MicroR-542-3p can mediate ILK and further inhibit cell proliferation, migration and invasion in osteosarcoma cells

Cai, Wei; Xu, Yong; Zuo, Wenshan

doi:10.18632/aging.101698

Cited by 18 publications

(16 citation statements)

References 23 publications

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“…Since it was found that miRNAs were involved in chronic lymphocytic leukemia, more and more miRNAs were reported to be deregulated and correlated with tumorigenesis. As reported, deregulated expressions of miRNAs are associated with various cancers, including osteosarcoma, colorectal cancer, and lung cancer,8–10 which influence cancer initiation, maintenance, and metastatic progression by regulating the expressions of multiple target genes. Recently, some miRNAs have been identified to be deregulated in NSCLC samples compared with normal samples 9,10.…”

Section: Introductionmentioning

confidence: 84%

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial–mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

Wang¹,

Wang²,

Du³

et al. 2019

OTT

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Background/aims: MiR-216b and forkhead box M1 (FOXM1) were demonstrated to exert their biological effects on the development and progression of tumors. This study aimed to investigate the expression and role of miR-216b and FOXM1 in tissues and cell lines of non-small cell lung cancer (NSCLC). Methods: The expressions of miR-216b and FOXM1 in NSCLC tissues and cells were detected by qRT-PCR and Western blot analysis. Cell proliferation was measured by CCK-8 assay. Cell migration and invasion were confirmed by Transwell assay. Finally, the bioinformatics and dual-luciferase reporter assay were conducted to validate the relationship of miR-216b and FOXM1. Results: Compared with normal tissues and cells, the expression of miR-216b was obviously decreased in NSCLC tissues and cells. However, the expressions of FOXM1 mRNA and protein were significantly increased, and negatively correlated with the expression of miR-216b. Multivariate Cox’s regression analysis suggested that miR-216b or FOXM1 expression was an independent prognostic factor for patients with NSCLC. MiR-216b overexpression remarkably repressed cell proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) of NSCLC cells. The bioinformatics and dual-luciferase reporter assay validated that the 3ʹ-untranslated region (3ʹ-UTR) of FOXM1 mRNA was indeed a direct target of FOXM1. In vitro, overexpression of FOXM1 partially eliminated inhibitory effects of miR-216b on cell proliferation, migration, and invasion, whereas inhibition of FOXM1 contributed to inhibitory effects mediated by miR-216b. Conclusion: MiR-216b inhibits cell proliferation, migration, invasion, and EMT by targeting the expression of FOXM1 in human NSCLC. These findings suggested a potential therapeutic role of miR-216b in patients of NSCLC.

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Section: Introductionmentioning

confidence: 84%

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial–mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

Wang¹,

Wang²,

Du³

et al. 2019

OTT

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“…Surgical resection combined with adjuvant therapy is effective for osteosarcoma. With the development of treatment (including chemotherapy and surgery), the survival rate of osteosarcoma has increased to 65-75 % [9] . However, due to tumor recurrence and metastasis, the 5 y survival rate of patients is still very low.…”

Section: Resultsmentioning

confidence: 99%

The Mechanism of Gas Anesthetics Affecting the Proliferation, Invasion and Metastasis of Osteosarcoma Cells and its Chemotherapy Sensitivity

