacum [ 23 ]; however, the overexpression of miR408 in Ipomoea batatas resulted in a semi-dwarf phenotype [ 48 ]. In the present study, miR408-suppressed S. miltiorrhiza showed no obvious phenotype changes.…”

Section: Discussionmentioning

confidence: 99%

MiR408-SmLAC3 Module Participates in Salvianolic Acid B Synthesis in Salvia miltiorrhiza

Zou¹,

Guo²,

Yang³

et al. 2021

IJMS

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MicroRNAs (miRNAs) are important regulators of gene expression involved in plant development and abiotic stress responses. Recently, miRNAs have also been reported to be engaged in the regulation of secondary plant metabolism. However, there are few functional studies of miRNAs in medicinal plants. For this study, we obtained Sm-miR408 interference lines to investigate the function of Sm-miR408 in a medicinal model plant (Salvia miltiorrhiza). It was found that inhibiting the expression of Sm-miR408 could increase the content of salvianolic acid B and rosmarinic acid in the roots. The SmLAC3 and Sm-miR408 expression patterns were analyzed by qRT-PCR. A 5’ RLM-RACE assay confirmed that Sm-miR408 targets and negatively regulates SmLAC3. Moreover, the overexpression of SmLAC3 in S. miltiorrhiza promoted the accumulation of salvianolic acids in the roots. Furthermore, the lignin content of the roots in overexpressed SmLAC3 lines was decreased. Taken together, these findings indicated that Sm-miR408 modulates the accumulation of phenolic acids in S. miltiorrhiza by targeting SmLAC3 expression levels.

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“…The cuttings of WT and MIM319 with four to six expanded leaves transplanted to the field for 4 weeks were used for analysis. The chlorophyll content assay was performed using the third fully-expanded leaves of WT and MIM319 according to the described before ( Kuo et al, 2019 ). The total chlorophyll content was figured out according to the formula in C T = 20.29A 645 −8.05A 663 .…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of TCP Transcription Factors Family in Sweet Potato Reveals Significant Roles of miR319-Targeted TCPs in Leaf Anatomical Morphology

Ren

Zhang

et al. 2021

Front. Plant Sci.

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Plant-specific TCP transcription factors play vital roles in the controlling of growth, development, and the stress response processes. Extensive researches have been carried out in numerous species, however, there hasn’t been any information available about TCP genes in sweet potato (Ipomoea batatas L.). In this study, a genome-wide analysis of TCP genes was carried out to explore the evolution and function in sweet potato. Altogether, 18 IbTCPs were identified and cloned. The expression profiles of the IbTCPs differed dramatically in different organs or different stages of leaf development. Furthermore, four CIN-clade IbTCP genes contained miR319-binding sites. Blocking IbmiR319 significantly increased the expression level of IbTCP11/17 and resulted in a decreased photosynthetic rate due to the change in leaf submicroscopic structure, indicating the significance of IbmiR319-targeted IbTCPs in leaf anatomical morphology. A systematic analyzation on the characterization of the IbTCPs together with the primary functions in leaf anatomical morphology were conducted to afford a basis for further study of the IbmiR319/IbTCP module in association with leaf anatomical morphology in sweet potato.

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MicroR408 regulates defense response upon wounding in sweet potato

Abstract: Repression of miR408 activates the expression of three miR408 target genes, IbKCS, IbPCL, and IbGAUT, leading to activation of defense response against wounding in sweet potato.

Cited by 30 publications

References 145 publications

Genome‐wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea

Genome‐wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea

MiR408-SmLAC3 Module Participates in Salvianolic Acid B Synthesis in Salvia miltiorrhiza

Genome-Wide Identification of TCP Transcription Factors Family in Sweet Potato Reveals Significant Roles of miR319-Targeted TCPs in Leaf Anatomical Morphology

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