Micropuncture studies on the influence of antidiuretic hormone on tubular fluid reabsorption in rats with hereditary hypothalamic diabetes insipidus

Schnermann, Jürgen; Valtin, Heinz; Thurau, Klaus; Nagel, Wolfram; Horster, Michael; Fischbach, H.; Wahl, Michael; Liebau, G.

doi:10.1007/bf00586878

Cited by 44 publications

(24 citation statements)

References 15 publications

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“…The reasons for the variable blood pressure changes after vasopressin are unknown, but they may represent differences' in individual responsiveness to the given dose of vasopressin. An inhibitory effect of vasopressin on proximal tubule sodium reabsorption has not been demonstrated previously (4)(5)(6). The earlier micropuncture studies were carried out because there may be stimulation of sodium transport by vasopressin as suggested by toad bladder studies (14), but such a stimulatory effect could not be shown except in the isolated collecting tubules (15).…”

Section: Resultsmentioning

confidence: 96%

“…Because of the concomitant phosphaturia, they concluded that vasopressin exerts natriuresis by stimulating the production of cyclic AMP in the proximal tubule of the kidney, thereby reducing its sodium and phosphate reabsorption. On the other hand, the available micropuncture studies in the literature (4)(5)(6) failed to demonstrate any effect of vasopressin on proximal tubule sodium transport in the animals undergoing water diuresis. Our present micropuncture study was undertaken to reexamine whether proximal tubule transport of sodium and phosphate could be altered in thyroparathyroidectomized (TPTX) dogs by administration of vasopressin in the doses frequently employed in experimental conditions.…”

Section: Introductionmentioning

confidence: 96%

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The effect of vasopressin on phosphate transport in the proximal tubule of the dog.

Wen

1974

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

The effect of vasopressin on phosphate transport in the proximal tubule of the dog.

Wen

1974

J. Clin. Invest.

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“…Calculations based on data derived from earlier micropuncture and microdissection studies in the rat suggest that rates of net transepithelial water reabsorption well in excess of 0.50 nl Vasopressin Responsiveness of Distal Tubule and Collecting Duct mm-'min-' could be anticipated if the entire distal tubule (from the macula densa to the first junction with another renal tubule) is responsive to vasopressin. From the work of Schnermann et al (20) it can be calculated that tubular fluid is delivered to the early distal tubule at a rate of approximately 9 nl/min in a water-loaded DI rat. The average length of the distal tubule from macula densa to the first junction with another renal tubule is approximately 2.4-2.5 mm (21, and Gottschalk, personal communication).…”

Section: Resultsmentioning

confidence: 99%

Response of the Distal Tubule and Cortical Collecting Duct to Vasopressin in the Rat

Woodhall¹,

Tisher²

1973

J. Clin. Invest.

146

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A B S T R A C T Renal micropuncture observations in the rat suggest that the entire "distal tubule" (defined by the micropuncturist as that portion of the renal tubule extending between the macula densa and its first junction with another (renal tubule) may be responsive to vasopressin. However, this portion of the renal tubule contains two segments that are morphologically dissimilar. The "early" distal tubule is lined by epithelium characteristic of the distal convoluted tubule, while the "late" distal tubule is lined by epithelium characteristic of the cortical collecting duct. Thus, the present study was initiated to identify the most proximal site of action of vasopressin in the distal renal tubule.

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“…However, as the authors noted, the concentration of sodium was not measured and the increased osmolality probably resulted from an increased concentration of urea as a consequence of increased medullary urea recycling during antidiuresis. Schnermann et al (38), using the same strain of rat, did measure sodium and found that neither the fractional delivery nor the concentration of sodium was changed by vasopressin administration. The application of these data to the present question is complicated, however, by the reduced responsiveness to vasopressin recently observed in thick ascending limbs obtained from this strain of rat (39).…”

Section: Discussionmentioning

confidence: 99%

Effect of vasopressin on electrical potential difference and chloride transport in mouse medullary thick ascending limb of Henle's loop.

Hall¹,

Varney²

1980

J. Clin. Invest.

164

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A B S T R A C T Medullary thick ascending limbs ofHenle's loop of the Swiss-Webster mouse were perfused in vitro with an isotonic perfusate and a Ringer's bathing medium. In five studies, addition of a supramaximal concentration of synthetic arginine vasopressin (AVP) to the bathing medium resulted in an increase in electrical potential difference (PD) from 5.0±1.5 mV, lumen positive, to 10.7±1.4 mV (P < 0.001). When AVP was removed, the PD returned to 2.6±0.9 mV (P < 0.001), then increased again to 6.9±1.7 mV (P < 0.01) when AVP was added a second time. A significant, but submaximal, increase in PD of 2.3±0.6 mV (P < 0.05) was observed in five medullary thick ascending limbs when AVP was added to the bathing medium at a concentration of 10 AU/ml. This increase was approximately one-third of the response observed at a concentration of 100 ,uU/ml in the same tubule. No further increment in PD was observed in five medullary thick ascending limbs when the AVP concentration was increased from 100 to 1,000 ,uU/ml. In seven thick ascending limbs, the effect of AVP on PD was reproduced by the addition of 8-[p-chlorophenylthio]-cyclic 3',5'-adenosine monophosphate to the bathing medium at a final concentration of 0.1 mM. AVP increased unidirectional chloride flux from lumen to bath from 29.3±3.2 to 69.8±6.2 peq/cm per s (P < 0.01) in spite of an increase in the lumen positive PD from 1.6±0.5 mV to 7.0+0.6 mV (P < 0.001). Unidirectional chloride flux from bath to lumen was not affected by AVP. In another series of experiments, net chloride flux increased from 15.6±3.0 to 41.7±5.3 peq/cm per s (P < 0.05) after addition of AVP. The effect of AVP on hydraulic water pertneability (Lp) was examined by adding raffinose to the bathing medium in both the

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Micropuncture studies on the influence of antidiuretic hormone on tubular fluid reabsorption in rats with hereditary hypothalamic diabetes insipidus

Cited by 44 publications

References 15 publications

The effect of vasopressin on phosphate transport in the proximal tubule of the dog.

The effect of vasopressin on phosphate transport in the proximal tubule of the dog.

Response of the Distal Tubule and Cortical Collecting Duct to Vasopressin in the Rat

Effect of vasopressin on electrical potential difference and chloride transport in mouse medullary thick ascending limb of Henle's loop.

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