Zhang¹,

Sun²,

Dong³

2021

ijps

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Zhang et al.: Gas Anesthetics Affecting the Osteosarcoma Cells and its Chemotherapy SensitivityTo explore the mechanism of gas anesthetics affecting osteosarcoma cell proliferation, invasion and metastasis of osteosarcoma cells and its chemotherapy sensitivity. We collected human osteosarcoma specimens from patients undergoing surgery. The expression of epithelial-to-mesenchymal transition process related proteins fibronectin, N-cadherin, E-cadherin and beta-catenin and the expression of apoptosis-related proteins B-cell lymphoma 2, caspase-9 and caspase-3 was analyzed by western blot. The cell proliferation of each group of cells was measured by cell counting kit-8. Transwell test were carried out to detect cell invasion and metastasis. The messenger RNA expression of phosphoinositide 3-kinase/protein kinase B pathway in cells was analyzed by quantitative reverse transcription polymerase chain reaction. The apoptosis was detected by Annexin V-FITC early apoptosis detection kit and cell viability was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide. Sevoflurane decreased the protein expression of N-cadherin and increased the protein expressions of E-cadherin and beta-catenin in a dosedependent manner (p<0.05). The low concentration group had decreased cell proliferation at 12 h, 24 h, 36 h and 72 h (p<0.05). The cell proliferation of the high concentration group decreased at 24 h, 36 h and 72 h (p<0.05). The sevoflurane reduced the number of cell invasion, cell metastasis and decreased the expression of B-cell lymphoma 2 protein in a dose-dependent manner (p<0.05). It increased the protein expressions of caspase-9 and caspase-3, reduced the messenger RNA expressions of phosphoinositide 3-kinase and protein kinase B and increased cell apoptosis and decreased cell viability in a dose-dependent manner (p<0.05). Gas anesthetic (sevoflurane) inhibited the proliferation, invasion and metastasis of osteosarcoma cells by inactivating the phosphoinositide 3-kinase/protein kinase B pathway and increased the chemotherapy sensitivity of osteosarcoma cells.

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“…A great deal of research validates that miR-542-3p, as a cancer-suppressive factor, is involved in regulating the progression of various tumors. For example, miR-542-3p suppresses esophageal cancer cell metastasis by suppressing OTUB1 expression (11); miR-542-3p can target ILK to repress osteosarcoma cell multiplication, migration, and invasion (12). When it comes to BC, a study reveals that miR-542-3p can target S1PR1 to repress BC cell proliferation, migration, and invasion (10).…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0000520 is Involved in Breast Cancer Progression by Targeting miR-542-3p/S1PR1 Axis

Zhao

Wang

Jiang

et al. 2021

Preprint

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Background: CircRNAs feature prominently in breast cancer (BC) progression. This study was intended to investigate the role of hsa_circ_0000520 in BC progression.Methods: After the sample collection, quantitative real-time polymerase chain reaction (qRT-PCR) was conducted for quantifying the expressions of circ_0000520, miR-542-3p, and sphingosine-1-phosphate receptor 1 (S1PR1) mRNA. 5‐Ethynyl‐2′‐Deoxyuridine (EdU) and cell counting kit-8 (CCK-8) assays were used for measuring cell proliferation. Transwell assays were employed to detect cell migration and invasion. Western blotting was utilized for analyzing S1PR1 protein expression. Dual-luciferase reporter gene assay and RNA immunoprecipitation (RIP) assay were used to delve into the targeting relationship between circ_0000520 and miR-542-3p.Results: Circ_0000520 expression was markedly elevated in BC cell lines and tissues, and knockdown of circ_0000520 could inhibit BC cell multiplication, migration, and invasion. Circ_0000520 could target miR-542-3p to negatively regulate S1PR1 expression. S1PR1 overexpression plasmid could counteract the inhibitory effects of circ_0000520 knockdown on BC cell proliferation, migration, and invasion.Conclusion: Circ_0000520, as a cancer-promoting circRNA, participates in BC progression by regulating miR-542-3p/S1PR1 axis.

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MicroR-542-3p can mediate ILK and further inhibit cell proliferation, migration and invasion in osteosarcoma cells

Cited by 18 publications

References 23 publications

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial–mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial–mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

The Mechanism of Gas Anesthetics Affecting the Proliferation, Invasion and Metastasis of Osteosarcoma Cells and its Chemotherapy Sensitivity

Hsa_circ_0000520 is Involved in Breast Cancer Progression by Targeting miR-542-3p/S1PR1 Axis

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MicroR-542-3p can mediate ILK and further inhibit cell proliferation, migration and invasion in osteosarcoma cells

Cited by 18 publications

References 23 publications

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial&ndash;mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial&ndash;mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

The Mechanism of Gas Anesthetics Affecting the Proliferation, Invasion and Metastasis of Osteosarcoma Cells and its Chemotherapy Sensitivity

Hsa_circ_0000520 is Involved in Breast Cancer Progression by Targeting miR-542-3p/S1PR1 Axis

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<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial–mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>

<p>MiR-216b suppresses cell proliferation, migration, invasion, and epithelial–mesenchymal transition by regulating FOXM1 expression in human non-small cell lung cancer</p>