Micropuncture study of excretion of water and electrolytes by the pancreas

Mangos, J. A.; McSherry, NR

doi:10.1152/ajplegacy.1971.221.2.496

Cited by 47 publications

(23 citation statements)

References 0 publications

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“…In addition, retroperfusion with the polycation poly-L-lysine inhibited bicarbonate reabsorption at all flow rates (Fig. 6), a result which has been observed previously (9).…”

Section: Relationship Of Bicarbonate Concentration To Pancreaticflow supporting

confidence: 85%

Altered Bicarbonate Reabsorption in the Pancreas of Reserpine-Treated Rabbits—a Model for Cystic Fibrosis

et al. 1983

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“…In addition, retroperfusion with the polycation poly-L-lysine inhibited bicarbonate reabsorption at all flow rates (Fig. 6), a result which has been observed previously (9).…”

Section: Relationship Of Bicarbonate Concentration To Pancreaticflow supporting

confidence: 85%

Altered Bicarbonate Reabsorption in the Pancreas of Reserpine-Treated Rabbits—a Model for Cystic Fibrosis

et al. 1983

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“…More information about the exact nature of the mechanisms of anion secretion in the pancreas is required before this effect of reserpine can be delineated. The existence of anion pumps operating in the production of pancreatic fluid has been suggested, and it was found that polylysine, a positively charged synthetic polyamino acid which inhibited transductal reabsorption of Na+ in the rat parotid, also eliminates the transductal fluxes which modify the anionic composition of pancreatic juice (9). No difference was noted in this study, on the other hand, in the excretion patterns of Na+ and K+ in the pancreatic juice of control and reserpine-treated rats.…”

Section: Pancreas Weights and Compositionmentioning

confidence: 42%

“…In the reserpinized rat model, our findings suggest an impairment of the anion-exchange mechanism which is thought to occur in the pancreatic ducts of normal rats (9,10,13). The loss of the biphasic pattern of bicarbonate and chloride excretion after crude secretin, and the reversal in the relationship between the concentration of these two anions and flow rate after purified secretin, suggest that the exchange is either inhibited or reversed as a result of some as yet unidentified action of reserpine on the pancreatic duct cells.…”

Section: Pancreas Weights and Compositionmentioning

confidence: 68%

“…It is significant, therefore, that the disturbances in pancreatic secretion induced by reserpine in the rat also involve, primarily, reductions in water and bicarbonate outputs. Pancreatic juice is thought to be a mixture of a scanty, concentrated enzyme solution secreted by the acinar cells and of a larger volume of electrolyte-rich fluid secreted by the centroacinar or duct cells (9,13). In the CF patients, the findings of Hadorn et al (5) were explained by assuming that the "electrolyte fraction" was reduced.…”

Section: Pancreas Weights and Compositionmentioning

confidence: 99%

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The Chronically Reserpinized Rat as a Possible Model for Cystic Fibrosis. VII. Alterations in the Secretory Response to Cholecystokinin and to Secretin from the Pancreas in Vivo

Perlmutter

Martinez

et al. 1978

Pediatr Res

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Summary .isoproterenol (8). In the second model, we have demonstratedThe chronically reserpinized rat has been proposed as an animal model for cystic fibrosis on the basis of morphologic and secretory alterations in the submaxillary gland and of abnormalities in pulmonary secretions. In this investigation, the volume and composition of pancreatic juice from reserpine treated rats (0.5 mg/kg/day) have been compared to those of untreated controls after stimulation with purified cholecystokinin (0.1 pg/ kg body wt) and with crude and purified preparations of secretin (6p/100 g body wt) infused iv for 30-min periods. The results demonstrate that the treated animals secreted a significantly lower volume of pancreatic juice after stimulation with these secretagogues. Fldw rates were also significantly reduced after stimulation with cholecvstokinin and crude secretin. Protein and amylase outputs in response to cholecystokinin were smaller than in control animals after unrestricted feedings, but greater after a 24-hr fast. Total bicarbonate output was also reduced after stimulation with either crude or purified secretin and the normal excretion patterns for bicarbonate and chloride were either absent or reversed in the secretin-stimulated pancreatic juice of the treated animals. Whole pancreas homogenates from the treated animals showed significant increases in Ca++ and protein content. These results indicate that chronic administration of reserpine alters the secretion of water, protein, and bicarbonate from the rat pancreas and that it affects several of the exocrine glands involved in cystic fibrosis. These findings lend support to the concept of an animal model for the human disease. SpeculationThe chronic administration of reserpine to rats has been shown to induce alterations in salivary and pulmonary secretions which resemble those seen in patients with cystic fibrosis. The concept of an animal model has been proposed on the basis of these observations. The pancreas is another exocrine gland prominently involved in cystic fibrosis and if it can be demonstrated that reserpine administration also induces alterations in pancreatic secretion, the concept of the animal model would be strengthened considerably.The generalized exocrine gland dysfunction which is characteristic of cystic fibrosis (3) has been partially reproduced in experimental animal models induced by treating rats in a chronic fashion with certain drugs that influence the level of autonomic input into these tissues. In the first of these models, changes in the excretion of monovalent ions and of basic proteins from the rat parotid gland were observed after chronic administration of that administration of reserpine for several days induces morphologic and secretory changes in the rat submaxillary gland which resemble those of cystic fibrosis (CF) (11,12). In addition, we have shown that this procedure increases the secretion of total protein and of a specific type of glycoprotein from the lungs of the treated animals, in a manner that parallels the abn...

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“…4) 40 nl/min was 1.6 mm Hg (P < 0.005). We tested the effect of using the unstained solution because of the possibility that the anionic dye would bind and inactivate the cationic poly-amino acid (10,11). An attempt to use a cationic dye, capri blue, was not successful because the dye appeared to bind to the tubule wall at the perfusion site.…”

Section: Methodsmentioning

confidence: 99%

Interference with Feedback Control of Glomerular Filtration Rate by Furosemide, Triflocin, and Cyanide

Wright¹,

Schnermann²

1974

J. Clin. Invest.

240

110

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Micropuncture study of excretion of water and electrolytes by the pancreas

Cited by 47 publications

References 0 publications

Altered Bicarbonate Reabsorption in the Pancreas of Reserpine-Treated Rabbits—a Model for Cystic Fibrosis

Altered Bicarbonate Reabsorption in the Pancreas of Reserpine-Treated Rabbits—a Model for Cystic Fibrosis

The Chronically Reserpinized Rat as a Possible Model for Cystic Fibrosis. VII. Alterations in the Secretory Response to Cholecystokinin and to Secretin from the Pancreas in Vivo

Interference with Feedback Control of Glomerular Filtration Rate by Furosemide, Triflocin, and Cyanide

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